人参皂苷RG3对脉络膜新生血管的抑制作用

发布时间：2018-06-03 10:14

本文选题：年龄相关性黄斑变性 + 脉络膜新生血管　；参考：《眼科新进展》2017年10期

【摘要】：目的分别从体外实验和体内实验两个方面探讨人参皂苷RG3对脉络膜新生血管的抑制作用。方法体外实验:MTT检测人参皂苷RG3对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)增殖的抑制作用,HUVEC分为正常组(含体积分数10%胎牛血清的DMEM/F~(-1)2培养基)、对照组(含2 g·L~(-1)DMSO)和12.5μmol·L~(-1)、25.0μmol·L~(-1)、50.0μmol·L~(-1)、100.0μmol·L~(-1)人参皂苷RG3给药组,给药处理6 h后测定其吸光度值。HUVEC分为对照组(含2 g·L~(-1)DMSO)和100.0μmol·L~(-1)人参皂苷RG3组,小管形成实验检测人参皂苷RG3对小管形成的抑制作用,Western blotting检测人参皂苷RG3对VEGF蛋白表达的影响。体内实验:雄性C57BL/6J小鼠20只,随机分为对照小鼠组和人参皂苷RG3小鼠组,皮下注射预处理给药2周,半导体激光造模,于光凝后第1天和第21天行荧光素眼底血管造影检查。结果 MTT结果显示,正常组、对照组、12.5μmol·L~(-1)、25.0μmol·L~(-1)、50.0μmol·L~(-1)、100.0μmol·L~(-1)人参皂苷RG3给药组的吸光度值分别为0.43±0.17、0.43±0.05、0.33±0.02、0.24±0.02、0.18±0.01、0.15±0.01,对照组与正常组相比差异无统计学意义(P0.05),其余各组与对照组相比差异均有统计学意义(均为P0.05)。小管形成实验结果显示,对照组与100.0μmol·L~(-1)人参皂苷RG3组小管形成数目分别为(72.5±5.56)个和(11.33±3.71)个,差异有统计学意义(P0.01)。Western blotting结果显示,100.0μmol·L~(-1)人参皂苷RG3组的VEGF蛋白相对表达量(0.14±0.01)明显低于对照组(0.46±0.01),差异有统计学意义(P0.01)。体内实验荧光素眼底血管造影结果显示,人参皂苷RG3小鼠组的荧光渗漏面积明显低于对照小鼠组。结论人参皂苷RG3可以抑制脉络膜新生血管的形成。
[Abstract]:Objective to investigate the inhibitory effect of ginsenoside RG3 on choroidal neovascularization in vitro and in vivo. Methods the inhibitory effect of ginsenoside RG3 on the proliferation of human umbilical vein endothelial cells (HUVECs) in vitro was assayed. RG3 was divided into three groups: normal group (DMEM/F~(-1)2 medium containing 10% fetal bovine serum), control group (containing 2 g / L) and 12.5 渭 mol / L ~ (25. 0) 渭 mol / L ~ (-1) ~ (50.0) 渭 mol / L ~ (10) mol / L ~ (-1) ginsenoside RG3 group. After 6 hours of administration, the absorbency value of ginsenoside RG3 was measured. HUVECs were divided into two groups: control group (containing 2 g mol) and ginsenoside RG3 group (100.0 渭 mol / L). The inhibition of ginsenoside RG3 on tubule formation was detected by tubulogenesis assay and the effect of ginsenoside RG3 on VEGF protein expression was detected by Western blotting. In vivo experiment: 20 male C57BL/6J mice were randomly divided into two groups: control group and ginsenoside RG3 group. The mice were pretreated by subcutaneous injection for 2 weeks, then the model was made by semiconductor laser, and the fundus fluorescein angiography was performed on the 1st and 21st day after photocoagulation. Results the results of MTT showed that the absorbance values of ginsenoside RG3 group were 0.43 卤0.170.43 卤0.050.33 卤0.020.24 卤0.02U 0.18 卤0.01U 卤0.15 卤0.01.There was no significant difference between the control group and the control group (P 0.05), and there was no significant difference between the control group and the normal group (P 0.05). The absorbance of ginsenoside RG3 group was 0.43 卤0.170.43 卤0.050.33 卤0.020.24 卤0.02U 0.18 卤0.01 卤0.15 卤0.01.There was no significant difference between the control group and the normal group (P 0.05). The results of tubulogenesis experiment showed that the number of tubules in control group and 100.0 渭 mol RG3 group was 72.5 卤5.56 and 11.33 卤3.71, respectively. The relative expression of VEGF protein in the ginsenoside RG3 group (0.14 卤0.01) was significantly lower than that in the control group (0.46 卤0.01), and the difference was statistically significant (P 0.01). In vivo fluorescein fundus angiography showed that the fluorescence leakage area of ginsenoside RG3 mice group was significantly lower than that of control group. Conclusion ginsenoside RG3 can inhibit choroidal neovascularization.
【作者单位】：锦州医科大学;
【基金】：辽宁省科学技术基金[面上项目(原优秀人才培育)](编号:201602298) 辽宁省科技厅-锦州医科大学联合基金项目(编号:20170540382)~~
【分类号】：R774.5

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