VEGFC及VEGFA基因多态性与维吾尔族人群2型糖尿病性视网膜病变的相关性研究

发布时间：2018-06-16 07:58

本文选题：糖尿病性视网膜病变 + VEGFC　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:探讨血管内皮生长因子(VEGFC及VEGFA)基因多态性与新疆维吾尔族人群糖尿病性视网膜病变(DR)的关系。方法:333例无糖尿病(NC)患者、164例无糖尿病性视网膜病变(NDR)的糖尿病患者和66例非增殖期糖尿病性视网膜病变(NPDR)患者、72例增殖期糖尿病性视网膜病变(PDR)患者;应用im LDRTM基因分型技术对3个VEGFC基因位点(rs17697419,rs17697515,rs2333526)和5个VEGFA基因位点(rs3025021,rs10434,rs833061,rs2010963,rs699947)进行基因分型。结果:VEGFC和VEGFC基因中的多个单核苷酸位点多态性(single nucleotide polymorphisms,SNPs)与PDR相关。rs17697419位点的G/A基因型(P=0.019)和A等位基因(p=0.021),rs2010963的C/C基因型(p=0.017)和C等位基因(p=0.039),rs699947位点的A/A基因型(P=0.028)和A等位基因(P=0.041),rs3025021的T/T基因型(P=0.019)和T等位基因(P=0.038),rs833061的C/C基因型(P=0.028)和C等位基因(P=0.021)与PDR相关。使用Haploview软件进行单倍型分析,VEGFA位点的5个SNP位点组成频率1%的单倍型总共11种;在VEGFC位点的3个SNP位点组成频率1%的单倍型有3种。单倍型ACG(38.3%),CTC(32.7%)和CTG(28.8)是从VEGFC获得的三个最多的单倍型。单倍型GCC(93.7%)是VEGFA最多的单倍型。结论:VEGFC与VEGFA单核苷酸多态性或许与PDR的发生相关。
[Abstract]:Objective: to investigate the relationship between the polymorphism of vascular endothelial growth factor (VEGF) VEGFC and VEGF gene and diabetic retinopathy (DRV) in Xinjiang Uygur population. Methods Sixty-four patients with nondiabetic retinopathy (NDR) and 66 patients with nonproliferative diabetic retinopathy (NPDRR) without diabetic retinopathy were enrolled in this study. 72 patients with proliferative diabetic retinopathy (PDR) were included in the study. Three VEGFC loci, rs17697515, rs2333526) and five VEGFA loci, rs3025021, rs104334, rs833061, rs2010963rs699947, were genotyped by im LDRTM genotyping. Results multiple single nucleotide polymorphisms (SNPs) in the 1: VEGFC and VEGFC genes were associated with PDR.rs17697419 (P / 0.019) and A allele (p0.021 / rs2010963 / C / p0.017) and C allele (p0.039 / rs699947 / Ar / A / P0.028), respectively. The T / T genotype (P0. 019), the T allele (P0. 038) of rs833061 (P0. 028) and the C allele (P0. 021) of rs3025021) were associated with PDR. Haploview software was used to analyze the haplotypes of 5 SNP loci in VEGFA loci. There were 11 haplotypes with 1% SNP frequencies and 3 haplotypes with 1% SNP frequencies in VEGFC loci. Haplotype ACG 38.3) and CTG 28. 8) are the three most abundant haplotypes obtained from VEGFC. Haplotype GCC 93. 7) is the most haplotype of VEGFA. Conclusion the single nucleotide polymorphisms of VEGFC and VEGFA may be associated with the occurrence of PDR.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.2;R774.1

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