鼻阻塞对生长发育期大鼠上气道影响的形态学及计算流体力学研究

发布时间：2018-04-24 12:35

本文选题：鼻阻塞 + 大鼠　；参考：《山东大学》2017年硕士论文

【摘要】：目的本研究通过建立生长发育期鼻阻塞的Wistar大鼠模型,评价基于Micro-CT数据的上气道形态学和流体力学变化,为分析生长发育期鼻阻塞状态下上气道变化提供理论依据。材料和方法选取三周龄的雄性Wistar大鼠20只,随机分为两组:A组:对照组、B组:双侧鼻阻塞组,每组各10只。用特制阻塞器间歇性完全阻塞大鼠双侧外鼻,阻塞时间为8:00am至4:00pm,即每天连续阻塞8小时。49天后Wistar大鼠麻醉固定于仰卧位,摄取头颈部 Micro-CT(Skyscan1076:Skyscan,Antwerp,Belgium)数据。用MIMICS17.0对摄取的上气道数据进行三维重建,并利用ANSYS16.0软件对气道内流场特征进行仿真模拟。采用SPSS19.0软件进行对照组与实验组上气道三维形态及流场特征变化的配对t检验分析,检验标准设P0.05。对上气道的气流阻力(R)与上气道的形态学参数(体积V、平均横截面积Smean、气道一致性Smin/Smean)的相关关系进行皮尔森相关性检验(Pearson correlation analyze)分析。结果间歇性双侧完全鼻阻塞组的上气道体积及平均横截面积较对照组明显减小,其中气道体积及平均横截面积变化最显著的部位在口咽处,且此处的形态一致性Smin/Smean明显差于对照组。上气道流场特征的数值模拟显示,气流最大负压在喉部而气流最大压降及最高流速处均在口咽。实验组的压降和气流流速较对照组明显增大,变化最明显处也在口咽。该处的压降变化与体积、平均横截面积和形态一致性变化呈负相关关系。结论生长发育期的鼻阻塞使上气道狭窄,口咽部位最明显,通气功能明显降低。
[Abstract]:Objective to evaluate the changes of upper airway morphology and fluid mechanics based on Micro-CT data by establishing a Wistar rat model of the growth and development of nasal obstruction, and to provide a theoretical basis for the analysis of the upper airway changes during the growth and development of nasal obstruction. 20 male Wistar rats of three weeks old were randomly divided into two groups: A group: The control group, B group: bilateral nasal obstruction group, each group of 10. With the special blocker intermittently completely obstruct the rat bilateral outer nose, the blocking time is 8:00am to 4:00pm, that is, every day after 8 hours of obstruction, Wistar rats are fixed at the supine position, and the Micro-CT (Skyscan1076:Skyscan, Antwerp, Belgium) data of the head and neck are taken. MIMICS17.0 is used. The data of the upper airway was reconstructed, and the characteristics of the flow field in the airway were simulated by ANSYS16.0 software. The paired t test was used to analyze the three-dimensional shape and flow characteristics of the upper airway in the control group and the experimental group by the SPSS19.0 software, and the test standard set up the morphological parameters of the air flow resistance (R) and the upper airway of the upper gas channel by the standard P0.05.. The correlation of number (volume V, mean cross section area Smean, airway conformance Smin/Smean) was analyzed by Pearson correlation test (Pearson correlation analyze). Results the upper airway volume and average cross section area of intermittent bilateral complete nasal obstruction group were significantly smaller than those of the control group. The changes in the volume and average cross section area of the airway were the most significant. In the oropharynx, the shape consistency of the Smin/Smean is significantly worse than that in the control group. The numerical simulation of the flow field in the upper airway shows that the maximum pressure drop in the throat and the maximum flow pressure drop and the maximum flow velocity are all in the oropharynx. The pressure drop and the flow velocity of the experimental group are significantly higher than those of the control group, and the most obvious change is also in the oropharynx. There was a negative correlation between the pressure drop and the volume, the average cross section area and the shape consistency. Conclusion the nasal obstruction caused the upper airway stenosis, the most obvious oropharynx, and the ventilation function decreased.

【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R766;R782

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