青蒿琥酯对人Tenon囊成纤维细胞增殖与凋亡的影响

发布时间：2018-10-15 19:46

【摘要】：目的观察青蒿琥酯(artesunate,Art)对人Tenon囊成纤维(human Tenon’s capsule fibroblasts,HTFs)细胞增殖与凋亡的影响,探讨青光眼术后滤过泡瘢痕化的治疗对策。方法体外培养HTFs细胞,应用不同浓度(50μg·mL~(-1)、100μg·mL~(-1)、150μg·mL~(-1)、200μg·mL~(-1))的Art处理细胞,采用MTT法检测Art对细胞增殖的影响,流式细胞仪检测Art对细胞凋亡的影响,Western blot检测Art对凋亡相关蛋白Bax、Bcl-2表达的影响。结果 Art作用细胞48 h后,MTT结果显示:与空白对照组比较,不同浓度的Art处理组对HTFs细胞的增殖均有抑制作用,且呈浓度依赖性抑制(均为P0.05)。流式细胞仪结果显示:与空白对照组比较,50μg·mL~(-1)、100μg·mL~(-1)、150μg·mL~(-1)、200μg·mL~(-1) Art作用后细胞凋亡率逐渐增加(均为P0.05),分别为8.80%±0.88%、11.60%±0.56%、16.30%±1.03%、23.40%±1.62%。Western blot检测结果显示:与空白对照组比较,不同浓度Art处理组Bax蛋白表达明显增加,Bcl-2蛋白表达明显降低,且均呈浓度依赖性(均为P0.05)。结论 Art可抑制HTFs细胞增殖并诱导其凋亡,其机制可能是通过调节凋亡相关蛋白Bax、Bcl-2的表达而实现的。Art可能成为一种潜在的抗青光眼术后滤过泡瘢痕化的药物。
[Abstract]:Objective to observe the effects of artesunate (artesunate,Art) on the proliferation and apoptosis of human Tenon (human Tenon's capsule fibroblasts,HTFs cells and to explore the therapeutic strategies of bleb scarring after glaucoma surgery. Methods HTFs cells were cultured in vitro and treated with Art of different concentrations (50 渭 g mL~ (-1), 100 渭 g mL~ (-1), 150 渭 g mL~ (-1), 200 渭 g mL~ (-1). MTT assay was used to detect the effect of Art on cell proliferation. Flow cytometry was used to detect the effect of Art on apoptosis. Results after 48 h of Art treatment, MTT results showed that the proliferation of HTFs cells was inhibited in a concentration-dependent manner in different concentrations of Art (P0.05) compared with the control group. The results of flow cytometry showed that the apoptotic rate of 50 渭 g mL~ (-1), 100 渭 g mL~ (-1), 150 渭 g mL~ (-1), 200 渭 g mL~ (-1) Art increased gradually (P0.05) after treatment with 50 渭 g mL~ (-1), 100 渭 g mL~ (-1), 200 渭 g mL~ (-1) Art, and the results were 8.80% 卤0.888.60% 卤0.5616.30% 卤1.030.40% 卤1.62%.Western blot, respectively. The expression of Bax protein was significantly increased and the expression of Bcl-2 protein was significantly decreased in different concentrations of Art in a dose-dependent manner (P0.05). Conclusion Art can inhibit the proliferation and induce apoptosis of HTFs cells by regulating the expression of apoptosis-related protein Bax,Bcl-2. Art may be a potential drug against bleb scarring after glaucoma surgery.
【作者单位】：广州军区广州总医院眼科;广州军区广州总医院培训中心;
【分类号】：R775

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