抗抑郁药地昔帕明对变应性鼻炎小鼠的缓解作用

发布时间：2018-06-28 00:07

本文选题：地昔帕明 + 变应性鼻炎　；参考：《华中科技大学》2012年硕士论文

【摘要】：背景：变应性鼻炎是由IgE介导的超敏反应。它是由于鼻粘膜对某些刺激因子过度敏感而产生超出生理范围的鼻粘膜炎症反应。地昔帕明属于三环类抗抑郁药，同时有报道指出它对酸性鞘磷脂酶具有抑制作用。酸性鞘磷脂酶在炎症反应过程中发挥着必不可少的作用。地昔帕明对变应性鼻炎小鼠的作用效果尚未有相关的报道。因此，本实验的研究目的为，抗抑郁药地昔帕明对变应性鼻炎小鼠的鼻部症状是否疗效。方法：雄性BALB/C小鼠用卵清蛋白和氢氧化铝混合液腹腔注射致敏，激发阶段用卵清蛋白滴鼻。变应性鼻炎模型造模成功后，每次卵清蛋白滴鼻后，地昔帕明灌胃。观察变应性鼻炎小鼠的行为学，包括喷嚏和抓鼻次数，来判患病小鼠的严重程度。通过ELISA的方法检测血清卵清蛋白特异性IgE及鼻腔灌洗液中细胞因子IL-4和INF-γ的水平。并且应用流式细胞仪来检测小鼠脾细胞中调节性T细胞和Th17细胞来探讨相关的作用机制。结果：反复用地昔帕明灌胃可以减轻受激发小鼠过敏症状（喷嚏及抓鼻次数）的发生，并且与地昔帕明的计量呈相关性。它还可以减少血清中卵清蛋白特异性IgE的水平和鼻腔灌洗液中IL-4的水平。尽管如此，地昔帕明对鼻腔灌洗液中INF-γ的水平没有明显的作用。此外，地昔帕明还可以通过对Treg细胞和Th17细胞进行调节来削弱变应性鼻炎的气道炎症反应。结论：地昔帕明可以通过抑制产生血清卵清蛋白特异性IgE及IL-4，，并且调节脾细胞中CD4+CD25+Foxp3+T细胞和CD4+Th17细胞的比例来发挥其抗过敏的功效。在变应性鼻炎的发病机制中，除已知的Th1/Th2平衡外，还存在Treg/Th17平衡，这为我们研究变应性鼻炎免疫学机制提供了一条新的理论依据，为以后变应性鼻炎的治疗提供了新思路。
[Abstract]:Background: allergic rhinitis is a hypersensitive response mediated by IgE. It is due to nasal mucosal hypersensitivity to certain irritants and the production of physiological range of nasal inflammation. Despermine is a tricyclic antidepressant and has been reported to have inhibitory effects on acid sphingolipase. Acid sphingolipase plays an essential role in inflammatory reaction. There is no report on the effect of desparmine on allergic rhinitis in mice. Therefore, the aim of this study was to determine the efficacy of the antidepressant Dexiparam on nasal symptoms in allergic rhinitis mice. Methods: male BALB / C mice were sensitized by intraperitoneal injection of ovalbumin and aluminum hydroxide. After the model of allergic rhinitis was established successfully, after ovalbumin drops, desicamine was administered to the stomach. Observe the behavior of allergic rhinitis mice, including sneezing and nasal scratching times, to determine the severity of the disease in mice. The levels of serum ovalbumin specific IgE and cytokines IL-4 and INF- 纬 in nasal lavage fluid were detected by Elisa. Flow cytometry was used to detect regulatory T cells and Th17 cells in mouse spleen cells. Results: repeated oral administration of dixipramine reduced the incidence of allergic symptoms (sneezing and nasal scratching) in stimulated mice, and was correlated with the metrology of desipramine. It also reduced the serum level of ovalbumin specific IgE and the level of IL-4 in nasal lavage fluid. Nevertheless, desipramine had no significant effect on the level of INF- 纬 in nasal lavage fluid. In addition, despermine can weaken the airway inflammation of allergic rhinitis by regulating Treg cells and Th17 cells. Conclusion: Despermine can play its antiallergic effect by inhibiting the production of serum ovalbumin specific IgE and IL-4, and regulating the ratio of CD4 CD25 Foxp3 T cells to CD4 Th17 cells in spleen cells. In the pathogenesis of allergic rhinitis, in addition to the known Th1 / Th2 balance, there is Treg-Th17 balance, which provides a new theoretical basis for us to study the immune mechanism of allergic rhinitis, and provides a new idea for the treatment of allergic rhinitis.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R765.21

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