高危型人乳头瘤病毒E7 mRNA与喉病变的关系

发布时间：2018-04-15 14:52

本文选题：人乳头瘤病毒 + 反转录实时荧光定量聚合酶链反应(PCR)　；参考：《中国老年学杂志》2017年12期

【摘要】：目的检测高危型人乳头瘤病毒(HPV)E7 mRNA表达量并分析其与喉病变的关系。方法经临床明确诊断的喉癌50例、喉癌前病变22例、声带息肉16例共计88例患者,分为喉癌组、病变组、息肉组,分别进行HPV DNA检测及HPV mRNA反转录实时荧光定量聚合酶链反应(PCR)检测。结果各组均有HPV-16及HPV-18型特异的E6、E7阳性表达。喉癌组HPV-16和HPV-18的E6及E7的相对表达量均明显高于病变组和息肉组(P0.05);喉癌组HPV-16和HPV-18的E6及E7检出率明显高于病变组和息肉组(P0.05);喉癌组HPV mRNA拷贝数明显高于病变组和息肉组(P0.05),HPV mRNA的表达水平随喉病变的发展而提高。结论 HPV mRNA的表达率随喉病变的发展而增加,其表达量也随之增强,其表达水平与喉病变发展具有一定相关性。检测HPV E7 mRNA可以为喉癌的预防、发展监测提供重要的临床指导。
[Abstract]:Objective to detect the expression of HPV-E7 mRNA in high risk human papillomavirus (HPV) and analyze its relationship with laryngeal lesions.Methods 50 cases of laryngeal carcinoma, 22 cases of laryngeal precancerous lesion and 16 cases of vocal cord polyp were divided into laryngeal carcinoma group, lesion group and polyp group.HPV DNA detection and HPV mRNA reverse transcription real-time fluorescence quantitative polymerase chain reaction (PCR) were performed.Results HPV-16 and HPV-18 specific E 6 E 7 positive expression were found in all groups.The relative expression of E6 and E7 in HPV-16 and HPV-18 in laryngeal carcinoma group was significantly higher than that in pathological group and polyp group (P0.05), the detection rate of E6 and E7 in HPV-16 and HPV-18 in laryngeal carcinoma group was significantly higher than that in pathological group and polyp group (P0.05), the copy number of HPV mRNA in laryngeal carcinoma group was significantly higher than that in pathological group and polyp group.The expression level of P0.05 mRNA in polyp group increased with the development of laryngeal lesions.Conclusion the expression rate of HPV mRNA increases with the development of laryngeal lesions, and the expression level of HPV mRNA increases with the development of laryngeal lesions.Detection of HPV E 7 mRNA may provide important clinical guidance for the prevention and development of laryngeal cancer.
【作者单位】：海南医学院附属医院耳鼻咽喉科;
【基金】：海南省医药卫生科研项目(No.2014-54)
【分类号】：R739.65

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