N-乙酰半胱氨酸和曲尼司特对糖尿病视网膜病变的治疗作用与机制研究

发布时间：2018-06-10 00:23

本文选题：N-乙酰半胱氨酸 + 曲尼司特　；参考：《辽宁医学院》2012年硕士论文

【摘要】：目的观察N-乙酰半胱氨酸与曲尼司特联合使用对糖尿病大鼠血清氧化应激指标的影响，并检测视网膜TNF-α和NF-κB以及视网膜血管消化铺片形态，评估其对糖尿病视网膜病变的保护作用。方法取健康SD大鼠60只，雌雄各半，随机取48只大鼠用链脲佐菌素建立糖尿病大鼠模型,随机分为糖尿病模型（DM）组，N-乙酰半胱氨酸（NAC）组，曲尼司特（TNL）组，联合用药（COM）组，余12只作为空白对照（NOR）组。常规饲养12周观察视网膜血管消化铺片形态，并行高倍视野下血管内皮细胞与周细胞计数。测定大鼠血清GSH与SOD，，免疫组化检测视网膜TNF-α，NF-κB的表达。结果 1、一般状态：饲养期间，NOR组大鼠体重明显不断增加，血糖维持正常；其余组体重均增长缓慢，实验结束时多数体重较饲养前减轻，血糖与NOR组相比始终在较高水平（P0.01）。 2、免疫组化：NOR组视网膜间少量TNF-α与NF-κB表达，与NOR组相比，各模型组TNF-α与NF-κB均呈较高表达（P0.01），DM组升高为著；与 DM组相比，用药组TNF-α与NF-κB表达均下降（P0.05），以COM组为著。 3、SOD与GSH含量：与NOR组相比，糖尿病模型及用药组大鼠GSH与SOD均不同程度降低，其中，DM与TNL组(P0.01)较NAC组和COM组（P0.05）降低显著，与DM组相比，NAC与COM组GSH和SOD含量较高(P0.05)，TNL组与DM组无明显差异（P0.05）。 4、视网膜血管形态：NOR组大鼠视网膜血管网形态完整，毛细血管径均一；管壁周边的周细胞体积稍小，染色深；管腔中央的内皮细胞大，染色浅。DM组血管走行迂曲，欠规则，直径不均一，可见无细胞毛细血管，周细胞数目减少（P0.01）。各治疗组均有一定改善，COM组治疗作用较明显结论 NAC与TNL联合使用对糖尿病视网膜病变有明显保护作用，其作用机制与拮抗自由基、炎症反应等有关。
[Abstract]:Objective to observe the effect of combined use of N-acetylcysteine and tranilast on serum oxidative stress in diabetic rats, and to detect retinal TNF- 伪, NF- 魏 B and retinal vascular digestibility paving morphology. Methods Sixty healthy SD rats, half male and female, were randomly selected to establish diabetic rat model with streptozotocin (STZ). The rats were randomly divided into two groups: N- acetylcysteine NACgroup, tranilast TNL group and combined drug control group. The remaining 12 rats were used as blank control group. The morphology of retinal vascular digestion and preparation was observed at 12 weeks after conventional feeding and the vascular endothelial cells and pericytes were counted under high power field of vision. Serum GSH and SOD were measured, and the expression of TNF- 伪 -NF- 魏 B in retina was detected by immunohistochemistry. Results 1. General state: during feeding period, the weight of rats in the nor group increased significantly, the blood glucose maintained normal, and the weight of the other groups increased slowly, and the expression of TNF- 伪 and NF- 魏 B in the retina was detected by immunohistochemistry. At the end of the experiment, most of the body weight was lighter than that before feeding, blood glucose was always at a higher level than nor group (P 0.01). A small amount of TNF- 伪 and NF- 魏 B were expressed in the retina of the small amount of TNF- 伪 and NF- 魏 B in the immunohistochemistry group. Compared with nor group, the expression of TNF- 伪 and NF- 魏 B in each model group was higher than that in the control group (P0.01DM). Compared with the DM group, the expression of TNF- 伪 and NF- 魏 B in the treated group were all decreased (P 0.05), and the contents of SOD and GSH in the com group were decreased. Compared with nor group, the GSH and SOD in the diabetic model group and the drug treated group were decreased in varying degrees, and the levels of GSH and SOD in the DM and TNL groups were significantly lower than those in the NAC group and the com group (P0.05), while the expression of TNF- 伪 and NF- 魏 B in the treated group was significantly lower than that in the control group (P 0.05). Compared with DM group, the content of GSH and SOD in NAC and com group was higher than that in DM group. There was no significant difference between TNL group and DM group. The endothelial cells in the center of the lumen were large, and the blood vessels in the group of light dyed. DM were tortuous, irregular and uneven in diameter. There was no capillary and the number of pericytes decreased (P 0.01). Conclusion NAC combined with TNL has obvious protective effect on diabetic retinopathy, and its mechanism is related to antagonistic free radical and inflammatory reaction.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1

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