IRX3在正常角结膜以及翼状胬肉上皮中表达的研究

发布时间：2018-09-08 14:08

【摘要】：目的：以往的研究发现IRX3在眼前节发育中发挥着重要作用，然而关于IRX3在正常成人和小鼠眼表上皮表达的研究报道甚少，本研究旨在观察IRX3在正常成年人和小鼠的角膜上皮中的表达情况，以及探究IRX3在正常成人结膜与翼状胬肉上皮中的表达差异。方法：采用免疫荧光和免疫组织化学染色技术观察IRX3在正常小鼠和人角膜上皮的表达情况。采用western blotting对比研究正常人角膜缘与中央角膜上皮IRX3的表达情况。采用免疫荧光技术对比观察IRX3在正常结膜与翼状胬肉上皮中的表达情况，，并采用western blotting半定量对比研究其表达情况。结果：IRX3在正常小鼠和人角膜上皮存在表达，IRX3在正常人角膜缘的表达高于中央角膜上皮；正常结膜上皮中几乎无IRX3的表达，而其在翼状胬肉上皮中的表达明显升高。结论：IRX3在正常小鼠和人角膜上皮有表达，且正常人角膜缘的表达高于中央角膜上皮；而正常结膜上皮中IRX3几乎无表达，IRX3在翼状胬肉上皮中的表达较正常结膜上皮明显升高。
[Abstract]:Objective: previous studies have found that IRX3 plays an important role in the development of anterior segment, but there are few reports on the expression of IRX3 in normal adult and mouse ocular surface epithelium. The purpose of this study was to observe the expression of IRX3 in corneal epithelium of normal adults and mice and to explore the difference of IRX3 expression between conjunctiva and pterygium epithelium of normal adults. Methods: immunofluorescence and immunohistochemical staining were used to observe the expression of IRX3 in normal mice and human corneal epithelium. The expression of IRX3 in limbus cornea and central corneal epithelium was studied by western blotting. The expression of IRX3 in normal conjunctiva and pterygium epithelium was observed by immunofluorescence technique and semi-quantitative western blotting was used to study its expression. Results the expression of IRX3 in normal mice and human corneal epithelium was higher than that in central cornea epithelium, and there was almost no IRX3 expression in normal conjunctiva epithelium, but the expression of IRX3 in pterygium epithelium was significantly increased. Conclusion the expression of IRX3 IRX3 in normal mouse and human corneal epithelium is higher than that in central corneal epithelium, and the expression of IRX3 in normal conjunctiva epithelium is higher than that in normal conjunctival epithelium.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R777.33

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