基于紫外光辐照处理的疏水性人工晶状体亲水化表面改性

发布时间：2018-06-16 20:34

本文选题：紫外光辐照 + 人工晶状体　；参考：《浙江大学》2015年博士论文

【摘要】：目的：利用先进的紫外光辐照技术接枝亲水性单体,实现疏水性人工晶状体(IOL)前表面亲水化改性,保留后表面的疏水特性,探讨一种能够提高IOL生物相容性的简单、经济、高效的方法,以利于进一步的工业化生产。方法：采用化学、物理两种方法预处理疏水性丙烯酸酯人工晶体以实现活性引发,采用紫外光辐照表面改性技术接枝亲水性单体2-甲基丙烯酰氧基乙基磷酰胆碱(MPC)修饰疏水性聚丙烯酸酯IOL前表面。应用静态水接触角(WCA)、X射线光电子能谱(XPS)及傅里叶变换红外光谱(FT-IR)分别分析IOL表面亲水性和表面化学元素组成变化；原子力显微镜(AFM)以考察改性后IOL表面形貌的改变。结果：静态水接触角检测显示经紫外光辐照接枝处理后的IOL前表面亲水性明显增强,静态接触角明显下降(p0.01),接触角变化程度与接枝时间有良好相关性。红外光谱、XPS分析进一步证实了紫外光辐照接枝处理后IOL表面的极性基团的引入以及MPC的成功接枝。AFM观察改性后IOL表面未出现明显损伤, 粗糙度与处理前相比无明显差异. 结论：紫外光辐照接枝技术能够成功在疏水性聚丙烯酸酯IOL前表面接枝亲水性单体MPC,这种方法制造的新型人工晶体具有前表面亲水后表面疏水的特性,具有同时防止术眼前节炎症和后囊膜浑浊等术后并发症的潜力。该表面改性技术操作流程简单、经济环保,可连续性作业,具有良好的市场化前景。
[Abstract]:Objective: to realize the hydrophilic modification of the front surface of hydrophobic intraocular lens (IOL) and retain the hydrophobic properties of the surface of IOL by using the advanced ultraviolet irradiation technique to graft hydrophilic monomer, and to explore a simple method to improve the biocompatibility of IOL. Economic, efficient methods to facilitate further industrial production. Methods: hydrophobic acrylate intraocular lens was pretreated by chemical and physical methods in order to achieve active initiation. The front surface of hydrophobic polyacrylate IOL was modified by UV irradiation grafting of hydrophilic monomer 2-methacryloxy ethyl phosphatidylcholine (MPC). The surface hydrophilicity and chemical composition of IOL were analyzed by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR), respectively, and the surface morphology of the modified IOL was investigated by atomic force microscopy (AFM). Results: the static water contact angle test showed that the surface hydrophilicity of IOL grafted by UV irradiation was obviously enhanced, and the static contact angle was obviously decreased (p 0.01). The change of contact angle had good correlation with grafting time. FTIR XPS analysis further confirmed the introduction of polar groups on the surface of IOL after UV irradiation grafting and the successful grafting of MPC. AFM observed that the surface of the modified IOL was not obviously damaged and rough. There was no significant difference in degree between before treatment and before treatment. Conclusion: the UV irradiation grafting technique can successfully graft hydrophobic polyacrylate IOL onto the hydrophilic monomer MPC.The new intraocular crystal prepared by this method has the characteristics of hydrophilicity and hydrophobicity. It has the potential to prevent postoperative complications such as anterior segment inflammation and posterior capsule opacification. The surface modification technology has the advantages of simple operation flow, economic and environmental protection, continuous operation and good market prospect.
【学位授予单位】：浙江大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R779.66

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