ALDH1、HIF-1α和VEGF在喉鳞状细胞癌中的表达及意义

发布时间：2018-05-06 16:16

本文选题：喉鳞状细胞癌 + ALDH　；参考：《四川大学学报(医学版)》2017年03期

【摘要】：目的研究乙醛脱氢酶1(ALDH1)、低氧诱导因子1α(HIF-1α)和血管内皮生长因子(VEGF)在喉鳞状细胞癌(LSCC)组织中的表达及意义。方法采用原位杂交实验检测120例LSCC和60例对照喉组织中ALDH1、HIF-1α和VEGF mRNA表达,应用免疫组化Elivision法检测上述因子的蛋白表达情况,统计分析各因子表达与相关临床病理特征和预后的关系。结果 ALDH1、HIF-1α和VEGF mRNA在LSCC中的阳性表达率分别为57.5%(69/120)、73.3%(88/120)和56.7%(68/120),三者在LSCC中的蛋白阳性表达率分别为55.8%(67/120)、70.8%(85/120)和55.0%(66/120),与对照组比较差异均有统计学意义(P0.05)。随着LSCC患者临床分期的增高及淋巴结转移的出现,ALDH1和VEGF蛋白的阳性表达率越高,差异有统计学意义(P0.05)。HIF-1α蛋白的阳性表达与患者的TNM分期、组织分化程度、淋巴结是否转移有关(P0.05)。三者的表达之间存在一定的正相关性(P0.05)。ALDH1、HIF-1α和VEGF三种因子中两者(或三者)联合表达阳性的患者淋巴结转移率高于只表达任一单个分子或两者均不表达(或三者不同时表达)的患者(P0.05)。三种因子表达阳性患者5年生存率均较阴性表达患者低(P均0.05),三者联合表达的患者预后更差。结论 ALDH1、HIF-1α及VEGF在LSCC中表达上调,三者的联合检测对LSCC的发生、发展、侵袭转移和判断预后有一定意义。
[Abstract]:Objective to investigate the expression and significance of aldehyde dehydrogenase (ALDH 1), hypoxia inducible factor 1 伪 (HIF-1 伪) and vascular endothelial growth factor (VEGF) in laryngeal squamous cell carcinoma (LSCC). Methods the expression of ALDH1HIF-1 伪 and VEGF mRNA in 120 cases of LSCC and 60 cases of control larynx were detected by in situ hybridization. The expression of HIF-1 伪 and VEGF mRNA were detected by immunohistochemical Elivision method. The relationship between the expression of various factors and clinicopathological features and prognosis was analyzed statistically. Results the positive expression rates of ALDH1mHIF-1 伪 and VEGF mRNA in LSCC were 57.5 and 73.33 / 120, respectively, and the positive rates of the three proteins in LSCC were 55.867 / 120 / 70.885 / 120) and 55.066 / 120, respectively. There were significant differences between the two groups (P0.05 / 120). With the increase of clinical stage and lymph node metastasis in LSCC patients, the positive expression rate of ALDH1 and VEGF protein was higher. The difference was statistically significant between the positive expression of P0.05U. HIF-1 伪 protein and TNM stage, tissue differentiation, lymph node metastasis or not. There was a positive correlation between the expression of the three factors and the lymph node metastasis rate of patients with positive expression of HIF-1 伪 and VEGF was higher than that of only one single molecule or neither of them (or different from each other). P0. 05% of the patients were treated with P0. 05. The 5-year survival rate of the patients with positive expression of the three factors was lower than that of the patients with negative expression (P < 0.05), and the prognosis of the patients with the combined expression of the three factors was worse. Conclusion the expression of ALDH1HIF-1 伪 and VEGF in LSCC is up-regulated. The combined detection of ALDH1a and HIF-1 伪 is of significance in the occurrence, development, invasion, metastasis and prognosis of LSCC.
【作者单位】：蚌埠医学院第一附属医院临床病理科蚌埠医学院病理学教研室;蚌埠医学院第一附属医院肿瘤外科;
【基金】：国家自然科学基金(No.81173386) 安徽省高等学校自然科学研究一般项目(No.KJ2015B045by);安徽省高等学校自然科学研究一般项目(No.KJ2015B040by)资助
【分类号】：R739.65

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