先天性上斜肌麻痹性斜视患儿眼外肌NGF、GAP-43表达变化及意义

发布时间：2018-06-28 03:54

本文选题：儿童 + 先天性麻痹性斜视　；参考：《山东医药》2017年14期

【摘要】：目的探讨神经生长因子(NGF)及生长相关蛋白(GAP-43)在儿童先天性上斜肌麻痹性斜视发病中的意义。方法选取28例先天性上斜肌麻痹性斜视患儿行斜视矫正手术中获取的病变上斜肌(斜视上斜肌组)15例、下斜肌(斜视下斜肌组)13例,另选取30例因眼外伤行眼球摘取手术患儿的正常上斜肌(正常上斜肌组)、正常下斜肌(正常下斜肌组)组织各15例。采用HE染色法观察各组上下斜肌组织病理改变。采用免疫组化染色法检测各组上下斜肌组织NGF及GAP-43蛋白表达。结果正常上斜肌组、斜视上斜肌组NGF蛋白阳性表达率为分别为80.0%、46.7%,正常下斜肌组、斜视下斜肌组NGF蛋白阳性表达率分别为73.3%、84.6%,斜视上斜肌组NGF蛋白阳性表达率低于正常上斜肌组,斜视下斜肌组NGF蛋白阳性表达率高于正常下斜肌组(P0.05)。正常上斜肌组、斜视上斜肌组GAP-43蛋白阳性表达率分别为73.3%、33.3%,正常下斜肌组、斜视下斜肌组GAP-43蛋白阳性表达率分别为60.0%、84.6%,斜视上斜肌组GAP-43蛋白阳性表达率低于正常上斜肌组,斜视下斜肌组GAP-43蛋白阳性表达率高于正常下斜肌组(P0.05)。结论 NGF及GAP-43在先天性上斜肌麻痹性斜视患儿上斜肌中表达降低、在下斜肌中表达增强,二者在先天性上斜肌麻痹性斜视发病中具有重要作用。
[Abstract]:Objective to investigate the significance of nerve growth factor (NGF) and growth associated protein (GAP-43) in congenital superior oblique paralysis in children. Methods Fifteen children with congenital superior oblique palsy strabismus and 13 patients with inferior oblique muscle were treated with strabismus correction surgery, including 15 patients with superior oblique muscle (superior oblique muscle group) and 13 patients with inferior oblique muscle group. 30 cases of normal superior oblique muscle (normal superior oblique muscle group) and 15 cases of normal inferior oblique muscle (normal inferior oblique muscle group) were selected. The histopathological changes of superior and inferior oblique muscles were observed by HE staining. The expression of NGF and GAP-43 in superior and inferior oblique muscle was detected by immunohistochemical staining. Results the positive expression rates of NGF protein in the normal superior oblique muscle group and the strabismus superior oblique muscle group were 80.0 and 46.7, respectively. The NGF protein positive expression rates in the normal inferior oblique muscle group and the strabismus inferior oblique muscle group were 73.3% and 84.6%, respectively. The NGF protein positive expression rate in the strabismus superior oblique muscle group was lower than that in the normal superior oblique muscle group. The positive expression rate of NGF protein in strabismus inferior oblique muscle group was higher than that in normal inferior oblique muscle group (P0.05). The positive expression rates of GAP-43 protein in normal superior oblique muscle group, superior oblique muscle group and normal inferior oblique muscle group were 73.3 and 33.33.The positive expression rates of GAP-43 protein in normal inferior oblique muscle group and strabismus inferior oblique muscle group were 60.084.6g, respectively, and the positive expression rate of GAP-43 protein was lower in strabismus superior oblique muscle group than that in normal superior oblique muscle group, the positive expression rate of GAP-43 protein in superior oblique muscle group was lower than that in normal superior oblique muscle group. The positive expression rate of GAP-43 protein in strabismus inferior oblique muscle group was higher than that in normal inferior oblique muscle group (P0.05). Conclusion the expression of NGF and GAP-43 in the superior oblique muscle of children with congenital superior oblique palsy strabismus is decreased, and the expression of GAP-43 is increased in the inferior oblique muscle. They play an important role in the pathogenesis of congenital superior oblique paralysis strabismus.
【作者单位】：河北省眼科医院;
【分类号】：R777.41

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