不同比例激活剂对富血小板凝胶的生物学影响

发布时间：2018-04-27 08:53

本文选题：激活剂 + 富血小板凝胶　；参考：《中国现代医学杂志》2017年20期

【摘要】：目的比较不同比例激活剂对富血小板凝胶的生物学影响,研究其用于角膜基质再生的可行性。方法抽取兔全血,经二次离心法制备富血小板血浆(PRP),将PRP与激活剂按不同比例激活,制备PRP凝胶。实验分为3个实验组,分别为9∶1组(PRP∶激活剂=9∶1)、11∶1组(PRP∶激活剂=11∶1)及5∶1组(PRP∶激活剂=5∶1)。将兔角膜基质细胞与PRP凝胶共同培养,进行组织形态学观察,用细胞增殖-毒性检测试剂盒法,分别检测3个实验组PRP凝胶释放的复合生长因子促角膜基质细胞的增殖作用。对不同实验组制备的PRP凝胶与双层猪角膜基质进行黏附性观察。结果 9∶1组制备的PRP凝胶促进兔角膜基质细胞的增殖和活化作用强于其他实验组。3个实验组中,只有9∶1组制备的PRP凝胶苏木精-伊红染色法染色结果显示,呈有序的网状支架结构,能紧密黏附并固定双层猪角膜基质。结论 PRP与激活剂以9∶1比例制备的PRP凝胶促角膜基质细胞增殖作用最强,同时具有有序网状结构和良好的组织黏附性及相容性,是一种可以用于角膜基质再生的良好支架。
[Abstract]:Objective to compare the biological effects of different ratios of activators on platelet-rich gel and to study the feasibility of its application in corneal stromal regeneration. Methods the rabbit whole blood was extracted and the platelet-rich plasma was prepared by secondary centrifugation. The PRP gel was prepared by activating PRP and activator in different proportion. The experiment was divided into three groups: 9:1 group (PRP: activator 9: 1) and 5:1 group (group PRP: activator 5: 1). Rabbit corneal stromal cells were co-cultured with PRP gel to observe the histomorphology. The proliferation of corneal stromal cells was detected by PRP gel combined growth factor in three experimental groups with the method of cell proliferation-toxicity test kit. The adhesion of PRP gel prepared by different experimental groups to double layer porcine corneal stroma was observed. Results the effect of PRP gel prepared at 9:1 on the proliferation and activation of rabbit corneal stromal cells was stronger than that of other experimental groups. Of the 3 experimental groups, only the PRP gel prepared at 9:1 was stained with hematoxylin and eosin. It is an orderly reticular scaffold which can tightly adhere to and immobilize double-layer porcine corneal stroma. Conclusion the PRP gel prepared with PRP and activator at 9:1 has the strongest effect on the proliferation of corneal stromal cells and has ordered reticular structure and good tissue adhesion and compatibility. It is a good scaffold for corneal stromal regeneration.
【作者单位】：广西省柳州市工人医院眼科;暨南大学医学院眼科研究室(再生医学教育部重点实验室);
【基金】：国家自然科学基金(No:30973244)
【分类号】：R77

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