海洛因对小鼠视网膜结构和抗氧化物酶的影响

发布时间：2018-10-22 11:00

【摘要】：目的探讨海洛因对小鼠视网膜结构的影响。方法 60只昆明小鼠随机分为对照组和海洛因组2组,海洛因组用递增剂量(2,3,4 g/L)连续(1~5、6~10和11~15 d)腹腔注射海洛因溶液15 d(每d 2次,每次0.2ml),对照组注射等量生理盐水。称量检测小鼠体重及眼球重的变化,用生物显微技术观察小鼠视网膜组织结构的变化,用比色法检测超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的变化,用免疫组织化学法检测Bax和肿瘤坏死因子α(TNF-α)蛋白的表达情况。结果海洛因组小鼠体重和眼球重量均低于对照组,小鼠视网膜组织均有不同程度的损伤,神经节细胞减少,出现空泡样变性,内核层和外核层结构紊乱,疏松成网状;视网膜组织SOD活性显著降低,MDA含量显著增加;Bax,TNF-α蛋白表达显著增加。结论海洛因会降低视网膜组织抗氧化能力,使组织脂质过氧化反应增加,诱导视网膜细胞凋亡和炎症的发生,对视网膜有一定的损伤效应。
[Abstract]:Objective to investigate the effect of heroin on the retinal structure of mice. Methods 60 Kunming mice were randomly divided into two groups: the control group and the heroin group. The heroin group was injected with the same amount of normal saline in the heroin group with an increasing dose (2 ~ 3g / L) of heroin solution for 15 days (2 times per day, 0.2ml per day) and continuous intraperitoneal injection of heroin solution for 15 days (1: 5, 6g / L, 1115 days), and the control group was given the same amount of normal saline. The weight and eyeball weight of mice were measured by weighing, the changes of retinal tissue structure of mice were observed by biological microscopy, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were detected by colorimetry. The expression of Bax and tumor necrosis factor 伪 (TNF- 伪) protein was detected by immunohistochemistry. Results the body weight and eyeball weight in heroin group were lower than those in control group. The retina of mice were damaged to some extent, ganglion cells decreased, vacuolar degeneration appeared, the structure of inner and outer nuclear layer was disordered and loosely reticulated. The activity of SOD and the expression of Bax,TNF- 伪 protein in retina were significantly decreased and the content of MDA was increased significantly. Conclusion heroin can decrease the antioxidant ability of retina, increase lipid peroxidation, induce apoptosis and inflammation of retinal cells, and have a certain damage effect on retina.
【作者单位】：西北师范大学生命科学学院;甘肃政法学院公安分院;西北师范大学学报编辑部;
【基金】：甘肃省自然科学基金(1107RJZA141) 兰州市社会发展项目(2013-3-72,2014-1-105)
【分类号】：R774.1

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