叶黄素对人眼球筋膜囊成纤维细胞增殖及凋亡的影响
发布时间：2018-12-17 21:08
【摘要】：目的探讨叶黄素（Lutein）对体外培养的人眼球筋膜囊成纤维细胞增殖及凋亡的影响。 方法对体外培养的人Tenon氏囊成纤维细胞，，用5～40ug/mlLutein作用12、24、48h后采用CCK-8法检测Lutein对成纤维细胞增殖的影响；5～40ug/mlLutein对人Tenon氏囊成纤维细胞作用24h后采用FCM检测Lutein对成纤维细胞细胞周期的影响，用Hoechst33258染色法检测Lutein对成纤维细胞凋亡的影响。 结果 1.CCK-8法显示不同浓度的Lutein（5、10、20、40ug/ml）对体外培养的人Tenon囊成纤维细胞作用12、24、48h后，较对照组可以明显抑制细胞增殖（P㩳0.05），且随着时间和浓度的增加，Lutein对细胞的抑制作用增强； 2.流式细胞仪检测显示5～40ug/mlLutein对成纤维细胞作用24h后能够将成纤维细胞阻滞在G0/G1期，进入S期的细胞减少，且随着Lutein浓度的增加，滞留在G0/G1期的细胞也明显增加（P㩳0.05）； 3.Hoechst33258染色法显示5～40ug/mlLutein对成纤维细胞作用24h后有促进细胞凋亡的作用，且随着Lutein浓度的增加，凋亡率逐渐增加（P㩳0.05）。 结论 1.Lutein能够抑制体外培养的人Tenon氏囊成纤维细胞的增殖以及促进其凋亡。 2.关于Lutein在青光眼滤过术后抗滤过泡瘢痕化的作用及其机制值得进一步研究。
[Abstract]:Objective to investigate the effect of lutein (Lutein) on proliferation and apoptosis of cultured human ocular fascia fibroblasts. Methods Human Tenon's capsule fibroblasts cultured in vitro were treated with 5~40ug/mlLutein for 48 h. The effect of Lutein on proliferation of fibroblasts was detected by CCK-8 assay. The effect of 5~40ug/mlLutein on the fibroblast of Tenon's capsule was detected by FCM after 24 h, and the effect of Lutein on the apoptosis of fibroblast was detected by Hoechst33258 staining. Results 1.CCK-8 assay showed that different concentrations of Lutein (510 ~ 20g / ml) could significantly inhibit the proliferation of human Tenon capsule fibroblasts cultured in vitro for 48 h (P0. 05), and with the increase of time and concentration, the proliferation of the cells was inhibited significantly compared with the control group (P0. 05). The inhibitory effect of Lutein on cells was enhanced. 2. Flow cytometry showed that 5~40ug/mlLutein could block fibroblasts in G0/G1 phase after 24 hours of treatment, and the number of cells entering S phase decreased with the increase of Lutein concentration. The number of cells stranded in G0/G1 phase was also significantly increased (P0. 05). 3.Hoechst33258 staining showed that 5~40ug/mlLutein could promote the apoptosis of fibroblasts after 24 hours of treatment, and the apoptosis rate increased with the increase of Lutein concentration (P0. 05). Conclusion 1.Lutein can inhibit the proliferation and promote the apoptosis of human Tenon's capsule fibroblasts in vitro. 2. The role of Lutein in anti-scarring of filtering bleb after glaucoma filtration and its mechanism are worthy of further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R775
本文编号：2384799
[Abstract]:Objective to investigate the effect of lutein (Lutein) on proliferation and apoptosis of cultured human ocular fascia fibroblasts. Methods Human Tenon's capsule fibroblasts cultured in vitro were treated with 5~40ug/mlLutein for 48 h. The effect of Lutein on proliferation of fibroblasts was detected by CCK-8 assay. The effect of 5~40ug/mlLutein on the fibroblast of Tenon's capsule was detected by FCM after 24 h, and the effect of Lutein on the apoptosis of fibroblast was detected by Hoechst33258 staining. Results 1.CCK-8 assay showed that different concentrations of Lutein (510 ~ 20g / ml) could significantly inhibit the proliferation of human Tenon capsule fibroblasts cultured in vitro for 48 h (P0. 05), and with the increase of time and concentration, the proliferation of the cells was inhibited significantly compared with the control group (P0. 05). The inhibitory effect of Lutein on cells was enhanced. 2. Flow cytometry showed that 5~40ug/mlLutein could block fibroblasts in G0/G1 phase after 24 hours of treatment, and the number of cells entering S phase decreased with the increase of Lutein concentration. The number of cells stranded in G0/G1 phase was also significantly increased (P0. 05). 3.Hoechst33258 staining showed that 5~40ug/mlLutein could promote the apoptosis of fibroblasts after 24 hours of treatment, and the apoptosis rate increased with the increase of Lutein concentration (P0. 05). Conclusion 1.Lutein can inhibit the proliferation and promote the apoptosis of human Tenon's capsule fibroblasts in vitro. 2. The role of Lutein in anti-scarring of filtering bleb after glaucoma filtration and its mechanism are worthy of further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R775
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