鞣花酸抗鼻咽癌CNE-2细胞的作用及分子机制初探

发布时间：2018-05-29 13:47

本文选题：鞣花酸 + 鼻咽癌　；参考：《桂林医学院》2012年硕士论文

【摘要】：目的：研究五倍子提取物鞣花酸对鼻咽癌CNE-2细胞增殖和凋亡的影响,探讨鞣花酸抗鼻咽癌的生物学作用及其可能的机制。方法：培养鼻咽癌CNE-2细胞24h后,设为4组实验组,3组药物组分别加入(2、4、6mg/1)的鞣花酸干预,对照组(即0mg/1)不干预。1.鞣花酸干预48h后,显微镜下与对照组比较观察鞣花酸对鼻咽癌CNE-2细胞的形态的变化。2.鞣花酸干预48h后,Hoechst33258法染色,与对照组比较观察鞣花酸对鼻咽癌CNE-2细胞凋亡的作用。3.分别于鞣花酸干预24h、48h、72h后,MTT实验分析,与对照组相比鞣花酸对鼻咽癌CNE-2细胞增殖的影响。4.鞣花酸干预48h后,流式细胞仪分析,与对照组比较鞣花酸对鼻咽癌CNE-2细胞凋亡率的影响。5.鞣花酸干预48h后,Western-blot检测,与对照组相比鞣花酸对鼻咽癌CNE-2细胞中COX-2、 NF-κB的表达的影响。结果：1.浓度为(0、2、4、6mg/1)的鞣花酸干预鼻咽癌CNE-2细胞后,随药物浓度的增大,漂浮的死亡细胞数量随之增加。鼻咽癌CNE-2活细胞数量随药物浓度的增大而明显减少。2. Hoechst33258染色后,随药物浓度的增大,鼻咽癌CNE-2染色细胞减少,而细胞核浓缩、核破裂的数量随药物浓度的增大而增多。3.MTT法检测,随药物浓度的增加,鼻咽癌CNE-2细胞明显的生长抑制现象随之增加,呈浓度依赖性。3.流式细胞术结果显示：鞣花酸处理鼻咽癌CNE-2细胞48h后,药物组(2、4、6mg/l)凋亡率分别为：(19.17%±0.91%、38.44%±3.13%、61.74%±4.28%),与对照组(0.21%±0.08%)相比,药物组凋亡现象明显(P0.05),药物组之间两两比较差异明显,药物浓度越高,凋亡越明显(P0.05)。3. Western-blot检测结果：各药物组COX-2、NF-κB与对照组的比较均表达降低,浓度越高,表达越低。结论：鞣花酸能抑制鼻咽癌CNE-2细胞的增殖,诱导其凋亡；其机制可能与抑制COX-2和NF-κB的表达有关。
[Abstract]:Objective: To study the effect of tannic acid of Chinese gall extract on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cells, and to explore the biological effect of tannic acid on nasopharyngeal carcinoma and its possible mechanism.
Methods: after the culture of nasopharyngeal carcinoma CNE-2 cells 24h, 4 groups of experimental groups were set up. The 3 groups were added (2,4,6mg/1) to the tannic acid intervention, the control group (0mg/1) did not interfere with.1. tannic acid intervention 48h, the morphological changes of tannic acid to nasopharyngeal carcinoma CNE-2 cells were compared with the control group under microscope and.2. tannic acid intervention 48h, Hoechst33258 staining after 48h, Hoechst33258 staining. Color, the effect of tannic acid on the apoptosis of nasopharyngeal carcinoma CNE-2 cells was compared with the control group.3.. The effect of tannic acid on 24h, 48h, 72h, MTT experimental analysis. Compared with the control group, the effects of tannic acid on the proliferation of nasopharyngeal carcinoma CNE-2 cells were compared with the control group. After the intervention 48h, the flow cytometer was analyzed by the flow cytometer, and the tannic acid was compared with the control group for the CNE-2 cell of nasopharyngeal carcinoma. Effects of.5. after ellagic acid intervention on 48h, Western-blot detected the effect of ellagic acid on the expression of COX-2 and NF- kappa B in CNE-2 cells compared with the control group.
Results: after the concentration of tannic acid (1. 0,2,4,6mg/1) interfered with nasopharyngeal carcinoma CNE-2 cells, the number of floating dead cells increased with the increase of drug concentration. The number of CNE-2 living cells in nasopharyngeal carcinoma decreased significantly with the increase of drug concentration and decreased with.2. Hoechst33258 staining. With the increase of drug concentration, the CNE-2 staining cells of nasopharyngeal carcinoma decreased, and the number of CNE-2 stained cells in nasopharyngeal carcinoma decreased. The number of nuclear rupture and nuclear rupture increased with the increase of drug concentration and increased by.3.MTT method. With the increase of drug concentration, the obvious growth inhibition of nasopharyngeal carcinoma CNE-2 cells increased. The result of concentration dependent.3. flow cytometry showed that after tannic acid treatment of nasopharyngeal carcinoma CNE-2 cell 48h, the apoptosis rate of drug group (2,4,6mg/l) was respectively: (19.17% + 0.91%, 38.44% + 3.13%, 61.74% + 4.28%), compared with the control group (0.21% + 0.08%), the drug group had obvious apoptotic phenomenon (P0.05), and the difference was obvious between the drug group 22, the higher the drug concentration, the more obvious apoptosis (P0.05).3. Western-blot detection results: COX-2, NF- kappa B in each drug group decreased with the control group, the higher the concentration, the higher the concentration, The lower the expression.
Conclusion: ellagic acid can inhibit the proliferation and induce apoptosis of nasopharyngeal carcinoma CNE-2 cells, and its mechanism may be related to inhibiting the expression of COX-2 and NF- kappa B.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63

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