TLR-2、TLR-4与大鼠角膜碱烧伤早期炎症反应相关性研究

发布时间：2018-10-29 10:41

【摘要】：目的动态观察大鼠角膜碱烧伤后Toll样受体-2(Toll like-receptor 2,TLR-2)、Toll样受体-4(Toll like-receptor 4,TLR-4)与炎性因子白细胞介素-1β(interleukin-1,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达情况及变化规律,分析Toll样受体家族(Toll like-receptor familys,TLRs)与炎性因子IL-1β、TNF-α的表达是否存在相关关系。方法选用健康SPF级SD大鼠40只,右眼为烧伤实验眼,左眼为正常对照眼,用浓度为1 mol·L-1Na OH建立Ⅲ级角膜碱烧伤模型(模型建立时及模型建立后出现大鼠角膜烧伤程度不是Ⅲ级、大鼠角膜穿孔或前房积血等情况,均予以剔除,最后随机选取32只符合条件的大鼠进行后续实验),随机分为碱烧伤后3d组、7d组、14d组、21d组,每组8眼。分别在模型制作后观察大鼠眼前节,评估其角膜炎症指数,然后摘取大鼠眼球,行HE染色,计算炎症细胞数,同时用Western blot蛋白检测法检测大鼠角膜中TLR-2、TLR-4、IL-1β、TNF-α表达情况,分析各组差异,评估TLRs与炎性因子的相关性。结果在正常大鼠角膜中可检测到TLR-2、TLR-4、IL-1β、TNF-α蛋白少量表达,碱烧伤后其表达量增加,并于碱烧伤后7d达到峰值,以后逐渐递减,各组间(3d组、7d组、14d组、21d组)比较差异均有统计学意义(均为P0.05)。相关性分析:TLR-2与IL-1β(r=0.986,P0.05)、TNF-α(r=0.986,P0.05)的表达量呈正相关,TLR-4与IL-1β(r=0.975,P0.05)、TNF-α(r=0.990,P0.05)的表达量亦呈正相关。结论TLRs参与并可能启动角膜碱烧伤的免疫炎症反应,介导炎性因子的产生。
[Abstract]:Objective to observe the relationship between Toll like receptor 4 (Toll like-receptor 4 TLR-2), Toll receptor 4) and inflammatory factor interleukin-1 尾 (interleukin-1,IL-1 尾) after alkali burn of cornea in rats. The expression of tumor necrosis factor- 伪 (TNF- 伪) and its changes were analyzed. The relationship between Toll like receptor family (Toll like-receptor familys,TLRs) and the expression of inflammatory cytokines IL-1 尾 and TNF- 伪 was analyzed. Methods Forty healthy SPF grade SD rats were selected. The right eye was burned experimental eye and the left eye was normal control eye. The corneal alkali burn model of grade 鈪，

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