miRNA-143对人鼻咽癌细胞CNE-2Z增殖、迁移和侵袭的影响

发布时间：2018-04-24 11:56

本文选题：鼻咽癌 + miR-　；参考：《安徽医科大学学报》2017年04期

【摘要】：目的探讨miRNA-143对鼻咽癌细胞CNE-2Z增殖、迁移和侵袭的影响。方法利用荧光定量PCR检测人永生化鼻咽上皮细胞(NP69)、高分化鼻咽癌细胞(CNE-1)、低分化鼻咽癌细胞(CNE-2Z)中miRNA-143的表达水平。利用感染慢病毒的方式建立稳定过表达miRNA-143的CNE-2Z细胞,并用荧光定量PCR法进行检测。通过CCK-8法检测过表达miRNA-143对CNE-2Z细胞增殖的影响,通过Transwell迁移和侵袭试验分析过表达miRNA-143对CNE-2Z细胞迁移和侵袭的影响。结果低分化鼻咽癌细胞(CNE-2Z)中miRNA-143的表达水平显著低于CNE-1和NP69细胞(P0.01)。稳定过表达miRNA-143的CNE-2Z细胞(CNE-2Z/miR-143)中miRNA-143的表达水平显著高于CNE-2Z细胞(P0.01)和慢病毒对照组细胞(CNE-2Z/miR-NC)(P0.01)。细胞增殖能力检测显示,与CNE-2Z组和CNE-2Z/miR-NC组相比,CNE-2Z/miR-143组在72 h和96 h时细胞活力显著降低(P0.05,P0.01)。Transwell迁移和侵袭试验结果显示,CNE-2Z/miR-143组与CNE-2Z组和CNE-2Z/miR-NC组相比细胞迁移和侵袭能力显著下降(P0.01)。结论 miR-143能够抑制鼻咽癌细胞CNE-2Z的增殖、迁移和侵袭能力,提示其可能对鼻咽癌的诊断、治疗及预后评价有重要意义。
[Abstract]:Objective to investigate the effects of miRNA-143 on the proliferation, migration and invasion of nasopharyngeal carcinoma (NPC) CNE-2Z cells. Methods fluorescence quantitative PCR was used to detect the expression of miRNA-143 in human immortalized nasopharyngeal epithelial cells (NP69), well-differentiated nasopharyngeal carcinoma cells (CNE-1) and poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z). Stable CNE-2Z cells expressing miRNA-143 were established by lentivirus infection and detected by fluorescence quantitative PCR. The effects of overexpression of miRNA-143 on the proliferation of CNE-2Z cells were detected by CCK-8 assay, and the effects of overexpression of miRNA-143 on the migration and invasion of CNE-2Z cells were analyzed by Transwell migration and invasion assay. Results the expression of miRNA-143 in poorly differentiated nasopharyngeal carcinoma cells was significantly lower than that in CNE-1 and NP69 cells. The expression of miRNA-143 in CNE-2Z cells with stable expression of miRNA-143 was significantly higher than that in CNE-2Z cells (P0.01) and lentivirus control cells (P0.01). Compared with CNE-2Z group and CNE-2Z/miR-NC group, the cell viability of CNE-2Z / miR-143 group decreased significantly at 72 h and 96 h. The results of migration and invasion test showed that the migration and invasion ability of CNE-2Z / miR-143 group was significantly lower than that of CNE-2Z group and CNE-2Z/miR-NC group. Conclusion miR-143 can inhibit the proliferation, migration and invasion of nasopharyngeal carcinoma (NPC) CNE-2Z cells, suggesting that miR-143 may play an important role in the diagnosis, treatment and prognosis evaluation of nasopharyngeal carcinoma.
【作者单位】：安徽医科大学第一附属医院检验科;安徽省第二人民医院检验科;
【基金】：国家自然科学基金(编号:81302525)
【分类号】：R739.63

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