miR-122a抑制人喉癌细胞株Hep2细胞增殖的研究

发布时间：2018-05-27 11:03

本文选题：喉肿瘤 + miR-a　；参考：《中国免疫学杂志》2017年03期

【摘要】：目的:研究miR-122a对人喉癌细胞株Hep2细胞增殖、细胞周期以及相关蛋白表达量的影响。方法:人喉癌细胞株Hep2细胞分别转染miR-122a寡聚核苷酸(A组)和miR-122a inhibitor(阻遏物)(B组),同时设立阻遏物阴性对照(inhibitor negative control,miR-NC inhibitor)(C组)和空白对照(D组)。用RT-PCR、MTT法、流式细胞仪和Western blot技术评价各组细胞增殖和细胞周期的生物学特征。结果:转染miR-122a寡聚核苷酸后,Hep2细胞中miR-122a表达显著增加。同D组相比,A组细胞增殖水平明显受到抑制,miR-122a寡聚核苷酸能够有效诱导Hep-2细胞周期阻滞在G1/G0期,A组细胞分裂周期蛋白42表达水平明显下调,细胞周期调控因子蛋白CDK4及细胞周期素D1蛋白表达水平明显降低。结论:miR-122a寡聚核苷酸能够显著抑制喉癌细胞Hep2的增殖,miR-122a是潜在的人喉癌细胞基因治疗的候选靶点。
[Abstract]:Objective: To study the effect of miR-122a on the proliferation, cell cycle and the expression of related protein in human laryngocarcinoma cell line Hep2. Methods: Hep2 cells of human larynx cell lines were transfected to miR-122a oligonucleotides (group A) and miR-122a inhibitor (repressor) (B group), and the negative control of repressor was set up (inhibitor negative control, miR-NC) R) (group C) and blank control (group D). The biological characteristics of cell proliferation and cell cycle were evaluated by RT-PCR, MTT, flow cytometry and Western blot. Results: the expression of miR-122a expression in Hep2 cells increased significantly after transfection of miR-122a oligonucleotides. Compared with D group, the proliferation level of A group cells was obviously inhibited, oligonucleotides were found. It can effectively induce Hep-2 cell cycle arrest in G1/G0 phase, the expression level of cell division cyclin 42 in group A is obviously down, cell cycle regulator protein CDK4 and cyclin D1 protein expression level decrease obviously. Conclusion: miR-122a oligodeoxynucleotides can significantly inhibit the proliferation of Hep2 in laryngeal cancer cells, miR-122a is a potential human Laryngic cancer thin. Candidate targets for cell gene therapy.
【作者单位】：常熟市第二人民医院耳鼻咽喉科;苏州大学附属第一医院耳鼻咽喉科;
【分类号】：R739.65

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