Hsa-miR-193b对喉鳞状细胞癌Hep-2细胞生物学功能的影响

发布时间：2018-06-21 21:51

本文选题：喉肿瘤 + 鳞状细胞癌　；参考：《临床耳鼻咽喉头颈外科杂志》2017年07期

【摘要】：目的:研究hsa-miR-193b对喉鳞状细胞癌Hep-2细胞增殖、凋亡等生物学功能的作用。方法:实验分组为Hep-2组、NC组(Hep-2细胞+miR-193binhibitor NC)、miR-193b抑制组(Hep-2细胞+miR-193binhibitor)。采用qRT-PCR检测miR-193b的表达,MTT法检测细胞增殖,流式细胞仪检测细胞周期以及凋亡,Transwell检测细胞迁移能力。采用生物信息学技术进行靶基因预测,Western blot检测转染miR-193b后Hep-2细胞SMAD3的表达情况。结果:转染miR-193binhibitor后,miR-193b抑制组细胞miR-193b的表达显著降低,与Hep-2组和NC组比较差异有统计学意义(P0.01),后2组miR-193b表达量差异无统计学意义(P0.05)。和NC组比较,miR-193b抑制组的Hep-2细胞增殖能力在24、48和72h显著降低(P0.05或P0.01),G1/S期的细胞比例增多(P0.01),细胞凋亡明显增多(P0.01),细胞迁移能力显著降低(P0.01)。生物学信息分析预测miR-193b靶基因为SMAD3,Hep-2细胞转染miR-193b后与NC组相比,SMAD3蛋白表达显著下调(P0.05)。结论:miR-193b抑制剂能够抑制Hep-2细胞的增殖、迁移,促进细胞凋亡,提示miR-193b对喉鳞状细胞癌起到"癌基因"的作用,有望成为临床治疗的新靶点。
[Abstract]:Aim: to study the effects of hsa-miR-19b on proliferation and apoptosis of laryngeal squamous cell carcinoma (LSCC) Hep-2 cells. Methods: the experimental group was divided into two groups: Hep-2 cell miR-19binor NC (Hep-2 cell miR-19bininhibitor NC) and Hep-2 cell miR-193b inhibitor (Hep-2 cell miR-19bininhibitor). The expression of miR-19b was detected by qRT-PCR, cell proliferation was detected by MTT assay, cell cycle was detected by flow cytometry, and cell migration was detected by apoptosis Transwell. The expression of SMAD3 in Hep-2 cells after miR-193b transfection was detected by using bioinformatics technique and Western blot was used to detect the expression of SMAD3 in Hep-2 cells. Results: after transfection of miR-19bininhibitor, the expression of miR-193b was significantly decreased in miR-193b group, which was significantly lower than that in Hep-2 group and NC group (P 0.01), but there was no significant difference in the expression of miR-193b between the latter two groups (P 0.05). Compared with NC group, the proliferation ability of Hep-2 cells in the control group decreased significantly (P0.05 or P0.01) at 24: 48 and 72 h (P0.05 or P0.01), the percentage of cells increased (P0.01), apoptosis increased (P0.01), and the migration ability of Hep-2 cells decreased significantly (P0.01). Biological information analysis predicted that the expression of SMAD3 protein in miR-193b cells was significantly decreased after transfection of miR-193b cells compared with NC group (P0.05). Conclusion the cell proliferation, migration and apoptosis of Hep-2 cells can be inhibited by the inhibitor of miR-193b, which suggests that miR-193b may play an oncogene role in laryngeal squamous cell carcinoma and may be a new target for clinical therapy.
【作者单位】：山西医科大学第一医院耳鼻咽喉头颈外科耳鼻咽喉头颈肿瘤山西省重点实验室山西医科大学耳鼻咽喉研究所;
【基金】：山西省研究生教育创新项目(No:2016BY076) 山西医科大学第一医院青年基金(No:YQ1202)
【分类号】：R739.65

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