酶免疫组化和酶联免疫吸附试验在鼻咽癌普查中应用比较

发布时间：2018-10-14 16:13

【摘要】：目的为探讨酶免疫组化法(enzyme immunohistochemistry technique,IHCT)和酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)在鼻咽癌普查中的价值,并对两种检测方法的结果进行对比分析。方法 2013年8月分别应用酶免疫组化法和ELISA法同期检测3 843名健康人群的EB病毒抗原(酶免疫组化法)、EB病毒抗体(ELISA法:VCA-IgA和EBNA1-IgA);根据EB病毒抗体水平对该人群分层评估:阳性组和阴性组(酶免疫组化法);高危组、中危组和低危组(ELISA法)。结果酶免疫组化法阳性率为5.1%(196/3 843),ELISA法阳性率为9.7%(374/3843),两种方法均为阳性53例,阳性率差异有统计学意义(P0.01)。对酶免疫组化法阳性组和ELISA法高危组每6个月追踪随访至2015年7月31日,酶免疫组化法阳性组有7例确诊鼻咽癌,其中早期鼻咽癌(T1~2N0M0)6例,阴性组未确诊鼻咽癌;ELISA法高危组有1例确诊鼻咽癌,中危组(阳性组)299例患者有6例确诊鼻咽癌,低危组(阴性组)随访2年未发现鼻咽癌。结论 ELISA法联合酶免疫组化法有助于发现早期鼻咽癌,更适合鼻咽癌的普查。
[Abstract]:Objective to investigate the value of enzyme immunohistochemical method (enzyme immunohistochemistry technique,IHCT) and enzyme-linked immunosorbent assay (enzyme linked immunosorbent assay,ELISA) in the screening of nasopharyngeal carcinoma (NPC) and to compare the results of the two methods. Methods in August 2013, the EB virus antigens (ELISA: VCA-IgA and EBNA1-IgA) of 3 843 healthy subjects were detected by enzyme immunohistochemical method and ELISA method respectively, and the stratified evaluation was made according to the level of EB virus antibody. Assessment: positive and negative groups (enzyme immunohistochemical method); high-risk groups, Middle risk group and low risk group (ELISA method). Results the positive rate of enzyme immunohistochemical method was 5.1% (P < 0.01). The positive rate of enzyme immunohistochemical method was 9.7% (374 / 3843). The positive rate of the two methods was positive in 53 cases. The difference was statistically significant (P0.01). Seven cases of nasopharyngeal carcinoma (NPC) were diagnosed in the Elisa positive group and the ELISA high risk group every 6 months until July 31, 2015. Among them, 6 cases were early nasopharyngeal carcinoma (T1~2N0M0). Nasopharyngeal carcinoma was not diagnosed in negative group, nasopharyngeal carcinoma was diagnosed in high risk group by ELISA method, 6 cases were diagnosed in middle risk group (positive group), and no nasopharyngeal carcinoma was found in low risk group (negative group) for 2 years. Conclusion ELISA method combined with enzyme immunohistochemical method is helpful to find early nasopharyngeal carcinoma and is more suitable for general examination of nasopharyngeal carcinoma.
【作者单位】：广西壮族自治区人民医院耳鼻咽喉头颈肿瘤科鼻咽癌研究所;广西梧州市苍梧县鼻咽癌防治所;
【基金】：广西科学研究与技术开发计划项目(桂科攻14124003-3;桂科攻14279008)
【分类号】：R739.63

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