促性腺激素释放激素抑制人鼻咽癌CNE2细胞迁移和侵袭能力的研究

发布时间：2018-05-11 13:15

本文选题：促性腺激素释放激素 + 鼻咽肿瘤　；参考：《临床耳鼻咽喉头颈外科杂志》2017年11期

【摘要】：目的:探讨促性腺激素释放激素(GnRH)对人鼻咽癌CNE2细胞活力、凋亡、迁移和侵袭能力的影响。方法:收集鼻咽癌和慢性鼻咽炎症的组织标本,采用免疫组织化学染色、qRT-PCR分析检测标本中GnRH阳性细胞、GnRH mRNA的表达。体外培养人鼻咽癌CNE2细胞,永生化鼻咽上皮细胞株NP69,采用免疫组织化学染色,qRT-PCR分析检测细胞中GnRH阳性细胞、GnRH mRNA的表达。以不同浓度0(空白对照组)、10~(-2)、10~(-1)、10~0nmol/L GnRH处理CNE2细胞,采用CCK-8、流式细胞术、划痕修复实验、Transwell小室体外侵袭实验观察GnRH对CNE2细胞活力、凋亡、迁移和侵袭能力的影响。结果:鼻咽癌组织中GnRH阳性细胞、GnRH mRNA表达明显低于慢性鼻咽炎症组织(P0.05)。CNE2细胞中GnRH阳性细胞、GnRH mRNA表达明显低于NP69(P0.05)。与空白对照组比较,GnRH显著抑制CNE2细胞活力、凋亡、迁移和侵袭能力(P0.05或P0.01)。结论:GnRH显著抑制CNE2细胞活力、凋亡、迁移和侵袭能力。
[Abstract]:Objective: To investigate the effect of gonadotropin releasing hormone (GnRH) on the activity, apoptosis, migration and invasion of human nasopharyngeal carcinoma (NPC) CNE2 cells. Methods: the tissue specimens of nasopharyngeal carcinoma and chronic nasopharyngitis were collected, immunohistochemical staining was used, and the expression of GnRH positive cells in the specimens, the expression of GnRH mRNA in the specimens were detected by qRT-PCR analysis. The human nasopharyngeal carcinoma CNE2 was cultured in vitro CNE2. Cells, immortalized nasopharyngeal epithelial cell strain NP69, using immunohistochemical staining, and qRT-PCR analysis to detect the expression of GnRH positive cells and GnRH mRNA in cells. The CNE2 cells were treated with different concentrations of 0 (blank control group), 10~ (-2), 10~ (-1), 10~0nmol/L GnRH. The effects of GnRH on the activity, apoptosis, migration and invasion of CNE2 cells were observed. Results: the expression of GnRH positive cells in nasopharyngeal carcinoma tissues was significantly lower than that of GnRH positive cells in the chronic nasopharyngitis (P0.05).CNE2 cells, and the expression of GnRH mRNA was significantly lower than NP69 (P0.05). Conclusion: GnRH significantly inhibited the viability, apoptosis, migration and invasion of CNE2 cells in P0.05 cells, P0.01.

【作者单位】：牡丹江医学院附属红旗医院耳鼻咽喉头颈外科;牡丹江医学院附属红旗医院内分泌科;
【分类号】：R739.63

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