腺样体肥大并发分泌性中耳炎儿童的淋巴细胞亚群分布

发布时间：2018-06-17 22:29

  本文选题：腺样体肥大 + 分泌性中耳炎　； 参考：《实用医学杂志》2017年14期

【摘要】：目的检测腺样体肥大并发分泌性中耳炎患儿的腺样体中淋巴细胞亚群分布情况,并探讨其意义。方法腺样体肥大并发分泌性中耳炎患儿45例(研究组),腺样体肥大但无中耳炎的患儿34例(对照组),手术切除腺样体,用相应的荧光抗体标记细胞,用流式细胞仪测定两组儿童腺样体中的淋巴细胞水平。结果在HA/OME组中,淋巴细胞亚群CD3~+CD4~+T细胞(8.97%)、CD3~+CD8~+T细胞(4.27%)、CD3~+CD8~+Fas~+T细胞(1.24%)、CD19~+Fas~+B细胞(3.23%)所占比例均显著高于HA组CD3~+CD4~+T细胞(6.37%)、CD3~+CD8~+T细胞(3.61%)、CD3~+CD8~+Fas~+T细胞(0.80%)、CD19~+Fas~+B细胞(2.68%)所占比例。在HA/OME组中CD3~+CD4~+CD69~+T细胞(1.29%)、CD3~+CD4~+HLA-DR~+T细胞(0.61%)、CD3~+CD8~+CD69~+T细胞(1.39%)、CD3~+CD8~+HLA-DR~+T细胞(0.09%)、CD19~+CD69~+B细胞(2.07%)、CD19~+CD62L~+B细胞(0.21%)所占比例均显著低于HA组的CD3~+CD4~+CD69~+T细胞(1.94%)、CD3~+CD4~+HLA-DR~+T细胞(0.84%)、CD3~+CD8~+CD69~+T细胞(2.08%)、CD3~+CD8~+HLA-DR~+T细胞(0.18%)、CD19~+CD69~+B细胞(3.98%)、CD19~+CD62L~+B细胞(0.46%)占比。结论肥大性腺样体并发分泌性中耳炎患者局部淋巴细胞比例改变,免疫功能失调,这可能与中耳炎的发生有一定关系。
[Abstract]:Objective to detect the distribution of lymphocyte subsets in adenoids of children with adenoid hypertrophy and secretory otitis media, and to explore its significance. Methods 45 cases of adenoid hypertrophy complicated with secretory otitis media (study group) and 34 cases of adenoid hypertrophy without otitis media (control group) were operated on to remove adenoids and labeled cells with corresponding fluorescent antibodies. The lymphocyte level in adenoid was measured by flow cytometry. Results in the HA / OME group, the percentage of CD3 ~ CD4 ~ T cells in the lymphocyte subgroup was significantly higher than that in the HA group. The percentage of CD3 ~ CD8 ~ T cells was 4.27 and the percentage of CD19 ~ FasB cells was 1.240.The percentage of CD3- CD4- T cells in HA group was higher than that of the HA group, and the percentage of CD3- CD8Fas-T cells was 0.80% (CD19 ~ FasB cells 2.68). 鍦℉A/OME缁勪腑CD3~ CD4~ CD69~ T缁嗚優(1.29%),CD3~ CD4~ HLA-DR~ T缁嗚優(0.61%),CD3~ CD8~ CD69~ T缁嗚優(1.39%),CD3~ CD8~ HLA-DR~ T缁嗚優(0.09%),CD19~ CD69~ B缁嗚優(2.07%),CD19~ CD62L~ B缁嗚優(0.21%)鎵，

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