维生素D受体及其基因多态性与鼻咽癌的关联

发布时间：2018-10-25 11:01

【摘要】：目的:探讨维生素D受体(VDR)及其基因多态性与鼻咽癌的关联。方法:采用荧光定量PCR检测48例鼻咽癌患者(鼻咽癌组)和48例对照者(对照组)外周血单个核细胞中的VDR mRNA。运用TaqMan-MGB荧光探针PCR法检测所有研究对象的FokI和ApaI位点基因型。结果:鼻咽癌组VDR mRNA△Ct值为9.32±0.91,对照组为7.31±1.04,鼻咽癌组VDR mRNA表达量显著低于对照组(t=10.08,P0.01)。采用多因素Logistic回归分析,结果显示FokI点Ff基因型在鼻咽癌组中的分布显著高于对照组(调整OR=1.97,95%CI=1.33~2.91)。未发现ApaI位点各基因型在对照组和鼻咽癌组中分布存在差异。进一步采用单倍型分析,结果显示,与fa单倍型相比,Fa单倍型在对照组中的分布显著高于鼻咽癌组(调整OR=0.65,95%CI=0.48~0.87)。2组FokI位点不同基因型中VDR mRNA表达水平差异有统计学意义(F=194.5,P0.01)。结论:VDR基因遗传变异与鼻咽癌有密切关联。
[Abstract]:Objective: to investigate the association of vitamin D receptor (VDR) and its gene polymorphism with nasopharyngeal carcinoma. Methods: VDR mRNA. in peripheral blood mononuclear cells of 48 patients with nasopharyngeal carcinoma (NPC group) and 48 controls (control group) was detected by fluorescence quantitative PCR. The genotypes of FokI and ApaI loci in all subjects were detected by TaqMan-MGB fluorescence probe PCR method. Results: VDR mRNA Ct was 9.32 卤0.91in nasopharyngeal carcinoma group and 7.31 卤1.04in control group. The expression of VDR mRNA in nasopharyngeal carcinoma group was significantly lower than that in control group (t = 10.08, P0.01). Multivariate Logistic regression analysis showed that the distribution of FokI point Ff genotype in nasopharyngeal carcinoma was significantly higher than that in control group (adjusted OR=1.97,95%CI=1.33~2.91). There was no significant difference in the distribution of ApaI locus genotypes between control group and nasopharyngeal carcinoma group. The results of haplotype analysis showed that the distribution of Fa haplotype in control group was significantly higher than that in nasopharyngeal carcinoma group (adjusted OR=0.65,95%CI=0.48~0.87). There were significant differences in VDR mRNA expression in different genotypes of FokI locus between two groups (FF194.5, P0.01). Conclusion: the genetic variation of VDR gene is closely related to nasopharyngeal carcinoma.
【作者单位】：扬州大学附属医院肿瘤科;宁波市第一人民医院耳鼻咽喉科;扬州大学附属医院耳鼻咽喉科;
【分类号】：R739.63

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