干扰素-γ、白介素-4及白介素-17在鼻息肉中的表达及其意义

发布时间：2018-11-24 18:08

【摘要】：目的：采用HE染色法观察人鼻息肉中嗜酸性粒细胞(EOS)及炎症细胞的浸润情况,采用免疫组化SABC染色法观察IFN-γ、IL-4及IL-17在鼻息肉中的表达情况。探讨Th1、Th2及Th17细胞在鼻息肉发生发展中的作用。研究对象及方法：对象：随机选取2009年12月-2011年12月在我院耳鼻咽喉科住院做鼻内镜手术的鼻息肉患者30例,作为实验组。其中男17例、女13例,年龄17-60岁,平均年龄41.7岁。鼻息肉切除术后所有鼻息肉标本均经2位以上病理科副主任医师核实,并且排除既往有鼻息肉病史、变应性鼻炎及哮喘病史,排除其他变态反应及全身病史；鼻中隔矫正术患者正常下鼻甲组织20例,作为正常对照组。其中男12例、女8例,年龄18-53岁,平均年龄31.6岁。并排除有变应性鼻炎史及其他全身病史。方法：全部标本经10%的福尔马林溶液固定,常规石蜡包埋,切片后做HE染色和免疫组化SABC染色。HE染色下观察嗜酸性粒细胞及炎症细胞浸润情况,两种组织间的数据均以均数±标准差(x±S)表示,行t检验。采用免疫组织化学法检测两种组织中IFN-γ、IL-4和IL-17在的表达情况。三种细胞因子在两种组织中的表达均以均数±标准差(x±S)表示,行t检验。用Pearson直线相分析EOS与三种细胞因子之间的相关性。结果： 1.人鼻息肉中嗜酸性粒细胞浸润数明显高于正常下鼻甲组(p0.01) 2.IFN-γ在鼻息肉中的表达与正常下鼻甲组之间无明显差异(p0.05)。 3.IL-4在鼻息肉中的表达明显高于正常下鼻甲组(p0.01)。 4.IL-17在鼻息肉中的表达明显高于正常下鼻甲组(p0.01)。 5.在鼻息肉组织中EOS与IFN-γ缺乏相关,相关系数r值=-0.206(p0.05)。 6.在鼻息肉组织中EOS与IL-4呈正相关,相关系数r值=0.392(p0.05)。 7.在鼻息肉组织中EOS与IL-17呈正相关,相关系数r值=0.421(p0.05)。结论：鼻息肉中IL-4和IL-17表达均明显增强,提示鼻息肉中Th2/Th17细胞占优势。鼻息肉中可见大量的EOS浸润,IL-4和IL-17可能参与了EOS的趋化和聚集,并在鼻息肉形成过程中发挥重要作用。
[Abstract]:Objective: to observe the infiltration of eosinophil (EOS) and inflammatory cells in human nasal polyps by HE staining, and to observe the expression of IFN- 纬, IL-4 and IL-17 in nasal polyps by immunohistochemical SABC staining. To investigate the role of Th1,Th2 and Th17 cells in the development of nasal polyps. Objects and methods: thirty patients with nasal polyps who were hospitalized in our department of otorhinolaryngology from December 2009 to December 2011 were selected as experimental group. There were 17 males and 13 females, aged 17-60 years, with an average age of 41.7 years. All nasal polyps were verified by more than 2 deputy chief pathologists after resection of nasal polyps, and the history of nasal polyps, allergic rhinitis and asthma, other allergic reactions and systemic history were excluded. 20 cases of normal inferior turbinate were used as normal control group. There were 12 males and 8 females, aged 18-53 years, with an average age of 31.6 years. A history of allergic rhinitis and other systemic diseases was excluded. Methods: all the specimens were fixed with 10% formalin solution and embedded in paraffin. The infiltration of eosinophils and inflammatory cells was observed by HE staining and immunohistochemical SABC staining. The data between the two tissues were expressed as mean 卤standard deviation (x 卤S), and t test was performed. Immunohistochemical method was used to detect the expression of IFN- 纬, IL-4 and IL-17 in the two tissues. The expression of three cytokines in both tissues was expressed as mean 卤standard deviation (x 卤S), and t-test was performed. The correlation between EOS and three cytokines was analyzed by Pearson linear phase. Results: 1. The number of eosinophil infiltration in human nasal polyps was significantly higher than that in normal inferior turbinate group (p0.01). 2. The expression of IFN- 纬 in nasal polyp was not significantly different from that in normal inferior turbinate group (p0.05). The expression of 3.IL-4 in nasal polyps was significantly higher than that in normal inferior turbinate group (p 0.01). The expression of 4.IL-17 in nasal polyps was significantly higher than that in normal inferior turbinate group (p 0.01). 5. There was no correlation between EOS and IFN- 纬 in nasal polyps (r =-0.206). 6. There was a positive correlation between EOS and IL-4 in nasal polyps (r = 0.392). 7. There was a positive correlation between EOS and IL-17 in nasal polyps (r = 0.421). Conclusion: the expression of IL-4 and IL-17 in nasal polyps was significantly increased, suggesting that Th2/Th17 cells were dominant in nasal polyps. A large number of EOS infiltrates can be seen in nasal polyps. IL-4 and IL-17 may be involved in the chemotaxis and aggregation of EOS and play an important role in the formation of nasal polyps.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R765.25

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