正常兔眼脉络膜上腔注射酮咯酸氨丁三醇的视网膜毒性研究

发布时间：2018-10-11 18:33

【摘要】：目的探讨正常兔眼脉络膜上腔注射不同剂量酮咯酸氨丁三醇（ketorolac tromethamine，KT）的视网膜毒性。实验方法新西兰大白兔12只，随机分为A和B两组，每只兔子右眼脉络膜上腔注射不同剂量的KT（A组：3000ug/0.05ml，B组：6000ug/0.05ml），左眼全部注射0.9%生理盐水作为对照。注射前和注射后1、2、4周每只眼进行闪光全视网膜电图（ERG）检测，4周后所有动物处死摘除眼球做病理切片，在光学显微镜下分析视网膜组织结构改变。结果兔眼脉络膜上腔注射KT前及注射后1、2、4周，实验眼（右眼）和对照眼（左眼）ERG视杆细胞反应、视杆-视锥细胞最大混合反应、振荡电位、视锥细胞反应和30Hz闪烁反应的波形均正常，振幅和潜伏期均未出现异常改变，，经过统计学分析实验眼与对照眼没有差异（P0.05）。光学显微镜下观察视网膜组织结构，各组实验眼和对照眼视网膜各层组织形态学表现均正常，细胞排列紧密整齐，未见异形细胞、炎症反应以及视网膜出血等结构异常。结论兔眼脉络膜上腔注射KT3000ug和6000ug后，视网膜功能，组织形态未见异常。这个剂量在兔眼脉络膜上腔注射是安全的。另外可以证明，脉络膜上腔注射的给药方式也是安全的。
[Abstract]:Objective to investigate the retinal toxicity of different doses of ketoclalate aminobutanol (ketorolac tromethamine,KT) injected into the suprachoroidal cavity of normal rabbits. Methods Twelve New Zealand white rabbits were randomly divided into two groups: group A and group B. each rabbit was injected with different doses of KT (A in the suprachoroidal cavity of each rabbit's right eye: group 6000ug/0.05ml (3000ugr / 0.05ml), and 0.9% saline was injected into the left eye as control. Every eye was examined with flash electroretinogram (ERG) before injection and 4 weeks after injection. After 4 weeks, all animals were killed to remove their eyeballs to make pathological sections, and the changes of retinal tissue structure were analyzed under optical microscope. Results before and after KT injection, the ERG rod cells of experimental eyes (right eye) and control eyes (left eye), the maximum mixed reaction of rod and cone cells, and oscillatory potential were observed in experimental eyes (right eye) and control eyes (left eye) before and after injection of KT. The waveforms of cone cell reaction and 30Hz scintillation reaction were normal and the amplitude and latency were not abnormal. There was no difference between the experimental eye and the control eye after statistical analysis (P0.05). The structure of the retina was observed under optical microscope. The histomorphology of all layers of the retina of the experimental eyes and the control eyes were normal and the cells were arranged closely and neatly without abnormal structure such as abnormal cells inflammatory reaction and retinal haemorrhage. Conclusion there is no abnormal retinal function and histomorphology after injection of KT3000ug and 6000ug into the superior choroidal cavity of rabbit eyes. This dose is safe to inject into the superior choroidal cavity of the rabbit eye. It can also be proved that the administration of suprachoroidal injection is safe.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774

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