玻璃体切除术中台盼蓝着染晶状体后囊膜的初步观察

发布时间：2018-11-15 13:57

【摘要】：目的: 观察台盼蓝(Trypan Blue)对晶状体后囊膜染色的影响，探索临床应用台盼蓝着染晶状体后囊膜的可行性。方法: 本实验采用对照实验的方法。选用健康成年新西兰白兔60只（120只眼），随机分为3组（实验1组，实验2组，对照组），每组20只，，在手术显微镜下，完整取出晶状体，撕下前囊膜和后囊膜，分别置入不同浓度的台盼蓝溶液中染色后行光镜和电镜检查，并将所获数据进行统计学处理。结果： 1.染色效果空白对照组晶体囊膜未染色保持透明；各实验小组前后囊膜均见着色，并随染色液浓度的增加，囊膜染色加深。 2.光镜观察光镜下，对照组晶状体前囊膜和后囊膜表面光滑，厚薄均匀，无细胞及其他覆盖物；台盼蓝染色后，前囊膜和后囊膜较染色前未见明显改变。 3.电镜观察透射电镜下，空白对照组中晶状体前囊膜和后囊膜由多层板层状结构组成，其中有颗粒状、纤维状物质和低电子密度背景，未经台盼蓝染色的前囊膜和后囊膜表面光滑，囊膜中见散在有大小不一的卵圆形透明区和较小的颗粒状物质。台盼蓝染色后晶状体前后囊膜的超微结构发生了改变，前囊膜和后囊膜内未见电子透明区域，囊膜表面出现凹凸不平的较大颗粒状物质，记为“台盼蓝染色阳性”。 4.统计学处理前囊膜中，台盼蓝染色阳性数实验1组，实验2组分别与对照组比较，Pa0.05，差异均有显著性；台盼蓝染色阳性数实验1组与实验2组比较，Pb0.05，两组差异无显著性。后囊膜中，台盼蓝染色阳性数实验1组，实验2组分别与对照组比较，Pc0.05，差异均有显著性；台盼蓝染色阳性数实验1组与实验2组比较，Pd0.05，两组差异无显著性。结论：低浓度台盼蓝能安全有效地将晶状体后囊膜染色，具有良好的临床应用前景。
[Abstract]:Objective: to observe the effect of Trypan blue (Trypan Blue) on posterior capsule staining and to explore the feasibility of Trypan blue staining on posterior capsule. Methods: the method of control experiment was used in this experiment. Sixty healthy adult New Zealand white rabbits (120 eyes) were randomly divided into 3 groups (experimental group 1, experimental group 2, control group), with 20 rabbits in each group. The samples were stained in different concentrations of Trypan blue solution and examined by light microscope and electron microscope, and the obtained data were statistically processed. Results: 1. In the blank control group, the capsular membrane was not stained transparently, and the anterior and posterior capsule of each experimental group were stained, and the staining deepened with the increase of the concentration of the dye solution. 2. Under light microscope, the surface of anterior capsule and posterior capsule of control group were smooth, thin and uniform, without cells and other coverings, but the anterior capsule and posterior capsule did not change obviously after trypan blue staining. 3. Under the transmission electron microscope, the anterior capsule and posterior capsule of the lens in the blank control group were composed of lamellar multilayer structure with granular, fibrous and low electron density background. The surface of the anterior capsule and posterior capsule without trypan blue staining was smooth. After Trypan blue staining, the ultrastructure of the posterior capsule of the lens was changed. There was no transparent area in the anterior capsule and posterior capsule, and a large granular substance was found on the surface of the capsule, which was recorded as "Trypan blue staining positive". 4. The number of Trypan blue positive staining in the anterior capsule of experimental group 1 and experimental group 2 were significantly different from those of control group. There was no significant difference in Pb0.05, between experimental group 1 and experimental group 2. In the posterior capsule, the number of Trypan blue positive number in experimental group 1 and experimental group 2 were significantly different from those in control group, but there was no significant difference in Pd0.05, between experimental group 1 and experimental group 2. Conclusion: low concentration Trypan blue can stain the posterior capsule of lens safely and effectively, and has a good clinical application prospect.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R779.6

【相似文献】