血管活性肠肽对大鼠角膜碱烧伤后IL-1β的降低作用

发布时间：2018-06-17 22:42

本文选题：血管活性肠肽 + 角膜　；参考：《中国免疫学杂志》2017年11期

【摘要】：目的:探讨血管活性肠肽对大鼠角膜碱烧伤后不同时期IL-1β的降低作用。方法:将27只健康成年雌性Wistar大鼠,随机分为A组(正常空白对照组)、B组(碱烧伤生理盐水对照组)、C组(碱烧伤血管活性肠肽治疗组)。A组不处理,B、C组均制作右眼中度角膜碱烧伤模型。碱烧伤后B、C组分别用0.9%的生理盐水、0.25 g/L血管活性肠肽滴眼液滴眼,每日4次。碱烧伤后每天用显微镜观察眼前节情况,在碱烧伤后3、7、14 d通过亚甲蓝染色测量角膜着色面积,计算角膜上皮愈合率。3、7、21 d时分别取下碱烧伤侧角膜组织分为两等份,一份HE染色观察角膜组织结构,另一份进行角膜免疫组织化学法测定IL-1β的表达。结果:7 d、14 d角膜上皮愈合率C组大于B组(P0.05),差异有统计学意义。免疫组织化学法检测IL-1β平均光密度值结果显示:3 d时平均光密度值B组、C组分别为(1.62±0.69)×10~(-2)、(0.98±0.45)×10~(-2);7 d时B组、C组分别为(1.42±0.62)×10~(-2)、(0.71±0.22)×10~(-2);21 d时B组、C组分别为(0.37±0.24)×10~(-2)、(0.13±0.16)×10~(-2);各时间点大鼠角膜IL-1β平均光密度值C组水平均低于B组(P0.05),差异具有统计学意义。结论:血管活性肠肽能降低角膜碱烧伤后IL-1β的表达,下调免疫应激水平,减轻炎症反应,促进碱烧伤后角膜的恢复。
[Abstract]:Aim: to investigate the effect of vasoactive intestinal peptide (VAP) on IL-1 尾 in different stages of corneal alkali burn in rats. Methods: 27 healthy adult female Wistar rats were used. Group A was randomly divided into group A (normal blank control group) and group B (normal saline control group, group C) (alkali burn group treated with vasoactive intestinal peptide). Rats in group B were treated with 0.9% normal saline 0.25 g / L vasoactive intestinal peptide eye drops 4 times a day. After alkali burn, the anterior segment was observed by microscope every day. The corneal staining area was measured by methylene blue staining on the 3rd day after alkali burn, and the corneal epithelium healing rate was calculated. The corneal tissue of the alkali burn side was divided into two equal parts at 21 days after alkali burn. One was stained with HE to observe the structure of cornea, the other was stained with immunohistochemistry to detect the expression of IL-1 尾. Results the healing rate of corneal epithelium in group C was higher than that in group B (P 0.05) at 7 d to 14 d, and the difference was statistically significant. The average optical density of IL-1 尾 in group B was 1.62 卤0.69 脳 10 ~ (-1) 卤0.69) 0.98 卤0.45) 脳 10 ~ (10) ~ (-2) ~ (-1) ~ 0.45 脳 10 ~ (10) ~ (-2) ~ (2) at 7 days after 7 days, the mean optical density of group B was 1.42 卤0.62 脳 10 ~ (-2) 脳 10 ~ (-2) and 0.71 卤0.22) 脳 10 ~ (-10) ~ (-2) ~ (-1) on day 21, respectively, and that of group C was 0.37 卤0.24) 脳 10 ~ (-2) ~ (-1) ~ (0.16) 脳 10 ~ (10) ~ (-2) and the mean light density of group B was 0.37 卤0.24 脳 10 ~ (-2). The level of degree in group C was lower than that in group B (P 0.05), and the difference was statistically significant. Conclusion: vasoactive intestinal peptide can decrease the expression of IL-1 尾 after alkali burn of cornea, decrease the level of immune stress, alleviate the inflammatory reaction, and promote the recovery of cornea after alkali burn.
【作者单位】：锦州医科大学济南军区总医院研究生培养基地眼科;
【基金】：2014年度军区后勤科研计划重点课题(CJN14L017)
【分类号】：R779.13

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