微小RNA-140-5p在老年下咽癌组织中的表达及对下咽癌细胞株FaDu迁移、侵袭力的影响

发布时间：2018-04-19 23:01

本文选题：下咽癌 + 微小RNAs　；参考：《中国老年学杂志》2017年07期

【摘要】：目的探讨微小RNA-140-5p(MiR-140-5p)影响去整合素金属蛋白酶(ADAM)10表达对老年下咽癌组织和细胞的作用。方法实时荧光定量RCR检测MiR-140-5p和ADAM10在41例老年下咽癌患者肿瘤组织及癌旁组织中的表达;利用转染技术分别上调MiR-140-5p表达,下调MiR-140-5p和ADAM10表达;采用Transwell细胞侵袭/迁移实验检测MiR-140-5p上调、MiR-140-5p下调、ADAM10下调对FaDu细胞迁移、侵袭的影响;Western印迹检测41对组织标本和FaDu细胞的ADAM10蛋白表达。结果 MiR-140-5p在下咽癌组织中明显下调,ADAM10 mRNA和蛋白在下咽癌组织中均明显上调。实验组FaDu细胞干扰ADAM10表达后,ADAM10表达水平明显低于阴性对照组(P0.05)。Transwell实验显示,迁移和侵袭实验中MiR-140-5p上调组每视野下平均迁移细胞数均明显低于对照组,MiR-140-5p下调组均明显高于对照组(P0.05);ADAM10干扰实验组迁移和侵袭细胞数明显低于对照组(P0.05)。ADAM10下调可逆转MiR-140-5p下调对细胞迁移和侵袭能力的促进作用。Western印迹显示,MiR-140-5p表达上调后,实验组ADAM10蛋白表达水平明显低于阴性对照组;MiR-140-5p表达下调后,实验组ADAM10蛋白表达水平明显高于阴性对照组。结论 MiR-140-5p在老年下咽癌组织中低表达,ADAM10高表达。MiR-140-5p抑制下咽癌细胞迁移、侵袭力可通过下调ADAM10蛋白表达实现,MiR-140-5p可作为下咽癌治疗的新靶点。
[Abstract]:Objective to investigate the effect of microRNA-140-5pmMiR-140-5p) on the expression of desintegrin metalloproteinase (ADAMn10) in elderly patients with hypopharyngeal carcinoma. Methods Real-time quantitative RCR was used to detect the expression of MiR-140-5p and ADAM10 in 41 elderly patients with hypopharyngeal carcinoma, and the expression of MiR-140-5p was up-regulated and the expression of MiR-140-5p and ADAM10 was down-regulated by transfection technique. Transwell cell invasion / migration assay was used to detect the up-regulation of MiR-140-5p by MiR-140-5p down-regulating the down-regulation of ADAM10 on the migration of FaDu cells. Western blot was used to detect the expression of ADAM10 protein in 41 tissues and FaDu cells. Results MiR-140-5p significantly down-regulated ADAM10 mRNA and up-regulated protein in hypopharyngeal carcinoma tissues. The level of ADAM10 expression in FaDu cells of experimental group was significantly lower than that in negative control group after interfering with ADAM10 expression. In the migration and invasion experiment, the average number of migration cells in the up-regulated group of MiR-140-5p was significantly lower than that in the down-regulated group of MiR-140-5p in the control group, which was significantly higher than that in the control group in which P0.05ADAM10 interfered with migration and invasion cells. The down-regulation of P0.05U. ADAM10 in the experimental group was significantly lower than that in the control group. Effects of MiR-140-5p down-regulation on cell migration and invasion. Western blot showed that MiR-140-5p expression was up-regulated. The expression of ADAM10 protein in the experimental group was significantly lower than that in the negative control group after down-regulation of MiR-140-5p, and the expression level of ADAM10 protein in the experimental group was significantly higher than that in the negative control group. Conclusion the low expression of ADAM10 and the high expression of MiR-140-5p in elderly hypopharyngeal carcinoma can inhibit the migration of hypopharyngeal carcinoma cells. The invasiveness can be realized by down-regulating the expression of ADAM10 protein. MiR-140-5p can be used as a new target for the treatment of hypopharyngeal carcinoma.
【作者单位】：甘肃省人民医院耳鼻咽喉头颈外科;
【分类号】：R739.63

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