硫酸锌对紫外线诱导的大鼠晶状体氧化损伤的保护作用

发布时间：2018-07-18 11:11

【摘要】：目的 探讨硫酸锌（ZnSO_4）对紫外线诱导的大鼠晶状体氧化损伤的保护作用。 方法 培养离体大鼠晶状体，，将其随机分为三组：（1）阴性对照组（正常组）；（2）阳性对照组（紫外线照射组）；（3）实验组(紫外线照射+ZnSO_4组)。观察晶状体的混浊程度，取各组晶状体标本进行苏木素/伊红染色（HE）染色，光镜下观察组织形态学变化，检测晶状体组织中谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）及晶状体中热休克蛋白70（HSP70）表达的变化。 结果 阴性对照组各时段的晶状体基本保持透明；阳性对照组晶状体随着培养时间的延长逐渐混浊；实验组晶状体随着培养时间的延长逐渐混浊，但与同时段阳性对照组相比，混浊程度减轻。光学显微镜下见阴性对照组晶状体组织结构基本正常；阳性对照组与实验组晶状体组织结构有不同程度的损伤；实验组晶状体损伤程度较同时段阳性对照组减轻。阳性对照组晶状体中GSH-Px和SOD活性低于同时段阴性对照组，差异有统计学意义（P0.05）；实验组晶状体中GSH-Px和SOD活性高于同时段阳性对照组，差异有统计学意义（P0.05）。阳性对照组晶状体中HSP70的表达与同时段阴性对照组相比，表达增加，差异有统计学意义（P0.05）；实验组晶状体中HSP70的表达与同时段阳性对照组相比，表达增加，差异有统计学意义（P0.05）。 结论 ZnSO_4可以诱导离体大鼠晶状体中HSP70的表达，可以提高氧化损伤晶状体的抗氧化能力，在维持晶状体透明性方面起到了一定的作用。
[Abstract]:Objective to investigate the protective effect of zinc sulfate (ZnSO _ 4) on ultraviolet induced oxidative injury of rat lens. Methods isolated rat lens were cultured and randomly divided into three groups: (1) negative control group (normal group,); (_ 2), positive control group (ultraviolet radiation group,); (_ 3), experimental group (ultraviolet radiation); (_ 3 group). The degree of opacity of lens was observed. Hematoxylin / eosin (HE) staining and histomorphology were observed under light microscope. The changes of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and heat shock protein 70 (HSP70) in lens were detected. Results the lens of the negative control group was basically transparent at every time, the lens of the positive control group was gradually opacified with the extension of culture time, and the lens of the experimental group was gradually opacified with the extension of the culture time. But compared with the positive control group at the same time, the degree of turbidity decreased. Under the optical microscope, the structure of the lens in the negative control group was basically normal, that in the positive control group and the experimental group was different degrees, and the degree of lens injury in the experimental group was less than that in the positive control group in the same period. The activities of GSH-Px and SOD in lens of positive control group were lower than those of negative control group in the same period (P0.05); the activities of GSH-Px and SOD in lens of experimental group were higher than that of positive control group in the same period (P0.05). The expression of HSP70 in the lens of the positive control group was higher than that of the negative control group in the same period (P0.05), while the expression of HSP70 in the lens of the experimental group was higher than that of the positive control group in the same period (P0.05). Conclusion ZnSOS _ 4 can induce the expression of HSP70 in isolated rat lens, increase the anti-oxidation ability of the injured lens, and play a certain role in maintaining the transparency of the lens.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R77

【参考文献】

相关期刊论文 前10条

1 李义杰;张保国;;热休克蛋白的生物学功能及其对细胞的辐射防护作用[J];辐射防护通讯;2007年06期

2 任宝波,王玉艳,王纯净,李士泽,杨焕民;HSP70家族的分类及基因结构与功能[J];动物医学进展;2005年01期

3 仝佳;王胜军;肖寿华;;热休克蛋白及其在眼部疾病中作用的研究进展[J];国际眼科杂志;2006年01期

4 周星利;亢泽峰;李凌;马雪英;张蓉;;热休克蛋白与白内障发生的研究进展[J];国际眼科杂志;2008年04期

5 刘瑜;鲁建华;张文芳;李姗姗;曲来强;;谷氨酰胺体外对大鼠晶状体上皮细胞HSP70和NF-κB表达的影响[J];国际眼科杂志;2010年09期

6 饶惠英,姚克;热休克蛋白在眼晶状体的表达与热耐受[J];国外医学(眼科学分册);2004年03期

7 宫智勇;牛丕业;谭皓;王峰;邹云锋;杨瑾;段燕英;袁晶;邬堂春;;槲皮素抑制HSP70表达的体外研究[J];工业卫生与职业病;2007年02期

8 李莹;王玉清;曹丽辉;冯澜;;热休克蛋白70在老年性白内障晶状体上皮细胞中的表达及意义[J];黑龙江医药科学;2006年04期

9 杨震国;;热休克蛋白70的生物学功能及应用的研究进展[J];中国饲料;2010年23期

10 周云;微量元素与眼病[J];微量元素与健康研究;2004年05期

本文编号：2131722