原花青素对氧化应激人眼小梁网细胞凋亡及细胞色素C释放的影响

发布时间：2018-05-09 05:03

本文选题：原花青素 + 小梁网细胞　；参考：《眼科新进展》2017年10期

【摘要】：目的研究原花青素对H2O2诱导人眼小梁网细胞(human trabecular meshwork cells,HTMC)凋亡及细胞色素C释放的影响。方法将HTMC进行传代后随机分为5组。未处理组:正常培养的HTMC;对照组:正常培养的HTMC+H_2O_2(500μmol·L~(-1)处理1 h);3个浓度的原花青素组:正常培养的HTMC+H_2O_2(500μmol·L~(-1)处理1 h)+原花青素(浓度分别为0.02 g·L~(-1)、0.05 g·L~(-1)、0.10 g·L~(-1))。使用Annexin V-FITC检测细胞凋亡情况,Western blot检测HTMC胞浆细胞色素C含量。结果与未处理组相比,对照组及各原花青素组细胞凋亡率均升高,差异均有统计学意义(均为P0.01)。与对照组相比,各原花青素组细胞凋亡率均降低,差异均有统计学意义(均为P0.01);不同浓度原花青素组间随原花青素浓度增加细胞凋亡率下降,各原花青素组间差异均有统计学意义(均为P0.01)。与未处理组相比,对照组以及0.02 g·L~(-1)和0.05 g·L~(-1)原花青素组细胞色素C的释放均增加,差异均有统计学意义(均为P0.01);而0.10 g·L~(-1)原花青素组与未处理组相比,差异无统计学意义(P0.05)。与对照组相比,各原花青素组细胞色素C释放均减少,差异均有统计学意义(均为P0.01)。不同浓度原花青素组间随原花青素浓度增加细胞色素C的释放减少,各组间差异均有统计学意义(均为P0.01)。结论外源性原花青素可以降低氧化应激HTMC的凋亡率,减少细胞色素C的释放,有较强的抗氧化作用,并在一定浓度范围内随着浓度的增加其抗氧化作用逐渐增强。
[Abstract]:Objective to study the effect of procyanidins on apoptosis and cytochrome C release in human trabecular meshwork cells (HTMC) induced by H2O2. Methods HTMC were randomly divided into 5 groups after passage. Untreated group: normal culture HTMCs; control group: normal cultured HTMC H_2O_2(500 渭 mol L ~ (1) treated for 1 hour; 3 concentration of proanthocyanidins group: normal culture HTMC H_2O_2(500 渭 mol L ~ (-1) for 1 h) procyanidins (0.02g / L ~ (-1) (0.02g / L ~ (-1) ~ (-0.10) g / L ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1). Apoptosis was detected by Annexin V-FITC and cytochrome C in cytoplasm of HTMC was detected by Western blot. Results compared with the untreated group, the apoptotic rate of the control group and the proanthocyanidins group were significantly higher than that of the untreated group (P 0.01). Compared with the control group, the apoptotic rate of each proanthocyanidin group was significantly lower than that of the control group (P 0.01), and the apoptosis rate decreased with the increase of proanthocyanidin concentration among different concentrations of proanthocyanidins. The difference of proanthocyanidin group was statistically significant (P 0.01). Compared with untreated group, the release of cytochrome C in the control group and 0.02 g / L ~ (-1) and 0.05 g / L ~ (-1) proanthocyanidins group was significantly higher than that in the untreated group (all P 0.01 ~ (-1); 0.10 g / L ~ (-1) there was no significant difference between the procyanidin group and the untreated group (P 0.05). Compared with the control group, the release of cytochrome C in each procyanidin group was significantly lower than that in the control group (P 0.01). With the increase of proanthocyanidin concentration, the release of cytochrome C decreased in different concentrations of proanthocyanidins, and the difference was statistically significant (P 0.01). Conclusion exogenous proanthocyanidins can reduce the apoptosis rate of oxidative stress HTMC and reduce the release of cytochrome C.
【作者单位】：南昌大学第二附属医院眼科;
【分类号】：R775

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