JAK2-STAT3基因多态与Bebcet病和Vogt-小柳原田综合征遗传易感性研究
发布时间：2018-09-10 12:37
【摘要】：目的：蛋白酪氨酸激酶2-信号转导和转录活化因子3(JAK2-STAT3)所构成的通路广泛参与细胞增殖、分化、以及免疫调节等过程，是IL-12，IL-23等细胞因子信号转导的重要途径。最近的研究发现多发性硬化、溃疡性结肠炎和强制性脊柱炎等多个自身免疫病的发生与JAK2-STAT3基因多态相关。由此本研究旨在探讨同为自身免疫性疾病的Behcet病和Vogt-小柳原田综合征在中国汉族患者中是否与JAK2-STAT3基因多态相关。 方法: PCR-RFLP、PCR-SSCP方法对Behcet患者、Vogt-小柳原田综合征患者和615例正常对照JAK2基因三个多态位点，STAT3基因四个多态位点进行基因分型。 结果: Behcet患者STAT3基因rs2293152的GG基因型频率显著高于正常对照（P=0.001,校正P=0.021）。STAT3基因多态与Vogt-小柳原田综合征无关，JAK2基因多态与Behcet病和Vogt-小柳原田综合征均无显著相关性。 结论: STAT3基因rs2293152的GG基因型与Behcet病患者相关，，JAK2基因多态与Behcet病和Vogt-小柳原田综合征均无相关性。
[Abstract]:Objective: protein tyrosine kinase 2-signal transduction and transcriptional activator 3 (JAK2-STAT3) pathway are involved in the process of cell proliferation, differentiation and immunomodulation, which are important pathways of IL-12,IL-23 and other cytokines signal transduction. Recent studies have found that multiple autoimmune diseases, such as multiple sclerosis, ulcerative colitis and mandatory spondylitis, are associated with JAK2-STAT3 gene polymorphism. The purpose of this study was to investigate the association of JAK2-STAT3 gene polymorphism with Behcet's disease and Vogt- Xiaoliu Harada syndrome in Chinese Han nationality. Methods: three polymorphic loci of JAK2 gene and four loci of STAT3 gene were genotyped by PCR-RFLP,PCR-SSCP in Behcet patients with Vogt- willow Harada syndrome and 615 normal controls. Results: the frequency of GG genotype of STAT3 gene rs2293152 in Behcet patients was significantly higher than that of normal controls (P0. 001, corrected P0. 021) .STAT3 gene polymorphism was not associated with Vogt- willow Harada syndrome. There was no significant correlation between JAK2 gene polymorphism and Behcet disease and Vogt- Xiaoyanagata syndrome. Conclusion: the GG genotype of STAT3 gene rs2293152 is not correlated with Behcet disease and Vogt- willow Harada syndrome.
【学位授予单位】：重庆医科大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R773.9
本文编号：2234467
[Abstract]:Objective: protein tyrosine kinase 2-signal transduction and transcriptional activator 3 (JAK2-STAT3) pathway are involved in the process of cell proliferation, differentiation and immunomodulation, which are important pathways of IL-12,IL-23 and other cytokines signal transduction. Recent studies have found that multiple autoimmune diseases, such as multiple sclerosis, ulcerative colitis and mandatory spondylitis, are associated with JAK2-STAT3 gene polymorphism. The purpose of this study was to investigate the association of JAK2-STAT3 gene polymorphism with Behcet's disease and Vogt- Xiaoliu Harada syndrome in Chinese Han nationality. Methods: three polymorphic loci of JAK2 gene and four loci of STAT3 gene were genotyped by PCR-RFLP,PCR-SSCP in Behcet patients with Vogt- willow Harada syndrome and 615 normal controls. Results: the frequency of GG genotype of STAT3 gene rs2293152 in Behcet patients was significantly higher than that of normal controls (P0. 001, corrected P0. 021) .STAT3 gene polymorphism was not associated with Vogt- willow Harada syndrome. There was no significant correlation between JAK2 gene polymorphism and Behcet disease and Vogt- Xiaoyanagata syndrome. Conclusion: the GG genotype of STAT3 gene rs2293152 is not correlated with Behcet disease and Vogt- willow Harada syndrome.
【学位授予单位】：重庆医科大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R773.9
【参考文献】
相关期刊论文 前3条
1 杨培增;龚向明;;葡萄膜炎在世界各地的发生及研究概况——亚洲[J];国外医学.眼科学分册;1992年05期
2 马文芝,蒋黎人;妊娠高血压综合征眼底观察[J];实用眼科杂志;1986年05期
3 张振宇,刘英奇;妊娠高血压综合征眼底改变分析及新分级法的建议[J];实用眼科杂志;1987年04期
本文编号：2234467
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