去甲斑蝥酸钠对鼻咽癌CNE-2细胞株体外放射敏感性的影响

发布时间：2018-10-10 14:03

【摘要】：目的本实验旨在探讨去甲斑蝥酸钠(Disodium Cantharidinate)对鼻咽癌CNE-2细胞株体外的放射增敏作用,并初步探讨其机理。方法应用MTS法测定不同时间、不同浓度去甲斑蝥素钠对鼻咽癌CNE-2细胞的增殖抑制作用,求出半数抑制浓度(50%Inhibiting Concentration, IC50)。将鼻咽癌CNE-2细胞分为：空白对照组、照射组、药物组、药物与照射联合组,应用克隆形成法研究去甲斑蝥酸钠与放射联合效应,利用“多靶单击”数学模型[SF=1-(1-e-D/D0)ΔN进行曲线拟合,获得细胞存活曲线及平均致死剂量、准阈剂量、放射增敏比(Sensitization enhancement ratio, SER)等参数,进行效应评估。将鼻咽癌CNE-2细胞分为：空白对照组、照射组、药物组、药物与照射联合组,去甲斑蝥酸钠作用细胞48h后,流式细胞术分析细胞凋亡率及周期的变化。结果去甲斑蝥酸钠作用后鼻咽癌CNE-2细胞生长增殖受到抑制,作用48小时IC50为340mg/L。20mg/L去甲斑蝥酸钠作用鼻咽癌CNE-2细胞48h后联合放射的放射增敏比(SER)为1.43。去甲斑蝥酸钠与放疗作用于CNE-2细胞株后流式细胞仪检测细胞周期,G2/M期细胞急剧增高,由8.26%上升至48.3%,G1和S期细胞明显减少。CNE-2细胞经4Gy照射、20mg/L去甲斑蝥酸钠处理后,凋亡率较空白对照组细胞升高,分别为12.68%,5.37%,而照射联合去甲斑蝥酸钠处理组中,凋亡率升高更为显著,可达18.84%。比较差异均有统计学意义(P0.05)。结论 1、去甲斑蝥酸钠对体外培养的鼻咽癌CNE-2细胞株有增殖抑制作用,其作用与药物浓度、作用时间呈正相关性。 2、去甲斑蝥酸钠对体外培养的鼻咽癌CNE-2细胞株有放射增敏作用。 3、去甲斑蝥酸钠增加鼻咽癌CNE-2细胞放射敏感性的可能机制是阻滞细胞周期、诱导细胞凋亡。
[Abstract]:Objective to investigate the radiosensitizing effect of sodium norcantharidate (Disodium Cantharidinate) on nasopharyngeal carcinoma (NPC) CNE-2 cell line in vitro and its mechanism. Methods the inhibitory effects of norcantharidin sodium at different concentrations on the proliferation of nasopharyngeal carcinoma (NPC) CNE-2 cells were determined by MTS assay. The half inhibitory concentration (50%Inhibiting Concentration, IC50) was calculated. Nasopharyngeal carcinoma (NPC) CNE-2 cells were divided into three groups: blank control group, irradiation group, combined drug and irradiation group. The combined effect of sodium norcantharidate and radiation was studied by clone formation method. The curve was fitted by SF=1- (1-e-D/D0) 螖 N, a mathematical model of "multi-target click". Cell survival curve, average lethal dose, quasi-threshold dose and radiosensitization ratio (Sensitization enhancement ratio, SER) were obtained to evaluate the effects. Nasopharyngeal carcinoma (NPC) CNE-2 cells were divided into three groups: blank control group, irradiation group, drug and irradiation combined group. After 48 hours of treatment with sodium norcantharidate, apoptosis rate and cell cycle were analyzed by flow cytometry. Results the growth and proliferation of nasopharyngeal carcinoma CNE-2 cells were inhibited after treated with sodium norcantharidate. The radiosensitization ratio (SER) of 340mg/L.20mg/L norcantharidate sodium to 340mg/L.20mg/L norcantharidate sodium for 48 h was 1.43. After treated with sodium norcantharidate and radiotherapy in CNE-2 cell line, the cell cycle was detected by flow cytometry. The cell cycle of G 2 / M phase increased sharply from 8.26% to 48.3%. CNE-2 cells were irradiated by 4Gy and treated with 20mg/L sodium norcantharidate. The apoptotic rate was higher than that in the blank control group (12.68 and 5.37, respectively), while the apoptosis rate in the irradiation combined with sodium norcantharidate group was higher than that in the blank control group (18.84%). The difference was statistically significant (P0.05). Conclusion 1. Sodium norcantharidate can inhibit the proliferation of nasopharyngeal carcinoma (NPC) CNE-2 cell line in vitro. 2. Sodium norcantharidate has radiosensitizing effect on CNE-2 cell line of nasopharyngeal carcinoma in vitro. 3. The possible mechanism of sodium norcantharidate to increase radiosensitivity of CNE-2 cell line of nasopharyngeal carcinoma To block the cell cycle, Induce apoptosis.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63

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