鼻咽癌患者EBV抗体效价及EBV DNA载量的检测及意义
发布时间：2018-12-13 08:58
【摘要】：目的：动态检测鼻咽癌患者放疗前后血清EBV VCA-IgA和EA-IgA抗体效价及外周血单个核细胞EBV DNA载量的变化,探讨血清中EBV DNA载量及抗体效价变化在鼻咽癌疗效监测及预后评估中的意义。 方法：选择24例鼻咽癌患者作为研究对象,EBER1原位杂交筛选出EBV阳性患者,收集EBV阳性患者放疗前、放疗过程中及放疗结束后的血清及外周血单个核细胞标本；采用ELISA试剂盒和实时荧光定量PCR动态检测患者血清VCA-IgA和EA-IgA抗体效价,以及外周血单个核细胞EBV DNA载量,分析随放疗的进行EBV特异性抗体及EBV DNA载量的变化。 结果：①22例鼻咽癌病人血清VCA-IgA、 EA-IgA抗体和EBV DNA在3个不同放疗阶段之间的差异均有统计学意义(F=3.305,8.914,9.461,P0.05)。放疗结束后VCA-IgA抗体水平显著低于放疗前水平(t=2.081,P0.05),而放疗中与放疗前及放疗结束后比较差异均无显著性(t=0.33,0.47,P0.05)。②EA-IgA抗体水平在放疗结束后显著低于放疗前,而放疗前与放疗中及放疗后比较差异均无统计学意义(t=0.606,0.50,P0.05)。③7例病人从治疗开始至治疗结束均未检测到EBV DNA,其余15例病人EBV DNA载量放疗前与放中及放疗结束后比较差异均有显著性(t=2.47、4.34,P0.05),而放疗中和放疗结束后比较差异无显著性(t=-1.86,P0.05)。 结论：①动态观察鼻咽癌患者EBV VCA-IgA和EA-IgA抗体效价以及外周血EBV DNA载量变化对评估鼻咽癌疗效均具有实用价值,其中EBV DNA载量变化较VCA-IgA和EA-IgA抗体效价变化更为敏感。②动态观察EBV DNA载量变化在鼻咽癌疗效监测方面的价值明显优于VCA-IgA及EA-IgA抗体检测,EBV DNA载量和特异性抗体的联合检测、综合分析有助于客观评价鼻咽癌患者放疗效果和预测患者预后。
[Abstract]:Objective: to investigate the changes of serum EBV VCA-IgA and EA-IgA antibody titers and the EBV DNA load of peripheral blood mononuclear cells (PBMC) in patients with nasopharyngeal carcinoma before and after radiotherapy. To investigate the significance of serum EBV DNA load and antibody titer in monitoring the curative effect and evaluating prognosis of nasopharyngeal carcinoma (NPC). Methods: 24 patients with nasopharyngeal carcinoma were selected and EBV positive patients were screened by EBER1 in situ hybridization. The samples of serum and peripheral blood mononuclear cells were collected from EBV positive patients before, during and after radiotherapy. The serum VCA-IgA and EA-IgA antibody titers and the EBV DNA load of peripheral blood mononuclear cells (PBMC) were detected by ELISA kit and real-time fluorescence quantitative PCR. The changes of EBV specific antibody and EBV DNA load with radiotherapy were analyzed. Results: there were significant differences in serum VCA-IgA, EA-IgA antibody and EBV DNA between patients with nasopharyngeal carcinoma in three different radiotherapy stages (FF3. 305, 8. 914, 9. 461 P 0.05). The level of VCA-IgA antibody after radiotherapy was significantly lower than that before radiotherapy (t = 2.081p0.05), but there was no significant difference between radiotherapy and after radiotherapy (t = 0.33 卤0.47, P < 0.05). The level of 2EA-IgA antibody after radiotherapy was significantly lower than that before radiotherapy, but there was no significant difference between before and after radiotherapy (t = 0.606 卤0.50, P < 0.05). From the beginning of treatment to the end of treatment, no EBV DNA, was detected in the other 15 patients. There was significant difference in EBV DNA load before radiotherapy and after radiotherapy in 37 patients (t = 2.47 or 4.34 P 0.05). However, there was no significant difference after radiotherapy and radiotherapy (tr-1.86 P 0.05). Conclusion: 1 dynamic observation of the antibody titers of EBV VCA-IgA and EA-IgA and the changes of EBV DNA load in peripheral blood of patients with nasopharyngeal carcinoma have practical value in evaluating the curative effect of nasopharyngeal carcinoma. The change of EBV DNA load was more sensitive than that of VCA-IgA and EA-IgA antibody titers. 2 the value of dynamic observation of EBV DNA load in monitoring the curative effect of nasopharyngeal carcinoma was significantly better than that of VCA-IgA and EA-IgA antibody detection. The combined detection of EBV DNA load and specific antibody is helpful to evaluate the radiotherapy effect and predict the prognosis of nasopharyngeal carcinoma patients.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63
本文编号：2376279
[Abstract]:Objective: to investigate the changes of serum EBV VCA-IgA and EA-IgA antibody titers and the EBV DNA load of peripheral blood mononuclear cells (PBMC) in patients with nasopharyngeal carcinoma before and after radiotherapy. To investigate the significance of serum EBV DNA load and antibody titer in monitoring the curative effect and evaluating prognosis of nasopharyngeal carcinoma (NPC). Methods: 24 patients with nasopharyngeal carcinoma were selected and EBV positive patients were screened by EBER1 in situ hybridization. The samples of serum and peripheral blood mononuclear cells were collected from EBV positive patients before, during and after radiotherapy. The serum VCA-IgA and EA-IgA antibody titers and the EBV DNA load of peripheral blood mononuclear cells (PBMC) were detected by ELISA kit and real-time fluorescence quantitative PCR. The changes of EBV specific antibody and EBV DNA load with radiotherapy were analyzed. Results: there were significant differences in serum VCA-IgA, EA-IgA antibody and EBV DNA between patients with nasopharyngeal carcinoma in three different radiotherapy stages (FF3. 305, 8. 914, 9. 461 P 0.05). The level of VCA-IgA antibody after radiotherapy was significantly lower than that before radiotherapy (t = 2.081p0.05), but there was no significant difference between radiotherapy and after radiotherapy (t = 0.33 卤0.47, P < 0.05). The level of 2EA-IgA antibody after radiotherapy was significantly lower than that before radiotherapy, but there was no significant difference between before and after radiotherapy (t = 0.606 卤0.50, P < 0.05). From the beginning of treatment to the end of treatment, no EBV DNA, was detected in the other 15 patients. There was significant difference in EBV DNA load before radiotherapy and after radiotherapy in 37 patients (t = 2.47 or 4.34 P 0.05). However, there was no significant difference after radiotherapy and radiotherapy (tr-1.86 P 0.05). Conclusion: 1 dynamic observation of the antibody titers of EBV VCA-IgA and EA-IgA and the changes of EBV DNA load in peripheral blood of patients with nasopharyngeal carcinoma have practical value in evaluating the curative effect of nasopharyngeal carcinoma. The change of EBV DNA load was more sensitive than that of VCA-IgA and EA-IgA antibody titers. 2 the value of dynamic observation of EBV DNA load in monitoring the curative effect of nasopharyngeal carcinoma was significantly better than that of VCA-IgA and EA-IgA antibody detection. The combined detection of EBV DNA load and specific antibody is helpful to evaluate the radiotherapy effect and predict the prognosis of nasopharyngeal carcinoma patients.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63
【引证文献】
相关硕士学位论文 前1条
1 阮开安;鼻咽癌放疗后局部复发的影像学分析[D];广西医科大学;2013年
，本文编号：2376279
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