息肉状脉络膜血管病变血清药物靶向因子的初步筛选
发布时间：2018-08-06 15:17
【摘要】：目的利用蛋白芯片技术筛选息肉状脉络膜(PCV)血清差异性蛋白,以便寻找PCV的早期诊断标志物和药物治疗新靶点,为进一步探讨PCV发病机制提供依据。方法1.用AAH-BLG-507蛋白芯片(含507种功能蛋白)检测随机抽取的1例阴性对照组、1例AMD患者及2例PCV患者血清中相应蛋白的表达水平,分析差异性表达蛋白。2.根据AAH-BLG-507蛋白芯片结果,选取相关细胞因子和趋化因子构建成含34种因子的多因子检测试剂盒(即液相芯片),检测65例PCV患者(男性42例,女性23例;年龄分布在49岁到81岁之间)和70例阴性对照组(男性40例,女性30例;年龄分布在53岁到78岁之间)血清中相关34种因子的表达,筛选有差异性表达的因子。结果1.入组的PCV患者平均年龄(65.43±8.57)岁,与对照组相比无显著差异(P=0.070.05)。两组性别构成比较,结果显示无显著差异(P0.05),认为两组患者年龄和性别匹配。2.AAH-BLG-507蛋白芯片检测结果分析显示,有79种蛋白表达有差异,其中58种在PCV患者血清中呈上调趋势,21种呈下调趋势。分析表达差异性蛋白之间关系,结果显示主要分为细胞因子、趋化因子、胞外基质蛋白/基质金属蛋白酶、生长因子及新生血管相关因子及其受体4大类。3.多因子检测试剂盒结果显示,选取的34种因子中有10种有统计学差异(P0.05)其中8种因子及其在PCV血清中对应的浓度:Eotaxin(52.618±24.954)pg/mL,IL-1RA(289.232±146.678)pg/m L;IL-1β(0.481±0.279)pg/mL、IL-4(2.069±1.050)pg/mL、IL-5(0.169±0.111)pg/mL、IL-13(0.424±0.122)pg/mL、IL-22(4.730±3.790)pg/m L、GM-CSF(1.542±0.752)pg/mL,明显高于其在对照组血清浓度,即这8种因子在PCV患者血清中呈上调趋势(P值分别为0.003、0.007、0.009、0.002、0.040、0.003、0.003、0.003均0.05)。而另外2种因子在PCV患者血清中浓度IL-7(2.929±2.749)pg/mL、SDF-1α(347.141±101.243)pg/m L明显低于对照组,即呈下调趋势(P值分别为0.003和0.008均0.05)。结论1.蛋白芯片技术是一种快速、简便的高通量蛋白质组研究方法,为PCV研究提供了新的研究策略。2.PCV的发病可能与患者血清中Eotaxin、IL-1RA、GM-CSF、IL-1β、IL-4、IL-5、IL-13、IL-22表达上调和IL-7、SDF-1α表达下调相关。3.筛选出的PCV相关的差异蛋白,将可能作为PCV患者的诊断分子标志物和药物治疗靶向因子。
[Abstract]:Objective to screen serum differential proteins of polypoid choroidal (PCV) by using protein chip technique in order to search for early diagnostic markers and new targets of drug therapy for PCV and to provide evidence for further study of pathogenesis of PCV. Method 1. The expression levels of the corresponding proteins in the serum of 1 AMD patient and 2 PCV patients were detected by AAH-BLG-507 protein chip (including 507 functional proteins), and the differentially expressed protein. 2 was analyzed. According to the results of AAH-BLG-507 protein chip, 34 cytokines and chemokines were selected to construct a multifactor detection kit (liquid chip) containing 34 factors. 65 patients with PCV were detected (42 males and 23 females). Age ranged from 49 to 81 years old) and 70 cases of negative control group (male 40, female 30; age between 53 and 78 years old) had 34 related factors. Result 1. The mean age of the patients with PCV was (65.43 卤8.57) years old, which was not significantly different from that of the control group (P0.070.05). The results showed that there was no significant difference between the two groups (P0.05). It was considered that the age and sex matched. 2.AAH-BLG-507 protein chip analysis showed that there were 79 protein expression differences between the two groups. 58 of them were up-regulated in serum of PCV patients and 21 were down-regulated. The expression of differentially expressed proteins was analyzed. The results showed that they were divided into four categories: cytokines, chemokines, extracellular matrix proteins / matrix metalloproteinases, growth factors and neovascularization related factors and their receptors. 澶氬洜瀛愭娴嬭瘯鍓傜洅缁撴灉鏄剧ず,閫夊彇鐨，
本文编号：2168130
[Abstract]:Objective to screen serum differential proteins of polypoid choroidal (PCV) by using protein chip technique in order to search for early diagnostic markers and new targets of drug therapy for PCV and to provide evidence for further study of pathogenesis of PCV. Method 1. The expression levels of the corresponding proteins in the serum of 1 AMD patient and 2 PCV patients were detected by AAH-BLG-507 protein chip (including 507 functional proteins), and the differentially expressed protein. 2 was analyzed. According to the results of AAH-BLG-507 protein chip, 34 cytokines and chemokines were selected to construct a multifactor detection kit (liquid chip) containing 34 factors. 65 patients with PCV were detected (42 males and 23 females). Age ranged from 49 to 81 years old) and 70 cases of negative control group (male 40, female 30; age between 53 and 78 years old) had 34 related factors. Result 1. The mean age of the patients with PCV was (65.43 卤8.57) years old, which was not significantly different from that of the control group (P0.070.05). The results showed that there was no significant difference between the two groups (P0.05). It was considered that the age and sex matched. 2.AAH-BLG-507 protein chip analysis showed that there were 79 protein expression differences between the two groups. 58 of them were up-regulated in serum of PCV patients and 21 were down-regulated. The expression of differentially expressed proteins was analyzed. The results showed that they were divided into four categories: cytokines, chemokines, extracellular matrix proteins / matrix metalloproteinases, growth factors and neovascularization related factors and their receptors. 澶氬洜瀛愭娴嬭瘯鍓傜洅缁撴灉鏄剧ず,閫夊彇鐨，
本文编号：2168130
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