甲状腺乳头状癌与喉鳞状细胞癌代谢组学初步研究

发布时间：2018-08-20 11:28

【摘要】：目的:阐明甲状腺乳头状癌(PTC)与喉鳞状细胞癌(LSCC)的代谢组学特征并探讨其异同点。方法:联合应用气相色谱-飞行时间质谱和超高效液相色谱-飞行时间质谱技术,对57例PTC和33例LSCC患者的新鲜肿瘤组织及其邻近非肿瘤组织的代谢物进行检测,获取代谢谱。应用单维、多维统计学方法分析肿瘤组织与邻近非肿瘤组织之间的差异代谢产物以及相关的代谢通路。结果:PTC以及LSCC患者肿瘤组织与邻近非肿瘤组织分别存在46及41种差异代谢产物。两组患者共同的代谢特征主要表现在肿瘤组织中糖酵解、氨基酸代谢、一碳代谢及色氨酸代谢均较邻近非肿瘤组织明显增强。PTC与LSCC肿瘤组织中的嘌呤和嘧啶代谢产物较邻近非肿瘤组织增多,牛磺酸和次牛磺酸的代谢产物在PTC肿瘤组织中增多。脂肪酸代谢产物在PTC以及LSCC中又呈现共性的降低。结论:PTC与LSCC既具有肿瘤组织中糖酵解、氨基酸代谢、一碳代谢及色氨酸代谢明显增强的共性代谢特点,又具有各自独特的代谢特征,这些特征的揭示有助于阐明上述肿瘤的生物学特征。
[Abstract]:Objective: to elucidate the metabolic characteristics of (PTC) and (LSCC) in papillary thyroid carcinoma and laryngeal squamous cell carcinoma. Methods: gas chromatography-time-of-flight mass spectrometry and ultra-high performance liquid chromatography-time-of-flight mass spectrometry were used to detect the metabolites of fresh tumor tissues and their adjacent non-tumor tissues in 57 patients with PTC and 33 patients with LSCC. The differential metabolites and related metabolic pathways between tumor tissues and adjacent nonneoplastic tissues were analyzed by single dimensional and multidimensional statistical methods. Results there were 46 and 41 different metabolites in the tumor tissues of the patients with LSCC and those in the adjacent non-tumor tissues. The common metabolic characteristics of the two groups were mainly the glycolysis and amino acid metabolism in tumor tissues. The metabolites of purine and pyrimidine in the tumor tissues of LSCC and PTC were higher than those in the nonneoplastic tissues of PTC, and the metabolites of taurine and subtaurine were increased in the tumor tissues of PTC. Fatty acid metabolites decreased in general in PTC and LSCC. Conclusion LSCC and LSCC not only have the common metabolic characteristics of glycolysis, amino acid metabolism, monocarbon metabolism and tryptophan metabolism, but also have their own unique metabolic characteristics. The revelation of these features helps to clarify the biological characteristics of these tumors.
【作者单位】：上海交通大学医学院附属仁济医院头颈外科;
【基金】：上海高校选拔培养优秀青年教师科研专项基金(No:101005.001.14.115)
【分类号】：R736.1;R739.65

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