豚鼠耳蜗血管纹TMEM16A随鼠增龄的表达变化

发布时间：2018-04-20 19:18

本文选题：跨膜蛋白A(TMEMA) + 血管纹　；参考：《西安交通大学学报(医学版)》2017年06期

【摘要】：目的探讨跨膜蛋白16A(transmembrane protein 16A,TMEM16A)在不同鼠龄豚鼠耳蜗血管纹上的表达及其与年龄相关性听力下降之间的关系。方法将健康豚鼠按照年龄分为:2周组、3月组、1年组、D-半乳糖(D-galactose,D-gal)衰老模型组;听性脑干反应(auditory brainstem response,ABR)检测不同鼠龄豚鼠的听力变化;免疫荧光技术和免疫印迹实验(Western blot)检测TMEM16A在不同鼠龄豚鼠耳蜗血管纹中的分布和表达改变。结果 ABR反应阈值随鼠龄增长呈逐渐增高趋势,D-gal组与其他3组之间的差异具有统计学意义(P0.01)。TMEM16A在不同鼠龄豚鼠耳蜗血管纹中均有表达,且表达量从2周组到1年组随鼠龄的增加呈上升趋势(P均0.05);D-gal组表达量减少,与3月组和1年组均有统计学意义(P0.05)。结论 TMEM16A在豚鼠耳蜗血管纹表达的变化可能与年龄相关性听力下降相关。
[Abstract]:Objective to investigate the expression of transmembrane protein 16A(transmembrane protein 16A on stria vascularis of guinea pigs of different ages and its relationship with age-related hearing loss. Methods healthy guinea pigs were divided into 2 weeks group, 3 months group, 1 year group with D-galactoseD-galactoseD-galactoseal model according to their age, and the auditory brainstem response (auditory brainstem response) auditory brainstem response (ABR) was used to detect the hearing changes of guinea pigs of different ages. The distribution and expression of TMEM16A in cochlear stria of guinea pigs of different ages were detected by immunofluorescence and Western blotting. Results the threshold of ABR reaction increased gradually with the aging of rats. The difference between D-gal group and other three groups was statistically significant. P0.01A. TMEM16A was expressed in cochlear stria of guinea pigs of different ages. The expression of P0. 05 and D-gal decreased from 2 weeks to 1 year group, which was significantly higher than that of 3 month group and 1 year group, P 0. 05%, P 0. 05%, P < 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%. Conclusion the expression of TMEM16A in cochlear stria may be related to age-related hearing loss.
【作者单位】：石河子大学医学院基础医学系生理学教研室新疆地方与民族高发病教育部重点实验室;
【基金】：国家自然科学基金资助项目(No.81560175,81460098,31460264)~~
【分类号】：R764.436

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