IL-16、IL-32在前葡萄膜炎患者血清中的表达及意义
发布时间：2018-07-23 15:54
【摘要】：目的:1观察本研究中葡萄膜炎患者的临床特点、治疗效果、视力预后、并发症以及诊治过程中存在的问题。2检测IL-16和IL-32在正常人及前葡萄膜炎患者血清中的表达情况,比较组间IL-16和IL-32表达水平有无明显差异,分别分析它们与疾病之间的相关性。3检测IL-16和IL-32在前葡萄膜炎活动期及稳定期患者血清中的表达情况,比较其表达水平有无差异,并检测IL-16和IL-32在前葡萄膜炎患者治疗前后血清中的表达情况,比较治疗前后有无明显差异,进一步分析它们与疾病的关系。4探讨IL-16和IL-32在前葡萄膜炎发病中的作用,寻找治疗前葡萄膜炎的新方法和新药物。方法:1收集自2015年1月至2017年1月于河北医科大学第一医院就诊的各类葡萄膜炎患者85例,其中男45例,女40例,平均发病年龄(41.25±16.83),发病年龄范围为7-70岁,详细记录他们的全身情况、眼病史以及临床诊疗情况。同期收集非葡萄膜炎患者40例作为正常对照组,其中男22例,女18例,平均年龄(42.33±12.68)。所有对照者排除葡萄膜炎病史,既往身体健康,近期未服用过抗生素、糖皮质激素、非甾体类激素和免疫抑制剂。2采集40例前葡萄膜炎实验组患者及40例正常对照组患者外周静脉血标本4-6ml,离心后提取血清,然后应用双抗体夹心法酶联免疫吸附试验(ELISA),来检测IL-16和IL-32在实验组及正常对照组血清中的表达情况,并对实验组及正常对照组行血沉(Erythrocyte Sedimentation Rate,ESR)、类风湿因子(Rheumatoid factor,RF)、C反应蛋白(C-Reactive protein,CRP)、抗链“O”等检查,分别将所得检测结果进行统计学分析。结果:1本研究85例葡萄膜炎患者中,21-45岁患者最多,发病年龄范围为7-70岁,男女比例为1.125:1。其中单眼发病患者67例,双眼同时受累的患者有18例。按解剖部位来看前葡萄膜炎(64例)最多,其次全葡萄膜炎13例,中间葡萄膜炎及后葡萄膜炎各4例,根据病程来分急性葡萄膜炎患者(61例)最多,慢性葡萄膜炎患者5例,复发性葡萄膜炎患者19例,其中伴有强直性脊柱炎的患者为7例,伴有vogt-小柳-原田(vkh)综合征的有6例(7.9%),伴有behcet病的为5例,合并fuchs综合征4例。本研究85例研究对象中有31例出现白内障、青光眼或眼压升高、虹膜粘连等并发症,大部分患者经过及时的积极的药物治疗后视力都有所提高。2前葡萄膜炎组患者血清中il-16和il-32的浓度分别为196.42(140.77,263.26)和265.67(214.51,342.82);正常对照组血清il-16和il-32的浓度分别为82.68(49.03,115.38)和86.19(51.57,154.28)。前葡萄膜炎组血清il-16浓度与正常对照组比较差异有统计学意义(p=0.003),前葡萄膜炎组血清il-32浓度与正常对照组比较差异也有统计学意义(p=0.008)。3前葡萄膜炎活动期患者血清il-16水平209.55(152.89,277.40)较稳定期水平97.19(57.92,123.17)升高,差异有统计学意义(p=0.000);活动期患者血清il-32水平278.98(222.12,346.80)较稳定期患者95.48(68.26,167.64)升高,差异有统计学意义(p=0.024)。4前葡萄膜炎患者治疗后血清il-16水平84.55(51.39,127.04)较治疗前水平196.42(140.77,263.26)显著降低,差异有统计学意义(p=0.004);并且前葡萄膜炎治疗后血清il-32水平88.98(52.17,165.43)较治疗前水平265.67(214.51,342.82)也显著降低,差异也有统计学意义(p=0.000)。5用spearman相关分别分析il-16和il-32与前葡萄膜炎疾病的相关性,结果显示il-16与疾病呈正相关(spearman相关系数r=0.671,p=0.000),il-32也与前葡萄膜炎呈正相关(spearman相关系数r=0.963,p=0.000)。il-16与il-32之间呈正相关(spearman相关系数r=0.383,p=0.009)。6前葡萄膜炎组患者esr、rf、crp、抗链“o”检测结果分别是24.50(15.50,36.50)、6.30(4.85,9.80)、64.10(11.30,113.10)和4.80(4.02,5.47);正常对照组esr、rf、crp、抗链“o”检测结果分别为15.60(6.90,27.70)、4.52(3.07,8.35)、47.50(8.10,95.80)和3.43(2.65,4.12)。前葡萄膜炎组患者ESR水平较正常对照组升高,差异有统计学意义(P=0.004)。两组患者之间RF、CRP、抗链“O”水平差异无统计学意义(RF:P=0.645;CRP:P=0.053;抗链“O”:P=0.620)。7用Spearman相关分别分析IL-16、IL-32与前葡萄膜炎组患者年龄、ESR、RF、CRP和抗链“O”的相关性,结果未发现血清IL-16、IL-32与年龄、ESR、RF、CRP和抗链“O”的相关性(P0.05)。结论:1本研究中发现葡萄膜炎在中青年人群中发病多见,前葡萄膜炎为临床常见类型,白内障和青光眼或眼压升高为常见并发症,大部分患者视力预后较好,少数出现视力损害。2前葡萄膜炎组患者血清IL-16、IL-32水平分别与正常对照组比较明显升高;IL-16、IL-32分别与疾病呈正相关,证实了IL-16、IL-32是前葡萄膜炎中重要的细胞因子,表明IL-16、IL-32参与了前葡萄膜炎的发病。3前葡萄膜炎活动期患者血清IL-16、IL-32水平较稳定期水平明显升高,证实了IL-16、IL-32与前葡萄膜炎的活动有关。4前葡萄膜炎患者治疗后血清IL-16、IL-32水平较治疗前水平明显降低,更进一步证实了IL-16、IL-32参与了前葡萄膜炎的发生发展。5血清中IL-16和IL-32之间呈正相关,认为其分别或协同参与了前葡萄膜炎的发生和演进。
[Abstract]:Objective: 1 to observe the clinical characteristics, therapeutic effect, visual prognosis, complications, and problems in the diagnosis and treatment of uveitis in this study,.2 detection of the expression of IL-16 and IL-32 in the serum of normal people and patients with anterior uveitis, and to compare the differences of IL-16 and IL-32 expression levels between groups and analyze them with the disease, respectively. The correlation.3 detected the expression of IL-16 and IL-32 in the sera of the active and stable patients with anterior uveitis, compared with the expression level, and detected the expression of IL-16 and IL-32 in the sera before and after the treatment of the patients with anterior uveitis. There was no significant difference before and after the treatment, and the relationship between them and the disease was further analyzed. .4 was used to explore the role of IL-16 and IL-32 in the pathogenesis of anterior uveitis, looking for new methods and new drugs for the treatment of pre uveitis. 1 a total of 85 cases of uveitis were collected from January 2015 to January 2017 in the first hospital of Hebei Medical University, including 45 males and 40 females, with an average age of onset (41.25 + 16.83), and the age range of onset. At the age of 7-70, the whole body condition, the history of ophthalmopathy and the clinical diagnosis and treatment were recorded in detail. 40 cases of non uveitis were collected in the same period as the normal control group, including 22 males and 18 females, with an average age of 42.33 + 12.68. All the controls excluded the history of uveitis, previous body health, no antibiotics, glucocorticoids, and non steroids. The body hormone and immunosuppressant.2 collected 40 cases of anterior uveitis and 40 cases of normal control group of peripheral venous blood samples 4-6ml, extracted serum after centrifugation, and then used the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the expression of IL-16 and IL-32 in the serum of the experimental group and the normal control group. The test group and the normal control group were Erythrocyte Sedimentation Rate (ESR), rheumatoid factor (Rheumatoid factor, RF), C reactive protein (C-Reactive protein, CRP) and anti chain O). The results were statistically analyzed. Results: 1 of the 85 cases of uveitis were the most 21-45 years old patients and age model. The peri was 7-70 years old, with a male and female ratio of 1.125:1. with 67 cases of monocular disease and 18 cases with double binocular involvement. According to the anatomical site, anterior uveitis (64 cases) was the most, followed by full uveitis in 13 cases, middle uveitis and 4 cases of posterior uveitis, according to the course of the disease (61 cases), the most, chronic uveitis There were 5 patients and 19 cases of recurrent uveitis, including 7 cases with ankylosing spondylitis, 6 cases (7.9%) with vogt- Xiao Liu Tian (VKH) syndrome, 5 cases with Behcet disease and 4 cases of Fuchs syndrome. In this study, 85 cases of the study included cataract, glaucoma or intraocular pressure, and synechia of the iris. The visual acuity of most patients after timely active drug treatment improved the concentration of IL-16 and IL-32 in the serum of.2 anterior uveitis group was 196.42 (140.77263.26) and 265.67 (214.51342.82), and the serum concentration of IL-16 and IL-32 in the normal control group was 82.68 (49.03115.38) and 86.19 (51.57154.28), respectively. The serum IL-16 concentration was significantly different from that of the normal control group (p=0.003). The serum IL-32 concentration in the anterior uveitis group was also significantly different from that of the normal control group (p=0.008) the serum IL-16 level 209.55 (152.89277.40) was higher than the stable level 97.19 (57.92123.17) in the patients with.3 anterior uveitis. Study significance (p=0.000); serum IL-32 level 278.98 (222.12346.80) of patients at active stage was higher than that of stable and regular patients (68.26167.64). The difference was statistically significant (p=0.024) in patients with.4 before uveitis, serum IL-16 level 84.55 (51.39127.04) was significantly lower than 196.42 (140.77263.26) before treatment (p=), and the difference was statistically significant (p=). 0.004); and the serum IL-32 level 88.98 (52.17165.43) after the treatment of anterior uveitis was also significantly lower than that before treatment (214.51342.82), and the difference was also statistically significant (p=0.000).5 with Spearman correlation analysis of IL-16 and IL-32 and anterior uveitis. The results showed that IL-16 was positively related to the disease (Spearman correlation). The coefficient r=0.671, p=0.000) and IL-32 were also positively correlated with anterior uveitis (Spearman correlation coefficient r=0.963, p=0.000).Il-16 and IL-32 (Spearman correlation coefficient r=0.383, p=0.009) before.6 anterior uveitis. The results were 24.50, 6.30, 64.10, and 4.80 respectively. (4.02,5.47); the results of ESR, RF, CRP and anti chain "O" in the normal control group were 15.60 (6.90,27.70), 4.52 (3.07,8.35), 47.50 (8.10,95.80) and 3.43 (2.65,4.12). The ESR level of the anterior uveitis group was higher than that in the normal control group, and the difference was statistically significant (P=0.004). There was no statistical difference between the two groups. Meaning (RF:P=0.645; CRP:P=0.053; anti chain "O": P=0.620).7 with Spearman correlation analysis of the correlation between IL-16, IL-32 and the patients with anterior uveitis, ESR, RF, CRP and anti chain O. Conclusion: the 1 studies found that uveitis is in middle green. The incidence of anterior uveitis is common in the population. The common complications of anterior uveitis are cataract and glaucoma or intraocular pressure. Most of the patients have better visual prognosis. There are few visual impairment in.2 anterior uveitis patients' serum IL-16, and the level of IL-32 is significantly higher than that in the normal control group; IL-16, IL-32 is positive with the disease, respectively. Correlation, confirmed that IL-16, IL-32 is an important cytokine in the anterior uveitis, indicating that IL-16, IL-32 participates in the serum IL-16 in the patients with anterior uveitis during the onset of anterior uveitis, and the level of IL-32 is significantly higher than that in the stable stage, which confirms that IL-16, IL-32 and the activity of anterior uveitis are related to the serum IL of the patients with.4 anterior uveitis after treatment. -16, IL-32 level was significantly lower than before treatment, and further confirmed IL-16, IL-32 participated in the occurrence and development of anterior uveitis,.5 serum was positively correlated with IL-16 and IL-32, which was considered to be involved in the occurrence and evolution of anterior uveitis respectively.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R773
本文编号：2139896
[Abstract]:Objective: 1 to observe the clinical characteristics, therapeutic effect, visual prognosis, complications, and problems in the diagnosis and treatment of uveitis in this study,.2 detection of the expression of IL-16 and IL-32 in the serum of normal people and patients with anterior uveitis, and to compare the differences of IL-16 and IL-32 expression levels between groups and analyze them with the disease, respectively. The correlation.3 detected the expression of IL-16 and IL-32 in the sera of the active and stable patients with anterior uveitis, compared with the expression level, and detected the expression of IL-16 and IL-32 in the sera before and after the treatment of the patients with anterior uveitis. There was no significant difference before and after the treatment, and the relationship between them and the disease was further analyzed. .4 was used to explore the role of IL-16 and IL-32 in the pathogenesis of anterior uveitis, looking for new methods and new drugs for the treatment of pre uveitis. 1 a total of 85 cases of uveitis were collected from January 2015 to January 2017 in the first hospital of Hebei Medical University, including 45 males and 40 females, with an average age of onset (41.25 + 16.83), and the age range of onset. At the age of 7-70, the whole body condition, the history of ophthalmopathy and the clinical diagnosis and treatment were recorded in detail. 40 cases of non uveitis were collected in the same period as the normal control group, including 22 males and 18 females, with an average age of 42.33 + 12.68. All the controls excluded the history of uveitis, previous body health, no antibiotics, glucocorticoids, and non steroids. The body hormone and immunosuppressant.2 collected 40 cases of anterior uveitis and 40 cases of normal control group of peripheral venous blood samples 4-6ml, extracted serum after centrifugation, and then used the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the expression of IL-16 and IL-32 in the serum of the experimental group and the normal control group. The test group and the normal control group were Erythrocyte Sedimentation Rate (ESR), rheumatoid factor (Rheumatoid factor, RF), C reactive protein (C-Reactive protein, CRP) and anti chain O). The results were statistically analyzed. Results: 1 of the 85 cases of uveitis were the most 21-45 years old patients and age model. The peri was 7-70 years old, with a male and female ratio of 1.125:1. with 67 cases of monocular disease and 18 cases with double binocular involvement. According to the anatomical site, anterior uveitis (64 cases) was the most, followed by full uveitis in 13 cases, middle uveitis and 4 cases of posterior uveitis, according to the course of the disease (61 cases), the most, chronic uveitis There were 5 patients and 19 cases of recurrent uveitis, including 7 cases with ankylosing spondylitis, 6 cases (7.9%) with vogt- Xiao Liu Tian (VKH) syndrome, 5 cases with Behcet disease and 4 cases of Fuchs syndrome. In this study, 85 cases of the study included cataract, glaucoma or intraocular pressure, and synechia of the iris. The visual acuity of most patients after timely active drug treatment improved the concentration of IL-16 and IL-32 in the serum of.2 anterior uveitis group was 196.42 (140.77263.26) and 265.67 (214.51342.82), and the serum concentration of IL-16 and IL-32 in the normal control group was 82.68 (49.03115.38) and 86.19 (51.57154.28), respectively. The serum IL-16 concentration was significantly different from that of the normal control group (p=0.003). The serum IL-32 concentration in the anterior uveitis group was also significantly different from that of the normal control group (p=0.008) the serum IL-16 level 209.55 (152.89277.40) was higher than the stable level 97.19 (57.92123.17) in the patients with.3 anterior uveitis. Study significance (p=0.000); serum IL-32 level 278.98 (222.12346.80) of patients at active stage was higher than that of stable and regular patients (68.26167.64). The difference was statistically significant (p=0.024) in patients with.4 before uveitis, serum IL-16 level 84.55 (51.39127.04) was significantly lower than 196.42 (140.77263.26) before treatment (p=), and the difference was statistically significant (p=). 0.004); and the serum IL-32 level 88.98 (52.17165.43) after the treatment of anterior uveitis was also significantly lower than that before treatment (214.51342.82), and the difference was also statistically significant (p=0.000).5 with Spearman correlation analysis of IL-16 and IL-32 and anterior uveitis. The results showed that IL-16 was positively related to the disease (Spearman correlation). The coefficient r=0.671, p=0.000) and IL-32 were also positively correlated with anterior uveitis (Spearman correlation coefficient r=0.963, p=0.000).Il-16 and IL-32 (Spearman correlation coefficient r=0.383, p=0.009) before.6 anterior uveitis. The results were 24.50, 6.30, 64.10, and 4.80 respectively. (4.02,5.47); the results of ESR, RF, CRP and anti chain "O" in the normal control group were 15.60 (6.90,27.70), 4.52 (3.07,8.35), 47.50 (8.10,95.80) and 3.43 (2.65,4.12). The ESR level of the anterior uveitis group was higher than that in the normal control group, and the difference was statistically significant (P=0.004). There was no statistical difference between the two groups. Meaning (RF:P=0.645; CRP:P=0.053; anti chain "O": P=0.620).7 with Spearman correlation analysis of the correlation between IL-16, IL-32 and the patients with anterior uveitis, ESR, RF, CRP and anti chain O. Conclusion: the 1 studies found that uveitis is in middle green. The incidence of anterior uveitis is common in the population. The common complications of anterior uveitis are cataract and glaucoma or intraocular pressure. Most of the patients have better visual prognosis. There are few visual impairment in.2 anterior uveitis patients' serum IL-16, and the level of IL-32 is significantly higher than that in the normal control group; IL-16, IL-32 is positive with the disease, respectively. Correlation, confirmed that IL-16, IL-32 is an important cytokine in the anterior uveitis, indicating that IL-16, IL-32 participates in the serum IL-16 in the patients with anterior uveitis during the onset of anterior uveitis, and the level of IL-32 is significantly higher than that in the stable stage, which confirms that IL-16, IL-32 and the activity of anterior uveitis are related to the serum IL of the patients with.4 anterior uveitis after treatment. -16, IL-32 level was significantly lower than before treatment, and further confirmed IL-16, IL-32 participated in the occurrence and development of anterior uveitis,.5 serum was positively correlated with IL-16 and IL-32, which was considered to be involved in the occurrence and evolution of anterior uveitis respectively.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R773
【参考文献】
相关期刊论文 前7条
1 陆晓雅;王毓琴;郑美琴;徐一;楼永良;;650例前葡萄膜炎临床分析[J];中华眼视光学与视觉科学杂志;2013年12期
2 曾庆理;李少丽;周燕斌;;白细胞介素-32在炎症与肿瘤中的研究进展[J];新医学;2013年11期
3 吴松明;朱兴全;袁子国;;白细胞介素-16的研究进展[J];中国畜牧兽医;2011年05期
4 郑曰忠;时冀川;;前葡萄膜炎的临床诊治分析[J];中华眼科杂志;2010年01期
5 高燕;胡绍胜;郭洪志;;多发性硬化与免疫研究新进展[J];实用医技杂志;2007年08期
6 唐先梅;刘兰;刘佩芝;季榕;;白细胞介素16及其与临床疾病的关系[J];临床医学;2006年08期
7 王深明;;甲状腺功能亢进的分类和病因[J];中国实用外科杂志;2006年07期
，本文编号：2139896
本文链接：https://www.wllwen.com/yixuelunwen/wuguanyixuelunwen/2139896.html
最近更新
教材专著
