雌二醇对视网膜缺血-再灌注损伤后基质细胞衍生因子-1表达的影响

发布时间：2018-03-30 15:07

本文选题：视网膜　切入点：缺血-再灌注损伤　出处：《眼科新进展》2017年03期

【摘要】：目的通过观察视网膜缺血-再灌注损伤后基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)的表达情况,以及雌二醇对SDF-1的表达及调控作用,研究雌二醇对大鼠视网膜缺血-再灌注损伤的保护作用机制。方法通过升高大鼠眼压的方法建立视网膜缺血-再灌注损伤模型,应用RT-PCR和Western blot方法检测视网膜缺血-再灌注损伤后各时间点(6 h、12 h、24 h)视网膜SDF-1表达情况;腹腔注射雌二醇后观察雌二醇对视网膜缺血-再灌注损伤后SDF-1表达的影响;并且应用雌激素受体拮抗剂ICI 182-780研究雌激素受体对雌二醇诱导视网膜缺血-再灌注损伤后的SDF-1的影响。结果SDF-1 mRNA和蛋白在缺血-再灌注6 h组、缺血-再灌注12 h组和缺血-再灌注24 h组表达均增加,与正常对照组比较差异均有统计学意义(均为P0.05),并且在缺血-再灌注12 h组SDF-1表达达高峰。雌二醇预处理对视网膜缺血-再灌注具有一定的保护作用;缺血-再灌注+雌二醇组SDF-1 mRNA和蛋白表达增加,与缺血-再灌注对照组及缺血-再灌注+溶剂对照组比较差异均有统计学意义(均为P0.05)。与缺血-再灌注+雌二醇组相比较,缺血-再灌注+雌二醇+ICI 182-780组SDF-1 mRNA和蛋白表达明显降低,差异均有统计学意义(均为P0.0 5)。结论雌二醇对视网膜缺血-再灌注损伤的保护作用是通过雌激素受体介导的SDF-1 mRNA和蛋白表达增强实现的。
[Abstract]:Objective to observe the expression of stromal cell derived factor -1stromal cell-derived factor-1 SDF-1 after retinal ischemia-reperfusion injury and the effect of estradiol on the expression and regulation of SDF-1. To study the protective mechanism of estradiol on retinal ischemia-reperfusion injury in rats. Methods the model of retinal ischemia-reperfusion injury was established by increasing intraocular pressure in rats. RT-PCR and Western blot were used to detect the expression of SDF-1 in retina at different time points after ischemia reperfusion injury, and the effect of estradiol on SDF-1 expression in retina after ischemia reperfusion injury was observed after intraperitoneal injection of estradiol. Estrogen receptor antagonist ICI 182-780 was used to study the effect of estrogen receptor on SDF-1 induced by estradiol in retinal ischemia reperfusion injury. Results SDF-1 mRNA and protein were treated with SDF-1 mRNA and protein for 6 h after ischemia reperfusion. The expression of protein was increased in 12 h and 24 h groups, respectively. Compared with the control group, the difference was statistically significant (P 0.05), and the expression of SDF-1 reached the peak at 12 h after ischemia-reperfusion. Estradiol preconditioning had a certain protective effect on retinal ischemia-reperfusion. The expression of SDF-1 mRNA and protein in the ischemia-reperfusion estradiol group was significantly higher than that in the ischemia-reperfusion control group and the ischemia-reperfusion solvent control group (P 0.05), and was significantly higher than that in the ischemia-reperfusion estradiol group. The expression of SDF-1 mRNA and protein in ICI 182-780 group decreased significantly after ischemia reperfusion. Conclusion the protective effect of estradiol on retinal ischemia-reperfusion injury is mediated by the enhanced expression of SDF-1 mRNA and protein mediated by estrogen receptor.
【作者单位】：哈尔滨医科大学附属第一医院眼科;
【基金】：黑龙江省卫生计生委科研课题(编号:2014-299) 黑龙江省博士后科研启动金(编号:LBHQ15101)~~
【分类号】：R774.1

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