miR-34b在老年鼻咽癌组织中表达及对细胞增殖、侵袭的影响
本文选题:鼻咽癌 + miR-b ; 参考:《中国老年学杂志》2017年07期
【摘要】:目的探讨miR-34b在老年鼻咽癌组织中表达及对细胞增殖、侵袭的影响。方法选取初治原发性老年鼻咽癌患者83例,正常鼻咽黏膜组织40例作为对照组,培养人鼻咽癌CNE-2细胞株,根据转染物不同将细胞分为miR-34b转染组、阴性对照组和空白对照组,利用实时荧光定量PCR技术检测老年鼻咽癌及对照组组织中miR-34b表达以及不同转染组细胞中miR-34b表达,MTT比色法检测不同转染组细胞增殖能力,检测细胞黏附能力,Transwell法检测不同转染组细胞侵袭能力。结果老年鼻咽癌组织中miR-34b相对表达量为(0.64±0.09),显著低于对照组的(0.94±0.12),差异有统计学意义(t=14.410,P0.001);miR-34b在老年鼻咽癌组织中相对表达量与临床分期、T分期、N分期和淋巴结转移有关(P0.001);miR-34b转染组细胞中miR-34b相对表达量为(0.85±0.10),显著高于阴性对照组和空白对照组,分别为(0.52±0.08)和(0.50±0.07),差异有统计学意义(F=243.329,P0.001);与阴性对照组和空白对照组相比,miR-34b转染组细胞增殖能力显著降低(P0.05);miR-34b转染组黏附细胞数显著高于阴性对照组和空白对照组,而侵袭细胞数显著低于阴性对照组和空白对照组(P0.001)。结论 miR-34b在老年鼻咽癌组织中呈低表达,上调miR-34b表达可减少细胞增殖,增加细胞黏附能力,抑制细胞侵袭能力,有望成为提高鼻咽癌对放射治疗敏感性的目的基因。
[Abstract]:Objective to investigate the expression of miR-34b in the tissues of elderly nasopharyngeal carcinoma (NPC) and the effect on cell proliferation and invasion. Methods 83 patients with primary nasopharyngeal carcinoma and 40 normal nasopharyngeal mucosa tissues were selected as control group, and the human nasopharyngeal carcinoma CNE-2 cell lines were cultured. The cells were divided into miR-34b transfection group, negative control group and blank control group according to the different transfection. The expression of miR-34b in the elderly nasopharyngeal carcinoma and the control group and the expression of miR-34b in the different transfected groups were detected by real time fluorescence quantitative PCR. The cell proliferation ability of different transfected groups was detected by MTT colorimetric assay and the cell adhesion ability was detected by the Transwell method. The results of m in the old nasopharyngeal carcinoma tissues were determined by Transwell. The relative expression of iR-34b was (0.64 + 0.09), significantly lower than that of the control group (0.94 + 0.12), and the difference was statistically significant (t=14.410, P0.001). The relative expression of miR-34b in the old nasopharyngeal carcinoma tissues was related to the clinical stage, T stage, N staging and lymph node metastasis (P0.001), and the relative expression of miR-34b in the cells of miR-34b transfected group was (0.85 + 0.10). Compared with negative control group and blank control group (0.52 + 0.08) and (0.50 + 0.07), the difference was statistically significant (F=243.329, P0.001). Compared with the negative control group and the blank control group, the cell proliferation ability of miR-34b transfected group was significantly decreased (P0.05), and the number of adhesion cells in miR-34b transfer group was significantly higher than that of the negative control group and the blank control group, and the number of cells in the miR-34b transfer group was significantly higher than that in the negative control group and the blank control group. The number of invasive cells was significantly lower than that of the negative control group and the blank control group (P0.001). Conclusion miR-34b is low expression in the elderly nasopharyngeal carcinoma tissue. Up regulation of miR-34b expression can reduce cell proliferation, increase cell adhesion ability and inhibit cell invasiveness. It is expected to be the target gene to improve the sensitivity of nasopharyngeal carcinoma to radiotherapy.
【作者单位】: 中航工业成都363医院;
【基金】:四川省卫计委基本医学科研项目(No.110103)
【分类号】:R739.63
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