氧化损伤对耳蜗毛细胞Caspase-3和Caspase-9蛋白表达水平的影响

发布时间：2018-09-17 08:11

【摘要】：为研究氧化应激损伤耳蜗毛细胞Caspase-3、Caspase-9蛋白的表达水平,以探讨氧化应激诱发毛细胞凋亡的机制,将处于对数生长期的耳蜗毛细胞(HEI-OC1细胞)分别暴露于含终浓度为0(对照)、50、100、200μmol/L过氧化氢的DMEM高糖培养基24 h,以构建毛细胞氧化应激损伤模型。检测毛细胞内丙二醛(MDA)含量及Caspase-3和Caspase-9蛋白的表达水平。结果显示,与对照组比较,各浓度过氧化氢染毒组HEI-OC1细胞内MDA的含量均增高,差异有统计学意义(P0.01);且随着过氧化氢染毒浓度的升高,HEI-OC1细胞内MDA的含量呈上升趋势。与对照组比较,各浓度过氧化氢染毒组HEI-OC1细胞内Caspase-3和Caspase-9蛋白的表达水平均增高,差异有统计学意义(P0.05);且随着过氧化氢染毒浓度的升高,HEI-OC1细胞内Caspase-3和Caspase-9蛋白的表达水平均呈先上升后下降的趋势。提示氧化应激损伤可诱导耳蜗毛细胞的凋亡,但凋亡水平随氧化应激损伤程度的加重而呈先升高后下降的趋势。
[Abstract]:To study the expression of Caspase-3,Caspase-9 protein in cochlear hair cells after oxidative stress injury, and to explore the mechanism of oxidative stress inducing hair cell apoptosis. Cochlear hair cells (HEI-OC1 cells) at logarithmic growth stage were exposed to high glucose DMEM medium containing 0 (control) 50100200 渭 mol/L hydrogen peroxide for 24 h to establish oxidative stress injury model of hair cells. The content of malondialdehyde (MDA) and the expression of Caspase-3 and Caspase-9 in hair cells were measured. The results showed that compared with the control group, the content of MDA in HEI-OC1 cells was significantly higher in different concentrations of hydrogen peroxide (P0.01), and the content of MDA in HEI-OC1 cells increased with the increase of concentration of hydrogen peroxide (P0.01). Compared with the control group, the expression levels of Caspase-3 and Caspase-9 protein in HEI-OC1 cells were increased in the groups exposed to hydrogen peroxide at different concentrations. The difference was statistically significant (P0.05), and the expression of Caspase-3 and Caspase-9 in HEI-OC1 cells increased first and then decreased with the increase of hydrogen peroxide concentration. It was suggested that oxidative stress injury could induce apoptosis of cochlear hair cells, but the level of apoptosis increased first and then decreased with the severity of oxidative stress injury.
【作者单位】：广东药科大学公共卫生学院预防医学系;广东药科大学公共卫生学院劳动卫生与环境卫生学系;
【基金】：广东大学生科技创新培育专项(pdjh2016b0258) 广东省科技计划项目(2014A020212601)
【分类号】：R764

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