Gal-1,ANXA2和PRPH在SD大鼠耳蜗核的表达

发布时间：2018-05-29 22:08

  本文选题：中枢听觉处理障碍 + CN　； 参考：《南京医科大学学报(自然科学版)》2017年05期

【摘要】：目的 :采用同位素相对标记和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQi)技术分析耳蜗核(cochlear nucleus,CN)区域特异性膜蛋白。方法 :出生60 d的雄性SD大鼠分为4组,分别是CN、下丘(inferior colliculus,IC)、上橄榄核(superior oliver complex,SOC)、大脑其余部位(Rest)。提取这4个部位组织的细胞膜蛋白,再采用iTRAQi技术检测出CN区域特异性蛋白。通过Uniprot检索这些蛋白的功能和参与的生物代谢过程。结果:本实验最终得到17种CN区域特异性膜蛋白。对CN区域蛋白进行基因本体(gene ontology,GO)分析发现,这些蛋白主要参与生物调节与发育。UNIPROT检索发现3种蛋白与神经系统功能相关,即半乳糖凝集素-1(galectin-1,Gal-1)、膜联蛋白A2(annexin-A2,AXNA2)和外周蛋白(peripherin,PRPH)。Gal-1与神经生长发育相关;AXNA2参与多种生物调节过程;PRPH与神经退行性病变相关。其中,Gal-1可促进轴突生长、调节突触可塑性且具有神经保护作用;AXNA2可能参与了神经递质释放过程,且有神经营养作用;PRPH在高表达时具有神经毒性,和神经丝蛋白共同参与神经退行性病变。结论:Gal-1、ANXA2和PRPH在耳蜗核中高度表达,可能与中枢信息处理相关,这为进一步研究中枢听觉处理障碍发病机制提供了一定的理论基础。
[Abstract]:Objective: to analyze cochlear nucleus specific membrane protein by using isotopic relative labeling and absolute quantitative tags for relative and absolute quantitative analysis. Methods: male Sprague-Dawley rats (60 days old) were divided into 4 groups: CNS, inferior colliculus, superior olivary nucleus superior oliver complex oliver, and rest of brain. Cell membrane proteins were extracted from the four tissues and CN region-specific proteins were detected by iTRAQi. The function of these proteins and their involvement in biological metabolic processes were retrieved by Uniprot. Results: 17 CN domain specific membrane proteins were obtained. The analysis of CN domain proteins revealed that these proteins were mainly involved in biological regulation and development. UNIPROT search revealed that three proteins were related to nervous system function. That is, galactose-galectin-1 (Gal-1), annexin-A2annexin-A2AXNA2) and peripheral protein (PRPH.Gal-1) are involved in various biological regulation processes, PRPH and neurodegenerative diseases. Gal-1 can promote axon growth, regulate synaptic plasticity and have neuroprotective effect. AXNA2 may be involved in the release of neurotransmitters. And neurofilament proteins are involved in neurodegenerative lesions. Conclusion the high expression of ANXA2 and PRPH in the cochlear nucleus may be related to the central information processing, which provides a theoretical basis for the further study of the pathogenesis of central auditory processing disorders.
【作者单位】： 南京医科大学第一附属医院耳鼻咽喉科WHO预防聋和听力减退合作中心;
【基金】：[基金项目]江苏省“科教兴卫工程”医学重点人才(RC2011073)
【分类号】：R764.4
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本文编号：1952616