兔眼后Tenon囊下和球周注射曲安奈德后眼内药物浓度的研究

发布时间：2018-07-21 16:13

【摘要】：目的分别通过从兔眼后Tenon氏囊下和球周注射曲安奈德之后,然后在五个关键的已知时间点,取兔眼玻璃体和视网膜组织,并分析TA在这两种组织内的药物浓度,为TA在临床中应用,提供一定的实验依据供参考。方法在实验动物中心选购健康的成年灰色家兔30只,无需要求性别,要求一般体重3kg左右。随机分成两大组,即：后Tenon氏囊下组和球周组,两大组家兔的右眼均为实验眼,分别给予TA40mg/0.3ml后Tenon氏囊下注射和TA40mg/0.3ml球周注射。两大组家兔的左眼无注射操作。注药前及注药后观察眼表和眼内各组织有无改变,记录眼压。并于给药后1天,3天,7天,14天和28天五个关键的时间点,每组随机处死3只家兔,取兔眼的玻璃体、视网膜组织,采用高效液相色谱仪测TA的浓度,进行统计学分析。结果兔眼视网膜线性回归方程为Y=0.604X+0.0691,(R2=0.9997,P0.05)；玻璃体线性回归方程为Y=0.6258X+0.0372,(R2=0.9998,P0.05)；经后Tenon氏囊下给药后,第1天：视网膜中TA浓度为(1.555±0.654)μg·g-1、玻璃体腔中TA浓度为(0.729±0.375)μg·ML-1,第3天：视网膜中TA浓度为(11.850±4.427)μg·g-1、玻璃体腔中TA浓度为(1.62±0.539)μg·mL-1,第3天浓度最高,以后逐渐下降；而球周给药后,第1天视网膜中TA浓度为(0.157±0.058)μg.g-1、玻璃体腔中TA浓度为(0.050±0.027)μg·mL-1,第3天时药物浓度均达到最大,视网膜中TA浓度为为(1.040±0.292)μg·g-1、玻璃体腔中TA浓度为(0.143±0.070)μg-mL-1。整个观察期,所有家兔均未发生因注药操作及药物TA所致的并发症。结论1.分别经后Tenon氏囊下和球周注射40rrgTA后,在五个关键的时间点均能在兔眼视网膜组织及玻璃体腔内检测到一定浓度的TA。 2.参考文献,在整个观察期间,视网膜组织及玻璃体腔中的TA浓度一直在有效治疗浓度之上,并维持一定时间。 3.在本实验的研究条件下,经后Tenon氏囊下注射得到的兔眼内组织中曲安奈德的浓度比球周注射得到的浓度更高,且两者浓度差异具有统计学意义。
[Abstract]:Objective to obtain the vitreous and retinal tissue of rabbit eyes by injecting triamcinolone acetonide from the rabbit's posterior Tenon and the five key known time points, and to analyze the drug concentration of TA in these two tissues, and to provide some reference for the clinical application of TA in clinical practice.
Methods 30 healthy adult gray rabbits were selected at the experimental animal center without requiring sex and required about 3kg of the general body weight. They were randomly divided into two groups, namely, the posterior Tenon sac group and the week group, the right eyes of the two groups of rabbits were all experimental eyes, and the two groups were given TA40mg/0.3ml after Tenon's capsule injection and TA40mg/0.3ml. There was no injection in the left eye of the rabbit. Before and after injection, the ocular surface and the intraocular tissues were observed or not, and the intraocular pressure was recorded. At 1 days, 3 days, 7 days, 14 days and 28 days after administration, 3 rabbits were killed in each group. The vitreous and retinal tissue of the rabbit eyes were collected and the concentration of TA was measured by high performance liquid chromatograph. Study analysis.
Results the linear regression equation of the rabbit eye retina was Y=0.604X+0.0691, (R2=0.9997, P0.05); the linear regression equation of vitreous body was Y=0.6258X+0.0372, (R2=0.9998, P0.05); after the administration of the post Tenon capsule, the concentration of TA in the retina was (1.555 + 0.654) mu g. G-1, the TA concentration in the vitreous cavity was (0.729 + 0.375) micron G. Third days: the optic network The concentration of TA in the membrane is (11.850 + 4.427) mu g. G-1, the concentration of TA in the vitreous cavity is (1.62 + 0.539) mu g. ML-1, the concentration of the third days is the highest and then gradually descends, and the concentration of TA in the retina membrane is (0.157 + 0.058) mu g.g-1, and the concentration of TA is (0.050 + 0.027) mu mL-1, and the concentration of the drug reaches the maximum in the retina on the third day, and the concentration of the retina reaches the maximum. The concentration of TA was (1.040 + 0.292) mu g. G-1, and the concentration of TA in the vitreous cavity was (0.143 + 0.070) mu g-mL-1. in the whole observation period. All rabbits had no complications caused by drug injection and drug TA.
Conclusion 1. a certain concentration of TA. can be detected in the retinal tissue and the vitreous cavity of the rabbit eye at five critical time points after the 1. subsacs and the intravitre injection of 40rrgTA respectively.
2. according to the references, during the whole observation period, the concentration of TA in the retinal tissue and vitreous cavity has been above the effective therapeutic concentration and maintained for a certain time.
3. under the conditions of this experiment, the concentration of triamcinolone acetonide in rabbit's intraocular tissue was higher than that of the intravitre injection of the posterior Tenon's capsule, and the difference of the concentration was statistically significant.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774;R776

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