汉黄芩素体外抗人喉癌Hep2细胞增殖、凋亡和侵袭的作用及机制研究

发布时间：2018-07-25 08:21

【摘要】：目的研究汉黄芩素对人喉癌Hep-2细胞增殖、凋亡及侵袭能力的影响,并探讨其作用机制。方法运用CCK-8检测汉黄芩素对人喉癌Hep-2细胞增殖活性的影响。运用流式细胞仪检测汉黄芩素对人喉癌Hep-2细胞凋亡的影响。运用Hoechst染色法检测汉黄芩素对人喉癌Hep-2细胞核形态变化的影响。运用transwell侵袭实验比较汉黄芩素对人喉癌Hep-2细胞侵袭能力的影响。运用Western blot检测汉黄芩素对人喉癌Hep-2细胞h TERT、Bcl-2、Bax蛋白表达水平的影响。结果 CCK-8法发现汉黄芩素能抑制人喉癌Hep-2细胞增殖,其作用呈浓度依赖性。流式细胞术分析发现汉黄芩素能诱导人喉癌Hep-2细胞发生凋亡。Hoechst染色发现汉黄芩素能诱导人喉癌Hep-2细胞核形态变化。Transwell实验发现汉黄芩素能抑制人喉癌Hep-2细胞的体外侵袭能力。Western blot检测发现测汉黄芩素能有效降低人喉癌Hep-2细胞h TERT、Bcl-2蛋白表达水平以及增加Bax蛋白表达水平(P0.05)。结论汉黄芩素在体外能够有效抑制人喉癌Hep-2细胞增殖,诱导细胞凋亡,同时抑制喉癌细胞的体外侵袭能力。其作用可能与调控Bcl-2蛋白家族及h TERT蛋白表达水平相关。
[Abstract]:Objective to study the effects of baicalin on proliferation, apoptosis and invasion of human laryngeal carcinoma Hep-2 cells and its mechanism. Methods the effect of baicalin on the proliferation of human laryngeal carcinoma Hep-2 cells was detected by CCK-8. The effect of baicalin on apoptosis of human laryngeal carcinoma Hep-2 cells was detected by flow cytometry. The effects of baicalin on the nuclear morphologic changes of Hep-2 in human laryngeal carcinoma were detected by Hoechst staining. Transwell invasion assay was used to compare the effect of baicalin on the invasion ability of human laryngeal carcinoma Hep-2 cells. The effect of baicalin on the expression of hTERTnBcl-2mBax protein in human laryngeal carcinoma Hep-2 cells was detected by Western blot. Results CCK-8 assay showed that baicalin could inhibit the proliferation of human laryngeal carcinoma Hep-2 cells in a concentration dependent manner. Flow cytometric analysis showed that baicalin could induce apoptosis of human laryngeal carcinoma Hep-2 cells. Hoechst staining found that Chinese baicalin could induce nuclear morphologic changes of human laryngeal carcinoma Hep-2. Transwell assay found that Chinese baicalin could inhibit human laryngeal cancer Hep-2 cells in vitro. Western blot assay showed that baicalin could effectively decrease the expression of hTERTnBcl-2 protein and increase the expression of Bax protein in human laryngeal carcinoma Hep-2 cells (P0.05). Conclusion baicalin can effectively inhibit the proliferation and apoptosis of human laryngeal carcinoma Hep-2 cells in vitro and inhibit the invasiveness of human laryngeal carcinoma cells in vitro. Its role may be related to the regulation of Bcl-2 protein family and the expression of h TERT protein.
【作者单位】： 湖北省郧西县人民医院耳鼻咽喉科;湖北省郧西县人民医院神经内科;
【分类号】：R739.65

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