槲皮素对翼状胬肉成纤维细胞增殖的影响

发布时间：2018-10-20 17:29

【摘要】：目的：探讨槲皮素对体外培养人翼状胬肉成纤维细胞(HPF)增殖作用的影响，寻找预防翼状胬肉的发生和减少翼状胬肉复发的新方法。方法：1、用0～160mg/L槲皮素作用体外培养的HPF，24、48、72小时后CCK-8法检测药物对HPF的影响。2、Hoechst33342染色法：正常细胞摄取Hoechst33342染料后呈现蓝色荧光，而凋亡细胞摄取Hoechst33342能力比正细胞强，呈强蓝色荧光。用0～160mg/L槲皮素作用体外培养的HPF，24小时后加入Hoechst33342染色，10分钟后在荧光显微镜下观察荧光强弱。结果：1、在20～80mg/L范围内槲皮素作用24h后能显著抑制HPF的增殖(P0.05)，，呈时间依赖性和浓度依赖性。在0～160mg/L范围内槲皮素作用48h、72h后能显著抑制HPF的增殖(P0.05)，呈时间依赖性和浓度依赖性。2、Hoechst33258染色法显示在10～160mg/L范围内槲皮素作用24小时后，随槲皮素剂量的增加细胞凋亡增加。结论：槲皮素可显著抑制HPF的增殖和促细胞凋亡。
[Abstract]:Objective: to investigate the effect of quercetin on the proliferation of human pterygium fibroblasts (HPF) in vitro and to find a new method to prevent the occurrence of pterygium and reduce the recurrence of pterygium. Methods: 1. CCK-8 method was used to detect the effect of 0~160mg/L quercetin on HPF after HPF,24,48,72 hours. 2Hoechst33342 staining method: normal cells showed blue fluorescence after uptake of Hoechst33342 dye, but apoptotic cells had stronger ability of Hoechst33342 uptake than positive cells. Strong blue fluorescence. The HPF,24 cultured in vitro was treated with 0~160mg/L quercetin for hours and then Hoechst33342 staining was added. The fluorescence intensity was observed under fluorescence microscope for 10 minutes. Results: 1. Quercetin could significantly inhibit the proliferation of HPF in the range of 20~80mg/L for 24 h (P0.05), in a time-and concentration-dependent manner. The proliferation of HPF was significantly inhibited by quercetin in the range of 0~160mg/L for 48 h or 72 h (P0.05) in a time-and concentration-dependent manner. (2) Hoechst33258 staining showed that the apoptosis of HPF increased with the increase of quercetin dose in the range of 10~160mg/L for 24 hours. Conclusion: quercetin can significantly inhibit the proliferation and promote apoptosis of HPF.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R777.33

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