FDI-6对喉癌细胞恶性生物学行为的影响及其机制

发布时间：2018-06-23 23:23

本文选题：叉头结构域抑制物(FDI-6) + 叉头盒M1(FoxM1)　；参考：《重庆医科大学》2017年硕士论文

【摘要】：目的:探讨新型小分子抑制剂叉头结构域抑制物FDI-6(Forkhead Domain Inhibitory-6,FDI-6)对喉癌Hep-2细胞增殖、凋亡、侵袭和迁移的影响及其可能机制。方法:采用MTT法检测不同浓度(5~80)μmol/L FDI-6处理12 h、24 h后,Hep-2细胞的增殖情况。采用流式细胞术(flow cytometry,FCM)检测(10、20)μmol/L FDI-6处理Hep-2细胞24h后细胞凋亡情况、Transwell小室法检测细胞侵袭及迁移能力的变化。实时荧光定量PCR(Quantitative Real-time PCR,qRT-PCR)检测叉头盒M1(FoxM1)的mRNA水平、蛋白质免疫印记法检测FoxM1、Bcl-2、Bax的蛋白表达水平。结果:FDI-6可抑制喉癌Hep-2细胞的增殖,其抑制作用呈浓度和时间依赖性(P值均0.05)。与DMSO对照组相比,FDI-6处理组细胞凋亡率明显上升,细胞的侵袭、迁移能力明显受抑,且浓度越大,抑制作用越明显(P0.05)。FDI-6处理喉癌Hep-2细胞24 h后FoxM1mRNA和蛋白水平无明显改变(p0.05)。Bcl-2蛋白表达下降,Bax蛋白表达升高(p0.05),而在细胞核中FoxM1的蛋白水平随FDI-6浓度升高而降低(p0.05)。结论:FDI-6可抑制喉癌细胞Hep-2的增殖、侵袭、迁移,并诱导细胞凋亡,可能与下调细胞核中FoxM1及诱导肿瘤细胞凋亡相关。
[Abstract]:Aim: to investigate the effects of a novel small molecular inhibitor, forkhead domain inhibitor FDI-6 (FDI-6), on the proliferation, apoptosis, invasion and migration of laryngeal cancer cell line Hep-2 and its possible mechanism. Methods: MTT assay was used to detect the proliferation of Hep-2 cells treated with different concentrations of (5o 80) 渭 mol / L FDI-6 for 12 h or 24 h. The apoptosis of Hep-2 cells was detected by flow cytometry (10 ~ 20 渭 mol / L FDI-6) for 24 hours. The mRNA level of forkhead box M1 (FoxM1) was detected by quantitative real-time PCR and the protein expression level of FoxM1Bcl-2mBax was detected by protein imprinting. Results the proliferation of Hep-2 cells was inhibited by 1% FDI-6 in a concentration and time dependent manner (P < 0.05). Compared with the DMSO control group, the apoptosis rate of the treated group was significantly increased, the invasion and migration ability of the cells were inhibited obviously, and the higher the concentration was, the higher the cell apoptosis rate was. The inhibitory effect was more obvious (P0.05). FDI-6 had no obvious changes in FoxM1 mRNA and protein levels (p0.05) .Bcl-2 protein expression decreased (p0.05), but FoxM1 protein level decreased with the increase of FDI-6 concentration (p0.05). Conclusion the cell proliferation invasion migration and apoptosis of laryngeal carcinoma cell line Hep-2 can be inhibited by WFDI-6 which may be related to the down-regulation of FoxM1 in the nucleus and the induction of apoptosis in tumor cells.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.65

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