内质网应激相关因子caspase-12在糖尿病大鼠视网膜的表达
发布时间：2018-08-14 12:27
【摘要】：目的检测半胱天冬酶12(caspase-12)在糖尿病(DM)大鼠视网膜的表达情况,探讨内质网应激在糖尿病视网膜病变(DR)中的作用。方法健康雄性SD大鼠30只随机分成空白组(CON组)15只,糖尿病1月组(DM1组)、糖尿病2月组(DM2组)、糖尿病3月组(DM3组),每组5只。分别于造模后1,2,3个月摘除眼球,行HE染色、免疫组织化学染色,观察视网膜形态,计数视网膜神经节细胞(RCGs),并检测caspase-12在视网膜的表达情况。结果视网膜HE染色显示DM组大鼠视网膜神经节细胞减少,内外核层细胞稀疏,排列紊乱,网状层空泡样变性等改变,且随病程延长逐渐加重。RCGs计数结果 CON组、DM1组、DM2组、DM3组结果分别是281.20±7.98,263.60±13.37,240.60±11.78,194.80±13.16,各组组间差异均有统计学意义(P0.05)。免疫组织化学染色显示,caspase-12在CON组没有阳性表达,在DM组主要表达于神经节细胞层。随着病程进展,内核层也有表达,且表达强度逐渐增强。DM1组、DM2组、DM3组caspase-12表达的平均光密度值(IOD/area)分别是0.0173±0.0039,0.0348±0.0050,0.0579±0.0074,DM2组和DM3组与DM1组比较差异有统计学意义(P0.01)。结论随着DM病程进展,DR大鼠RGCs凋亡逐渐增加,caspase-12诱导的内质网凋亡在DR发病中有重要作用,内质网应激促进了DR的发生、发展。
[Abstract]:Objective to detect the expression of cysteinase 12 (caspase-12) in the retina of diabetic (DM) rats and to explore the role of endoplasmic reticulum stress in diabetic retinopathy (DR). Methods Thirty healthy male Sprague-Dawley rats were randomly divided into control group (CON group, n = 15), diabetic group (DM1 group, n = 15), diabetic group (DM2 group, n = 5) and diabetic group (DM3 group, n = 5). The eyeballs were removed at 1 ~ 2 and 3 months after the model. The retinal morphology was observed by HE staining and immunohistochemical staining. The (RCGs), of retinal ganglion cells was counted and the expression of caspase-12 in the retina was detected. Results Retinal HE staining showed that the retinal ganglion cells were decreased, the cells in the inner and outer nuclear layers were sparse, the arrangement was disordered, and the vacuolar degeneration of the reticular layer was changed in DM group. The results of RCGs count in CON group were 281.20 卤7.98263.60 卤13.37240.60 卤11.78194.80 卤13.16, respectively (P0.05). Immunohistochemical staining showed that caspase-12 was not expressed in CON group, but mainly in ganglion cell layer in DM group. With the progression of the disease, the expression of caspase-12 was also found in the kernel layer, and the average optical density (IOD/area) of caspase-12 expression in DM2 group was 0.0173 卤0.0039 卤0.0050 卤0.054 卤0.0579 卤0.0074 in DM2 group and DM3 group was significantly higher than that in DM1 group (P0.01). Conclusion the apoptosis of endoplasmic reticulum induced by caspase-12 plays an important role in the pathogenesis of Dr with the progression of DM. Endoplasmic reticulum stress promotes the occurrence and development of Dr.
【作者单位】： 潍坊医学院眼科学教研室;潍坊医学院附属医院眼科;
【基金】：山东省教育厅资助课题(课题编号:J10LF6)
【分类号】：R587.1;R774.1
，
本文编号：2182860
[Abstract]:Objective to detect the expression of cysteinase 12 (caspase-12) in the retina of diabetic (DM) rats and to explore the role of endoplasmic reticulum stress in diabetic retinopathy (DR). Methods Thirty healthy male Sprague-Dawley rats were randomly divided into control group (CON group, n = 15), diabetic group (DM1 group, n = 15), diabetic group (DM2 group, n = 5) and diabetic group (DM3 group, n = 5). The eyeballs were removed at 1 ~ 2 and 3 months after the model. The retinal morphology was observed by HE staining and immunohistochemical staining. The (RCGs), of retinal ganglion cells was counted and the expression of caspase-12 in the retina was detected. Results Retinal HE staining showed that the retinal ganglion cells were decreased, the cells in the inner and outer nuclear layers were sparse, the arrangement was disordered, and the vacuolar degeneration of the reticular layer was changed in DM group. The results of RCGs count in CON group were 281.20 卤7.98263.60 卤13.37240.60 卤11.78194.80 卤13.16, respectively (P0.05). Immunohistochemical staining showed that caspase-12 was not expressed in CON group, but mainly in ganglion cell layer in DM group. With the progression of the disease, the expression of caspase-12 was also found in the kernel layer, and the average optical density (IOD/area) of caspase-12 expression in DM2 group was 0.0173 卤0.0039 卤0.0050 卤0.054 卤0.0579 卤0.0074 in DM2 group and DM3 group was significantly higher than that in DM1 group (P0.01). Conclusion the apoptosis of endoplasmic reticulum induced by caspase-12 plays an important role in the pathogenesis of Dr with the progression of DM. Endoplasmic reticulum stress promotes the occurrence and development of Dr.
【作者单位】： 潍坊医学院眼科学教研室;潍坊医学院附属医院眼科;
【基金】：山东省教育厅资助课题(课题编号:J10LF6)
【分类号】：R587.1;R774.1
，
本文编号：2182860
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