AQP1在声带息肉中表达的研究及意义
发布时间：2018-07-21 16:40
【摘要】：目的：通过检测AQP1在声带息肉组织中的表达情况,探讨其在声带息肉发生、发展过程中的作用,为临床防治提供理论依据。方法：采用免疫组织化学技术SP法及HE染色,检测AQP1在40例声带息肉和20例正常声带组织中的表达情况,应用Image-Proplus图像分析软件测定免疫组化图像,运用SPSS17.0统计软件对实验组和对照组各数据进行分析,做t检验及相关性检验,计算出P值(取P0.05为差异有显著性)。结果：AQP1在声带息肉组织中的上皮细胞、炎性细胞、血管内皮细胞、腺体细胞、成纤维细胞表达较强,在正常声带组织中的表达较弱。声带息肉组AQP1阳性单位均值(39.9633±8.7820)高于正常声带组(17.4222±8.5380),有统计学差异(p0.05)。男、女组间无显著差异(P0.05)。结论：①AQP1在声带息肉组织中的高表达,提示其与声带息肉的发生、发展可能有关系,有望为预防和治疗声带息肉提供依据。②声带息肉发展过程中,合成AQP1促进息肉血管的形成,息肉血管增生,息肉获得丰富的血供从而不断生长。③AQP1在浆液性腺细胞的高表达,可致大量的水样浆液分泌渗出,AQP1在上皮下毛细管及血窦内皮细胞的高表达,使血管通透性增强,血浆渗出,二者导致了组织间液增多。同时,AQP1在上皮基底细胞和纤毛细胞层无表达或少许表达,致使液体运输通路障碍,不利于液体转运,使分泌和渗出到组织间隙中的大量液体积聚。上述两种情形终至局部组织形成水肿,息肉形成。
[Abstract]:Objective: to investigate the role of AQP1 in the occurrence and development of vocal cord polyps by detecting the expression of AQP1 in vocal cord polyps, and to provide a theoretical basis for clinical prevention and treatment. Methods: the expression of AQP1 in 40 cases of vocal cord polyps and 20 cases of normal vocal cord tissues were detected by immunohistochemical technique SP method and HE staining. The immunohistochemical images were detected by Image-Proplus image analysis software. The statistical software SPSS 17.0 was used to analyze the data of the experimental group and the control group. T test and correlation test were done to calculate the P value (P 0.05 as the significant difference). Results the expression of VAQP1 in epithelial cells, inflammatory cells, vascular endothelial cells, glandular cells and fibroblasts in vocal cord polyps was stronger than that in normal vocal cord tissues. The mean value of AQP1 positive unit in vocal cord polyp group (39.9633 卤8.7820) was higher than that in normal vocal cord group (17.4222 卤8.5380), there was statistical difference (p0.05). There was no significant difference between male and female groups (P0.05). Conclusion the high expression of AQP1 in vocal cord polyps suggests that it may be related to the occurrence and development of vocal cord polyps, which may provide a basis for the prevention and treatment of vocal cord polyps. Synthesis of AQP1 promotes polyp angiogenesis, polyps with abundant blood supply and growth. 3 AQP1 is highly expressed in serous gonadal cells. High expression of AQP1 in capillary and sinusoidal endothelial cells, increased vascular permeability and plasma exudation, which led to the increase of interstitial fluid. At the same time, there was no or little expression of AQP1 in epithelial basal cells and ciliated cells, which resulted in the obstruction of liquid transport pathway and the accumulation of a large amount of liquid secreted and exudated into the interstitial tissue. Both cases end in edema and polyp formation in local tissues.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R767.4
本文编号：2136145
[Abstract]:Objective: to investigate the role of AQP1 in the occurrence and development of vocal cord polyps by detecting the expression of AQP1 in vocal cord polyps, and to provide a theoretical basis for clinical prevention and treatment. Methods: the expression of AQP1 in 40 cases of vocal cord polyps and 20 cases of normal vocal cord tissues were detected by immunohistochemical technique SP method and HE staining. The immunohistochemical images were detected by Image-Proplus image analysis software. The statistical software SPSS 17.0 was used to analyze the data of the experimental group and the control group. T test and correlation test were done to calculate the P value (P 0.05 as the significant difference). Results the expression of VAQP1 in epithelial cells, inflammatory cells, vascular endothelial cells, glandular cells and fibroblasts in vocal cord polyps was stronger than that in normal vocal cord tissues. The mean value of AQP1 positive unit in vocal cord polyp group (39.9633 卤8.7820) was higher than that in normal vocal cord group (17.4222 卤8.5380), there was statistical difference (p0.05). There was no significant difference between male and female groups (P0.05). Conclusion the high expression of AQP1 in vocal cord polyps suggests that it may be related to the occurrence and development of vocal cord polyps, which may provide a basis for the prevention and treatment of vocal cord polyps. Synthesis of AQP1 promotes polyp angiogenesis, polyps with abundant blood supply and growth. 3 AQP1 is highly expressed in serous gonadal cells. High expression of AQP1 in capillary and sinusoidal endothelial cells, increased vascular permeability and plasma exudation, which led to the increase of interstitial fluid. At the same time, there was no or little expression of AQP1 in epithelial basal cells and ciliated cells, which resulted in the obstruction of liquid transport pathway and the accumulation of a large amount of liquid secreted and exudated into the interstitial tissue. Both cases end in edema and polyp formation in local tissues.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R767.4
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