AhR和TGF-β1在伴或不伴过敏性鼻炎的鼻息肉中表达差异初步研究
发布时间：2018-10-20 12:23
【摘要】：研究背景:很多种因素参与慢性鼻-鼻窦炎(chronic rhino sinusitis,CRS)的组织重构过程,这其中就包括过敏因素、转化生长因子β1(Transforming Growth Factor-beta 1,TGF-β1)等。另外,芳香烃受体(aryl hydrocarbon receptor,AhR)也参与其中,但是目前其具体机制还尚不明确。目的:本研究的目的就是要通过检测AhR与TGF-β1在CRS中的表达来探究两者与过敏状态和组织重构的可能相关性。方法:收集四组病人共75例,包括CRS不伴鼻息肉(nasal polyps,NP)不伴过敏性鼻炎(allergic rhinitis,AR)组(CRSs NPs AR)20例,CRS伴鼻息肉伴AR组(CRSwNPw AR)20例,CRSwNP不伴AR组(CRSwNPs AR)20例和对照组15例。采用马松三色染色方法,来评价40例患者中胶原的表达情况;通过免疫组织化学染色(SP法)来观察AhR和TGF-β1在CRS中的分布与表达水平;及通过实时荧光定量检测方法(q RT-PCR)和蛋白质免疫印迹法(Western blotting)分别检测AhR和TGF-β1的核酸和蛋白质表达水平。结果:通过组织学染色,以及在核酸水平和蛋白质水平进行检测分析发现,在CRSs NPs AR组,胶原水平、AhR和TGF-β1的表达均高于CRSwNPs AR(p0.05)。但是,CRSwNPw AR与CRSwNPs AR相比,胶原水平和TGF-β1是增高的,而AhR表达水平是降低的。AhR和TGF-β1的表达与胶原表达水平呈现正相关性。结论:我们推测AhR也许与TGF-β1一样也参与到了CRSwNP的组织重构中。而在CRSwNPw AR中AhR的低表达可能与过敏性反应有关。AhR与TGF-β1在CRS组织重构中的相互作用还有待进一步研究探讨。
[Abstract]:Background: there are many factors involved in the process of tissue remodeling in chronic rhinosinusitis (chronic rhino sinusitis,CRS), including allergic factors, transforming growth factor 尾 1 (Transforming Growth Factor-beta 1 (TGF- 尾 1) and so on. In addition, the aromatic receptor (aryl hydrocarbon receptor,AhR is also involved, but its mechanism is still unclear. Aim: to explore the possible correlation between AhR and TGF- 尾 1 in CRS by detecting the expression of TGF- 尾 1 in CRS. Methods: 75 patients in four groups were collected, including 20 patients with CRS without nasal polyps (nasal polyps,NP) without allergic rhinitis (allergic rhinitis,AR), 20 patients with CRS with nasal polyps with AR (CRSwNPw AR), 20 patients with CRSwNP without AR (CRSwNPs AR) and 15 patients with control group (control group). The expression of collagen in 40 patients was evaluated by using Ma Song three-color staining method, and the distribution and expression of AhR and TGF- 尾 1 in CRS were observed by immunohistochemical staining (SP). The expression levels of nucleic acid and protein in AhR and TGF- 尾 1 were detected by real-time fluorescence quantitative detection (Q RT-PCR) and Western blot (Western blotting), respectively. Results: the expression of AhR and TGF- 尾 1 in CRSs NPs AR group was higher than that in CRSwNPs AR (group (p0.05). However, compared with CRSwNPs AR, the collagen level and TGF- 尾 1 in, CRSwNPw AR were increased, while the AhR expression was decreased. There was a positive correlation between the expression of AhR and TGF- 尾 1 and collagen expression. Conclusion: we speculate that AhR may be involved in the tissue remodeling of CRSwNP as well as TGF- 尾 1. However, the low expression of AhR in CRSwNPw AR may be related to allergic reaction. The interaction between AhR and TGF- 尾 1 in CRS tissue remodeling needs further study.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R765.41
本文编号：2283172
[Abstract]:Background: there are many factors involved in the process of tissue remodeling in chronic rhinosinusitis (chronic rhino sinusitis,CRS), including allergic factors, transforming growth factor 尾 1 (Transforming Growth Factor-beta 1 (TGF- 尾 1) and so on. In addition, the aromatic receptor (aryl hydrocarbon receptor,AhR is also involved, but its mechanism is still unclear. Aim: to explore the possible correlation between AhR and TGF- 尾 1 in CRS by detecting the expression of TGF- 尾 1 in CRS. Methods: 75 patients in four groups were collected, including 20 patients with CRS without nasal polyps (nasal polyps,NP) without allergic rhinitis (allergic rhinitis,AR), 20 patients with CRS with nasal polyps with AR (CRSwNPw AR), 20 patients with CRSwNP without AR (CRSwNPs AR) and 15 patients with control group (control group). The expression of collagen in 40 patients was evaluated by using Ma Song three-color staining method, and the distribution and expression of AhR and TGF- 尾 1 in CRS were observed by immunohistochemical staining (SP). The expression levels of nucleic acid and protein in AhR and TGF- 尾 1 were detected by real-time fluorescence quantitative detection (Q RT-PCR) and Western blot (Western blotting), respectively. Results: the expression of AhR and TGF- 尾 1 in CRSs NPs AR group was higher than that in CRSwNPs AR (group (p0.05). However, compared with CRSwNPs AR, the collagen level and TGF- 尾 1 in, CRSwNPw AR were increased, while the AhR expression was decreased. There was a positive correlation between the expression of AhR and TGF- 尾 1 and collagen expression. Conclusion: we speculate that AhR may be involved in the tissue remodeling of CRSwNP as well as TGF- 尾 1. However, the low expression of AhR in CRSwNPw AR may be related to allergic reaction. The interaction between AhR and TGF- 尾 1 in CRS tissue remodeling needs further study.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R765.41
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相关期刊论文 前2条
1 张田;陈剑秋;朱春生;李跟红;解翠丽;王英;贺祯;;变应性因素及变应性鼻炎与慢性鼻-鼻窦炎鼻息肉的相关性探讨[J];临床耳鼻咽喉头颈外科杂志;2014年17期
2 伯铭羽;王向东;王鸿;宋贝贝;李颖;范尔钟;张罗;;重塑相关因子在慢性鼻窦炎的表达[J];首都医科大学学报;2013年06期
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