模拟高原缺氧环境对大鼠视网膜神经元的影响
发布时间：2018-11-28 09:27
【摘要】：目的：探讨利用低压舱模拟高原缺氧环境对视网膜神经元产生的影响，为了解高原环境下视网膜损伤提供理论依据。方法：将45只wistar实验大鼠，按随机数字表法分为：650m海拔高度组(乌鲁木齐所在海拔)，模拟3000m高度组，模拟5000m高度组，三组大鼠分别饲养7天(每天平均23.5小时)后测量视网膜电流图(electroretinogram, ERG)，，观察各组大鼠电镜、光镜下视网膜的形态学改变，并测定凋亡细胞数目。结果：1.F-ERG a，b，Ops各子波OP1，OP2，OP3波峰值潜伏时以及b波波幅、Ops总波幅，模拟5000m高度组与模拟3000m海拔高度组和650m海拔高度组比较都具有明显差异（P0.05）；而模拟3000m海拔高度组与650m海拔高度组相比较无明显差异（P0.05）；a波波幅三组间无差异（P0.05）。2.光镜、电镜观察：模拟5000m海拔高度组大鼠视网膜神经节细胞层、内颗粒层结构异常，模拟3000m海拔高度组大鼠视网膜结构接近正常3. TUNEL染色观察：模拟5000m海拔高度组大鼠视网膜凋亡细胞明显增多,神经节细胞层及内颗粒层均见大量凋亡细胞阳性表达,模拟3000m海拔高度组大鼠视网膜凋亡细胞较模拟5000m海拔高度组减少，两者差别具有统计学意义(P0.05)。结论：1、模拟高原缺氧环境下的大鼠视网膜神经元形态结构发生改变，主要集中在神经节细胞层、内核层及外核层。2、在利用低压舱模拟的高原缺氧环境下，大鼠视网膜电流图出现改变，随着海拔高度的增高，视网膜电流图的改变越明显。3、视网膜神经元发生凋亡，是模拟高原缺氧环境下大鼠视网膜神经节细胞及内、外核层神经元死亡的重要方式，且凋亡细胞数量亦随海拔高度的增高而增多。
[Abstract]:Aim: to explore the effect of hypobaric chamber on retinal neurons in high altitude anoxic environment, and to provide theoretical basis for understanding retinal injury in high altitude environment. Methods: 45 wistar experimental rats were randomly divided into three groups: 650m altitude group (Urumqi altitude), simulated 3000m altitude group and 5000m altitude group. After feeding for 7 days (mean 23.5 hours per day), the electroretinogram (electroretinogram, ERG),) of the three groups was used to observe the morphological changes of the retina and the number of apoptotic cells. Results: the peak latency of OP1,OP2,OP3 wave, the amplitude of b wave, the total amplitude of Ops, the simulated height of 5 000m and the group of simulated altitude of 3000 m and 650 m were significantly different from those of the group of simulated altitude of 3000m and 650m (P0.05). However, there was no significant difference between the simulated 3000m altitude group and 650m altitude group (P0.05); a wave amplitude between the three groups (P0.05). 2. The retinal ganglion cell layer and the inner granular layer were abnormal in the 5 000 m altitude group, and the retinal structure was close to normal 3 000 m altitude group. Observation of TUNEL staining: the number of apoptotic cells in retina of rats in the simulated altitude of 5000m was significantly increased, and a large number of apoptotic cells were expressed in the ganglion cell layer and the inner granular layer. The number of retinal apoptotic cells in the simulated 3000m altitude group was lower than that in the simulated 5000m altitude group, the difference was statistically significant (P0.05). Conclusion: 1. The morphologic changes of retinal neurons in rats under simulated hypoxia at high altitude were mainly concentrated in ganglion cell layer, inner core layer and outer nuclear layer. 2. The change of retinal current map was more obvious with the increase of altitude. 3. The retinal neurons were apoptotic, which was the retinal ganglion cells and the inner part of the retinal ganglion cells under the simulated high altitude hypoxia environment. The number of apoptotic cells increased with the increase of altitude.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1
本文编号：2362461
[Abstract]:Aim: to explore the effect of hypobaric chamber on retinal neurons in high altitude anoxic environment, and to provide theoretical basis for understanding retinal injury in high altitude environment. Methods: 45 wistar experimental rats were randomly divided into three groups: 650m altitude group (Urumqi altitude), simulated 3000m altitude group and 5000m altitude group. After feeding for 7 days (mean 23.5 hours per day), the electroretinogram (electroretinogram, ERG),) of the three groups was used to observe the morphological changes of the retina and the number of apoptotic cells. Results: the peak latency of OP1,OP2,OP3 wave, the amplitude of b wave, the total amplitude of Ops, the simulated height of 5 000m and the group of simulated altitude of 3000 m and 650 m were significantly different from those of the group of simulated altitude of 3000m and 650m (P0.05). However, there was no significant difference between the simulated 3000m altitude group and 650m altitude group (P0.05); a wave amplitude between the three groups (P0.05). 2. The retinal ganglion cell layer and the inner granular layer were abnormal in the 5 000 m altitude group, and the retinal structure was close to normal 3 000 m altitude group. Observation of TUNEL staining: the number of apoptotic cells in retina of rats in the simulated altitude of 5000m was significantly increased, and a large number of apoptotic cells were expressed in the ganglion cell layer and the inner granular layer. The number of retinal apoptotic cells in the simulated 3000m altitude group was lower than that in the simulated 5000m altitude group, the difference was statistically significant (P0.05). Conclusion: 1. The morphologic changes of retinal neurons in rats under simulated hypoxia at high altitude were mainly concentrated in ganglion cell layer, inner core layer and outer nuclear layer. 2. The change of retinal current map was more obvious with the increase of altitude. 3. The retinal neurons were apoptotic, which was the retinal ganglion cells and the inner part of the retinal ganglion cells under the simulated high altitude hypoxia environment. The number of apoptotic cells increased with the increase of altitude.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R774.1
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