多聚免疫球蛋白受体在鼻息肉组织中的表达及意义

发布时间：2018-06-20 04:46

本文选题：鼻息肉 + 多聚免疫球蛋白受体　；参考：《临床耳鼻咽喉头颈外科杂志》2017年01期

【摘要】：目的:研究多聚免疫球蛋白受体(pIgR)在鼻息肉组织中的表达水平,并初步探讨pIgR在鼻息肉发病机制中的作用。方法:选取36例鼻息肉患者的鼻息肉组织标本为实验组,选取同期行鼻内镜治疗的12例鼻中隔偏曲患者为对照组,取下鼻甲组织标本。术后行苏木精-伊红染色评估鼻息肉组织中嗜酸粒细胞(Eos)的浸润情况,并依据Eos的浸润程度分为嗜酸粒细胞性鼻息肉组(Eos CRSwNP组,21例)和非嗜酸粒细胞性鼻息肉组(non-Eos CRSwNP组,15例)。采用免疫组织化学法检测组织中pIgR和IgA的表达情况,实时定量聚合酶链反应(RT-PCR)法检测组织中pIgR、IgA、人类维甲酸孤儿受体(RORc)及叉头转录因子(Foxp3)mRNA的表达水平,统计分析鼻息肉组织中pIgR的表达及与Eos数量、辅助性T细胞(Th17细胞)及调节性T细胞(Treg细胞)转录因子的相关性。结果:pIgR在实验组中的表达显著低于对照组,差异有统计学意义(P0.05);Eos CRSwNP组中pIgR的表达低于non-Eos CRSwNP组,差异有统计学意义(P0.05)。IgA在实验组中的表达显著高于对照组,差异有统计学意义(P0.05)。RT-PCR分析结果显示:实验组中pIgR mRNA和Foxp3mRNA的表达低于对照组(P0.05),RORc mRNA和IgA mRNA的表达高于对照组(P0.05)。实验组中pIgR mRNA与RORc mRNA的表达呈负相关(P0.05,r=-0.79),与Foxp3mRNA的表达无相关性(P0.05,r=0.36)。结论:pIgR在鼻息肉组织中的表达下调,可能在鼻息肉的发生和发展中起着重要作用。
[Abstract]:Aim: to investigate the expression of polyimmunoglobulin receptor (PIgR) in nasal polyps and the role of pIgR in the pathogenesis of nasal polyps. Methods: the specimens of nasal polyps from 36 patients with nasal polyps were selected as experimental group and 12 patients with nasal septum deviation treated with nasal endoscopy as control group. The infiltration of eosinophils in nasal polyps was evaluated by hematoxylin and eosin staining after operation. According to the degree of Eos infiltration, the patients were divided into two groups: Eos CRSwNP group (n = 21) and non-eosinophil nasal polyp group (n = 15). The expression of pIgR and IgA in tissues was detected by immunohistochemical method, and the expression levels of pIgR IgA, human ORC receptor RORc) and forked transcription factor Foxp3 mRNA in tissues were detected by real-time quantitative polymerase chain reaction (RT-PCR). The expression of pIgR in nasal polyps and its correlation with the number of Eos, helper T cell Th17 cells and regulatory T cell / Treg cell) transcription factors were analyzed statistically. Results the expression of pIgR in the experimental group was significantly lower than that in the control group, and the expression of pIgR in the Eos CRSwNP group was significantly lower than that in the non-Eos CRSwNP group, and the difference was statistically significant. The expression of pIgR in the experimental group was significantly higher than that in the control group, and the expression of pIgR in the Eos CRSwNP group was significantly higher than that in the non-Eos CRSwNP group. The results of RT-PCR analysis showed that the expression of pIgR mRNA and Foxp3 mRNA in the experimental group was lower than that in the control group, and the expression of RORc mRNA and IgA mRNA in the control group was higher than that in the control group. In the experimental group, the expression of pIgR mRNA was negatively correlated with the expression of RORc mRNA, but not correlated with the expression of Foxp3 mRNA. Conclusion the down-regulation of the expression of the protein in nasal polyps may play an important role in the occurrence and development of nasal polyps.
【作者单位】：武汉大学人民医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金资助项目(No:81371070)
【分类号】：R765.25

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